Pharmacological investigation of hydrogen sulfide (H2S) contractile activity in rat detrusor muscle

Patacchini, Riccardo; Santicioli, Paolo; Giuliani, Sandro; Maggi, Carlo Alberto

doi:10.1016/j.ejphar.2005.01.005

Cited by 78 publications

(70 citation statements)

References 38 publications

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“…[20][21][22] We observed in the midst of H 2 S, the relaxation of smooth muscle precontracted with acetylcholine chloride. Streng et al 20 investigated the transient receptor potential vanilloid receptor 1 and showed that it might be involved in the contraction induced by NaHS when rat bladder strips were electrically stimulated.…”

Section: Discussionmentioning

confidence: 93%

“…Streng et al 20 investigated the transient receptor potential vanilloid receptor 1 and showed that it might be involved in the contraction induced by NaHS when rat bladder strips were electrically stimulated. Patacchini et al 21 reported Expression and function of hydrogen sulphide in human and rat bladder JW Gai et al 694 that intravesical NaHS instillation increased protamine sulphate pretreated rat bladder pressure. Dombkowski et al 22 explored the H 2 S effects on trout bladder smooth muscle and suggested that H 2 S could produce a dose-dependent relaxation in unstimulated and carbachol pre-contracted bladders and inhibit spontaneous contractions.…”

Section: Discussionmentioning

confidence: 99%

“…A preparation of 500 ml of tissue homogenate mixed with 500 ml of pre-cooled 50 mmol l 21 pH 6.8 phosphate-buffered saline and 1 ml of reacting system solution (100 mmol l 21 pH 7.4 phosphate-buffered saline, 10 mmol l 21 L-Cys (Sigma-Aldrich), 2 mmol l 21 phosphate pyridoxine aldehyde) was made. Then, 2 ml of the mixed solution was moved to the flask outer room after the sample protein concentration was determined, and 1 ml of 1 mol l 21 NaOH was added to the central room of the bottle. The sealed flask was incubated at 37uC water bath for 90 min, and then 1 ml of 50% trichloroacetic acid was added to end the reaction.…”

Section: Endogenous H 2 S Productivity Measurementsmentioning

confidence: 99%

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Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Gai

Wahafu²,

Guo³

et al. 2013

Asian J Androl

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We investigated the expression of hydrogen sulphide (H 2 S) in human and rat lower urinary tract (including bladder, prostate and urethra) tissues, and we sought to determine whether H 2 S induces relaxation of human and Sprague-Dawley (SD) rat bladder strips. Human normal lower urinary tract tissue was obtained for the evaluation of endogenous H 2 S productivity using a sulphide-sensitive electrode and for the analysis of the expression levels of all three synthases of endogenous H 2 S, cystathionine b-synthase (CBS), cystathionine c lyase (CSE) and 3-mercaptopyruvate sulphur transferase (MPST, as known as 3-MST) by Western blot assay. CBS, CSE and MPST were located in human sample slides by immunohistochemistry. Human and male adult SD rat bladder strips were tested for H 2 S function with a transducer and recorded. All experiments were repeated six times. The endogenous H 2 S productivity and the H 2 S synthases had various distributions in the human and rat lower urinary tract tissues and were located in both epithelial and stromal sections. L-cysteine (L-Cys, a substrate of CBS, CSE and MPST) elicited relaxation in a dose-dependent manner on human bladder strips pre-contracted by acetylcholine chloride. This effect could be diminished by the ATP-sensitive potassium ion (K ATP ) channel blocker glibenclamide (GLB), the CSE inhibitor DL-propargylglycine (PPG) and the CBS inhibitor hydroxylamine (HA). H 2 S and its three synthases were present in the human and rat lower urinary tract tissues and relaxed human and rat bladder strips, which implied that endogenous H 2 S might play a role in physiological function and pathological disorders of the lower urinary tract symptoms (LUTS) or overactive bladder (OAB).

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Endogenous H 2 S Productivity Measurementsmentioning

confidence: 99%

See 1 more Smart Citation

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Gai

Wahafu²,

Guo³

et al. 2013

Asian J Androl

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“…Furthermore, in the isolated guinea pig airway, NaSH induced the release of SP and CGRP from sensory nerve terminals, and led to airway constriction through capsaicin and NKR1/NKR2-transient receptor potential vanilloid 1 (TRPV1)-dependent pathways [73,74]. Similarly, in isolated rat bladder [75] and rat detrusor muscle [76], NaSH induced tachykin release from caspaicin-sensitive primary afferent neurons, leading to smooth muscle contraction. Collectively, these studies suggest that induction of neurogenic inflammation could mediate the reported proinflammatory role of H 2 S in inflammatory pathologies.…”

Section: Role For H 2 S In Neurogenic Inflammation?mentioning

confidence: 99%

Hydrogen sulfide and inflammation: the good, the bad, the ugly and the promising

Whiteman

Winyard

2011

Expert Review of Clinical Pharmacology

277

232

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“…So we tested the protein and mRNA expression of SUR2B and Kir6.1 in aortic and pulmonary arteries to investigate the possible mechanisms responsible for the regulation of vasorelaxation by H 2 S. H 2 S deficiency has been observed in animal models of systemic and pulmonary hypertension [16][17][18] . It also plays important roles in the pathogenesis of cardiovascular diseases [16][17][18][19][20][21][22][23][24] . The main mechanism for the cardiovascular actions of H 2 S was considered to be the activation of K ATP channels, because H 2 S increased whole-cell K ATP channel currents in rat aortic vascular smooth muscle cells [15] .…”

Section: Wwwchinapharcom Sun Y Et Almentioning

confidence: 99%

The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings

et al. 2011

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Aim:To compare the vasorelaxing effects of hydrogen sulfide (H 2 S) on isolated aortic and pulmonary artery rings and to determine their action mechanisms. Methods: H 2 S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95% O 2 and 5% CO 2 was analyzed. The expression of cystathinonine gamma-lyase (CSE), cystathionine beta synthase (CBS), 3-mercaptopyruvate sulfurtransferase (3MST), SUR2B and Kir6.1 was examined. Results: NaHS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NaHS dilated aortic rings to a greater extent (16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 μmol/L, respectively) than pulmonary artery rings (10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 μmol/L, respec tively). The EC 50 of the vasorelaxant effect for aortic rings was 152.17 μmol/L, whereas the EC 50 for pulmonary artery rings was 233.65 μmol/L. The vasorelaxing effect of H 2 S was markedly blocked b y cellular and mitochondrial membrane K ATP channel blockers in aortic rings (P<0.01). In contrast, only the cellular membrane K ATP channel blocker inhibited H 2 S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase (CSE) but not cystathionine beta synthase (CBS) expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase (3MST) mRNA was lower in aortic rings than in pulmonary artery rings. Conclusion: The vasorelaxing effect of H 2 S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the K ATP channel subunit SUR2B.

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Pharmacological investigation of hydrogen sulfide (H2S) contractile activity in rat detrusor muscle

Cited by 78 publications

References 38 publications

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Hydrogen sulfide and inflammation: the good, the bad, the ugly and the promising

The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings

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