Pharmacological evidence for the participation of NO–cyclic GMP–PKG–K+ channel pathway in the antiallodynic action of resveratrol

Bermúdez-Ocaña, Deysi Y.; Ambriz-Tututi, Mónica; Pérez-Severiano, Francisca; Granados‐Soto, Vinicio

doi:10.1016/j.pbb.2006.06.019

Cited by 37 publications

(23 citation statements)

References 68 publications

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“…The IT administration of resveratrol, a Sirt1 agonist, delayed and attenuated CCI-induced pain hypersensitivity, and resveratrol also decreased acetyl-histone H3 and increased Sirt1 expression in the spinal cord. Thus, we have extended previous observations 13Y17, 19 by showing that resveratrol significantly reduced spinal nerve ligationYinduced tactile allodynia via the activation of spinal Sirt1. To our knowledge, this is the first report on the spinal III histone/protein deacetylases involved in the antiallodynic effect of resveratrol in a neuropathic pain model.…”

Section: Discussionsupporting

confidence: 87%

“…The location of the distal end of the IT catheter was verified at the end of each experiment by visual inspection after the animals were killed. The doses of IT resveratrol were selected according to a previous study 19 and our preliminary experiments.…”

Section: Lumbosacral Intrathecal Surgery and Drug Injectionmentioning

confidence: 99%

“…14Y17 Resveratrol also stimulates the activity of large-conductance Ca 2+ -activated K + channels in cultured endothelial cells, 18 and this effect has been associated with its antinociceptive effects in the formalin model 16 and neuropathic model (rats with ligated L5/L6 spinal nerves). 19 However, the mechanisms of the analgesic effects of resveratrol against neuropathic pain have not been clearly demonstrated. By extending the above observations, one could speculate that the targeted delivery of a therapeutic activator of Sirt1 may result in antinociception at the spinal level.…”

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confidence: 99%

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Resveratrol Facilitates Pain Attenuation in a Rat Model of Neuropathic Pain Through the Activation of Spinal Sirt1

Yin

Zhao

et al. 2013

Regional Anesthesia and Pain Medicine

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Section: Discussionsupporting

confidence: 87%

Section: Lumbosacral Intrathecal Surgery and Drug Injectionmentioning

confidence: 99%

mentioning

confidence: 99%

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Resveratrol Facilitates Pain Attenuation in a Rat Model of Neuropathic Pain Through the Activation of Spinal Sirt1

Yin

Zhao

et al. 2013

Regional Anesthesia and Pain Medicine

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“…It was found in our study that an intrathecal administration of 5 µl 90 mM resveratrol [21], [30], [31] 1 h before CCI surgery, but not 45 mM, delayed the onset of thermal hyperalgesia (Fig. 5A) and mechanical allodynia (Fig.…”

Section: Resultsmentioning

confidence: 86%

“…The doses of NAD, resveratrol and EX-527 were selected according to previous reports and our preliminary experiments. Specifically, 400 mM and 800 mM NAD was prepared in saline [18], [28], [29], 45 mM and 90 mM resveratrol was dissolved in 20% DMSO [21], [30], [31] and 1.2 mM EX-527 was prepared in 20% DMSO [24]. The dosing schedule of drugs or vehicles was present in the Results or Figures.…”

Section: Methodsmentioning

confidence: 99%

Spinal SIRT1 Activation Attenuates Neuropathic Pain in Mice

et al. 2014

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Abnormal histone acetylation occurs during neuropathic pain through an epigenetic mechanism. Silent information regulator 1 (sir2 or SIRT1), a NAD-dependent deacetylase, plays complex systemic roles in a variety of processes through deacetylating acetylated histone and other specific substrates. But the role of SIRT1 in neuropathic pain is not well established yet. The present study was intended to detect SIRT1 content and activity, nicotinamide (NAM) and nicotinamide adenine dinucleotide (NAD) in the spinal cord using immunoblotting or mass spectroscopy over time in mice following chronic constriction injury (CCI) or sham surgery. In addition, the effect of intrathecal injection of NAD or resveratrol on thermal hyperalgesia and mechanical allodynia was evaluated in CCI mice. Finally, we investigated whether SIRT1 inhibitor EX-527 could reverse the anti-nociceptive effect of NAD or resveratrol. It was found that spinal SIRT1 expression, deacetylase activity and NAD/NAM decreased significantly 1, 3, 7, 14 and 21 days after CCI surgery as compared with sham group. In addition, daily intrathecal injection of 5 µl 800 mM NAD 1 h before and 1 day after CCI surgery or single intrathecal injection of 5 µl 90 mM resveratrol 1 h before CCI surgery produced a transient inhibitory effect on thermal hyperalgesia and mechanical allodynia in CCI mice. Finally, an intrathecal injection of 5 µl 1.2 mM EX-527 1 h before NAD or resveratrol administration reversed the anti-nociceptive effect of NAD or resveratrol. These data indicate that the reduction in SIRT1 deacetylase activity may be a factor contributing to the development of neuropathic pain in CCI mice. Our findings suggest that the enhancement of spinal NAD/NAM and/or SIRT1 activity may be a potentially promising strategy for the prevention or treatment of neuropathic pain.

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Synergistic interaction of diclofenac, benfotiamine, and resveratrol in experimental acute pain

Montiel-Ruiz

Granados‐Soto

García‐Jiménez

et al. 2011

Drug Development Research

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This study was designed to evaluate the possible synergistic antinociceptive interactions between diclofenac, benfotiamine, and resveratrol on acetic acid-induced nociception in mice. Isobolographic analyses were used to define the nature of the interactions between drugs. Diclofenac, benfotiamine, or resveratrol, as well as their combinations, produced a dose-dependent antinociceptive effect. ED 30 values were estimated for the individual drugs and isobolograms were constructed. Theoretical ED 30 values for the combinations estimated from the isobolograms were 170.9723.4, 4.971.0, and 173.3711.8 mg/kg for the diclofenac1benfotiamine, diclofenac1resveratrol, or benfotiamine1resveratrol combination, respectively. These values were significantly higher than the actually observed ED 30 values, which were 10.271.9, 0.370.1, and 5.370.8 mg/kg, respectively, indicating a synergistic interaction in the three combinations. Data indicate that low doses of the diclofenac1benfotiamine, diclofenac1resveratrol, or benfotiamine1resveratrol combination can interact synergistically to reverse acetic acid-induced nociception and they may represent a therapeutic advantage for clinical treatment of inflammatory pain. Drug Dev Res 72:397-404, 2011.

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Pharmacological evidence for the participation of NO–cyclic GMP–PKG–K+ channel pathway in the antiallodynic action of resveratrol

Cited by 37 publications

References 68 publications

Resveratrol Facilitates Pain Attenuation in a Rat Model of Neuropathic Pain Through the Activation of Spinal Sirt1

Resveratrol Facilitates Pain Attenuation in a Rat Model of Neuropathic Pain Through the Activation of Spinal Sirt1

Spinal SIRT1 Activation Attenuates Neuropathic Pain in Mice

Synergistic interaction of diclofenac, benfotiamine, and resveratrol in experimental acute pain

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