Pharmacological Evaluation of the Anticancer Activity of Extracts and Fractions of Lannea barteri Oliv. (Anacardiaceae) on Adherent Human Cancer Cell Lines

Abstract

In western Africa ethnomedicine, Lannea barteri Oliv. (Anacardiaceae) is believed to have activity against gastrointestinal, neurological and endocrine diseases. Previous studies on this plant have revealed antimicrobial, anticholinestrase, anticonvulsant, antioxidant and anti-inflammatory activities. However, the anticancer potential of L. barteri has not been studied to date. The aim of this study was to evaluate the anticancer potential of hot and cold extracts and silica gel column chromatographic fractions of L. barteri leaf and stem bark. The extracts and fractions were tested for anticancer activity by using the crystal violet cell proliferation assay on four adherent human carcinoma cell lines—5637 (bladder), KYSE 70 (oesophagus), SiSo (cervical) and HepG2 (hepatic). The inhibitory concentration (IC50) of fractions IH, 1I, 2E and 2F were: 3.75 ± 1.33, 3.88 ± 2.15, 0.53 ± 0.41, and 0.42 ± 0.45 µg/mL against KYSE 70 and 1.04 ± 0.94, 2.69 ± 1.17, 2.38 ± 3.64, 2.17 ± 1.92 µg/mL against SiSo cell lines respectively. Fraction 2E showed weak apoptotic activity at double the IC50 and some sign of cell cycle arrest in the G2/M phase. Thus, phytoconstituents of L. barteri leaf and stem bark can inhibit the proliferation of cancer cell lines indicating the presence of possible anticancer agents in this plant.

Enhancing Antibiotic Efficacy: Exploring Synergistic Interactions between Plant Extracts and Conventional Antibiotics

“…48 The stem bark and root of the L. barteri plant contain a variety of phytochemicals, including saponins, coumarins, polyphenols, alkaloids, tannins, quinones, steroids, terpenoids, and flavonoids. 49,50 Saponins, flavonoids, alkaloids, steroids, and glucosides are the main phytochemical components that have been identified in R. communis. The leaves of the plant have been shown to contain significant amounts of phenolic compounds such as gallic acid, quercetin, gentilic acid, rutin, epicatechin, and ellagic acid, as well as monoterpenoids (1, 8-cineole), camphor, and α-sesquiterpenoids (β-caryophyllene).…”

New C-13 Norisoprenoids and Flavonoids from <i>Lannea kerstingii</i>

“…The Anacardiaceae family, especially Lannea species are the site of multiple sources of secondary metabolites such as terpenes, polyphenol [1] [2] [3] [4] alkenyl phenols and alkenyl cyclohexenones [5], steroid [6] [4] and ceramide [7]. Lannea species have numerous pharmacological properties such as antibacterial [4] [5] [8], antoxidant [7] [8] [9], anticancer [10] [11] [12], antihypertensive [13].…”

A new flavanocoumarin glucoside from the roots of Lannea kerstingii

“…Pharmacological studies of some Lannea species have shown their anticancer, antibacterial and antioxidant activities (Sivaraj et al, 2018;Md. Hossain et al, 2018;Sabo et al, 2019;Mbaoji et al, 2020, Ombouma et al, 2022. The edible fruits of most of them are well known for their nutritional value in food or as a food supplement, and have enormous potential contribution in the economy and households (Atato et al, 2011;Sereme et al, 2014;Goudégnon et al, 2016;Diarra et al, 2016;Muhammad et al, 2018;Konaré et al, 2022).…”