Pharmacological influence of antirheumatic drugs on proteoglycanases from interleukin-1 treated articular cartilage

Steinmeyer, Jürgen; Daufeldt, Sabine

doi:10.1016/s0006-2952(97)00066-x

Cited by 14 publications

(7 citation statements)

References 42 publications

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“…These positive influences help protect against cartilage degradation in inflamed arthritic joints and osteoarthritis 46, 47. Chondrocytes maintain cartilage tissue homeostasis, sustaining the crucial balance between the rate of biosynthesis and incorporation of matrix components, and the rate of their degradation and subsequent loss from the cartilage into the synovial fluid 48. The present results show that hydrogel composites improved mRNA expression of CB1 compared with control.…”

Section: Discussionmentioning

confidence: 51%

Platelet‐rich plasma loaded in situ‐formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation

Lee

Park

Joung

et al. 2012

J Biomedical Materials Res

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The efficacy of three‐dimensional (3D) culture on the proliferation and maturation of chondrocytes seeded into a hydrogel scaffold was assessed. Three types of hydrogel were prepared for the 3D culture of primary isolated chondrocytes. Chondrocyte proliferation was assessed using a live/dead viability/cytotoxicity assay and semiquantitative RT‐PCR after 3D culture in hydrogel. Cylindrical defects in the center of rat xyphoids were used for the implantation of platelet‐rich plasma (PRP)/hydrogel composites. Rats were killed at day 7 postoperatively and evaluated histochemically and immunohistologically. Xyphoid chondrocytes proliferated well with time in hydrogels. In the PRP‐containing hydrogels, xyphoid defects displayed early formation of chondroid matrix with massive peripheral infiltration of spindle cells. These results were consistent with Safranin‐O staining for proteoglycans and immunohistochemistry for type II collagen. Gene expression analyses in vitro revealed aggrecan, type II collagen, and ChM‐1 and CB1 upregulation by PRP/hydrogel. PRP/hydrogel provided a suitable environment for hyaline cartilaginous regeneration, leading to anti‐inflammation by significant increase of CB1 and inhibiting vascular ingrowth via considerable upregulation of ChM‐1. The results provide a valuable reference for the clinical application of hydrogel scaffolds for hyaline cartilage regeneration, as well as the use of autologous PRP to improve cellular proliferation and maturation of xyphoid repair. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3099–3107, 2012.

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Section: Discussionmentioning

confidence: 51%

Platelet‐rich plasma loaded in situ‐formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation

Lee

Park

Joung

et al. 2012

J Biomedical Materials Res

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“…Recently, Pelletier et al reported that the NSAID, carprofen, reduced the structural changes and abnormal bone metabolism in an experimental dog OA model (51). In addition, some reports have shown positive effects of NSAIDs on cartilage integrity through suppression of proteoglycan degradation in vitro (52, 53) and in vivo (54, 55). In contrast, several reports indicate that these compounds do not have any positive effect on IL‐1β‐stimulated proteoglycan degradation (38, 53).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, some reports have shown positive effects of NSAIDs on cartilage integrity through suppression of proteoglycan degradation in vitro (52, 53) and in vivo (54, 55). In contrast, several reports indicate that these compounds do not have any positive effect on IL‐1β‐stimulated proteoglycan degradation (38, 53). NSAIDs have also been reported to exert negative effects on proteoglycan synthesis, leading some to caution that long‐term treatment of OA with NSAIDs may have adverse effects on cartilage integrity.…”

Section: Discussionmentioning

confidence: 99%

Cyclooxygenase 2‐dependent prostaglandin E₂ modulates cartilage proteoglycan degradation in human osteoarthritis explants

Hardy¹,

Seibert²,

Manning³

et al. 2002

Arthritis & Rheumatism

287

210

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Objective To examine cyclooxygenase‐2 (COX‐2) enzyme expression, its regulation by interleukin‐1β (IL‐1β), and the role of prostaglandin E2 (PGE2) in proteoglycan degradation in human osteoarthritic (OA) cartilage. Methods Samples of human OA articular cartilage, meniscus, synovial membrane, and osteophytic fibrocartilage were obtained at knee arthroplasty and cultured ex vivo with or without IL‐1β and COX inhibitors. COX expression was evaluated by immunohistochemistry and Western blot analysis. The enzymatic activity of COX was measured by conversion of arachidonic acid to PGE2. Cartilage degradation was evaluated by measuring the accumulation of sulfated glycosaminoglycans in the medium. Results IL‐1β induced robust expression of COX‐2 and PGE2 in OA meniscus, synovial membrane, and osteophytic fibrocartilage explants, whereas low levels were produced in OA articular cartilage. IL‐1β also induced cartilage proteoglycan degradation in OA synovial membrane‐cartilage cocultures. Increased proteoglycan degradation corresponded to the induction of COX‐2 protein expression in, and PGE2 production from, the synovial membrane. Dexamethasone, neutralizing IL‐1β antibody, or the selective COX‐2 inhibitor, SC‐236, attenuated both the IL‐1β‐induced PGE2 production and cartilage proteoglycan degradation in these cocultures. The addition of PGE2 reversed the inhibition of proteoglycan degradation caused by SC‐236. Conclusion IL‐1β‐induced production of COX‐2 protein and PGE2 was low in OA articular cartilage compared with that in the other OA tissues examined. IL‐1β‐mediated degradation of cartilage proteoglycans in OA synovial membrane‐cartilage cocultures was blocked by the selective COX‐2 inhibitor, SC‐236, and the effect of SC‐236 was reversed by the addition of exogenous PGE2. Our data suggest that induction of synovial COX‐2‐produced PGE2 is one mechanism by which IL‐1β modulates cartilage proteoglycan degradation in OA.

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“…Among the many tissues in the body, bone has some potential for repair, however cartilage lacks effective endogenous repair mechanisms, thus making it vulnerable to damage due to ‘wear and tear’ or an underlying pathology ‘failure to repair’. Chondrocytes serve to maintain cartilage tissue homeostasis, maintaining the crucial balance between the rate of biosynthesis and incorporation of matrix components, and the rate of their degradation and subsequent loss from the cartilage into the synovial fluid [41]. Active proteinases such as aggrecans, collageneses and matrix metalloproteinases (MMPs) become involved during cartilage resorption, degrading major components of the cartilage extracellular matrix, such as collagen type II and proteoglycans (mainly aggrecan) [42].…”

Section: Tissue Engineering Strategies To Solve Cartilage Diseasementioning

confidence: 99%

Tissue Engineering of Cartilage; Can Cannabinoids Help?

et al. 2010

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This review discusses the role of the cannabinoid system in cartilage tissue and endeavors to establish if targeting the cannabinoid system has potential in mesenchymal stem cell based tissue-engineered cartilage repair strategies. The review discusses the potential of cannabinoids to protect against the degradation of cartilage in inflamed arthritic joints and the influence of cannabinoids on the chondrocyte precursors, mesenchymal stem cells (MSCs). We provide experimental evidence to show that activation of the cannabinoid system enhances the survival, migration and chondrogenic differentiation of MSCs, which are three major tenets behind the success of a cell-based tissue-engineered cartilage repair strategy. These findings highlight the potential for cannabinoids to provide a dual function by acting as anti-inflammatory agents as well as regulators of MSC biology in order to enhance tissue engineering strategies aimed at cartilage repair.

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Pharmacological influence of antirheumatic drugs on proteoglycanases from interleukin-1 treated articular cartilage

Cited by 14 publications

References 42 publications

Platelet‐rich plasma loaded in situ‐formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation

Platelet‐rich plasma loaded in situ‐formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation

Cyclooxygenase 2‐dependent prostaglandin E₂ modulates cartilage proteoglycan degradation in human osteoarthritis explants

Tissue Engineering of Cartilage; Can Cannabinoids Help?

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Pharmacological influence of antirheumatic drugs on proteoglycanases from interleukin-1 treated articular cartilage

Cited by 14 publications

References 42 publications

Platelet‐rich plasma loaded in situ‐formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation

Platelet‐rich plasma loaded in situ‐formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation

Cyclooxygenase 2‐dependent prostaglandin E2 modulates cartilage proteoglycan degradation in human osteoarthritis explants

Tissue Engineering of Cartilage; Can Cannabinoids Help?

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Cyclooxygenase 2‐dependent prostaglandin E₂ modulates cartilage proteoglycan degradation in human osteoarthritis explants