Pharmacological evaluation of rat paw oedema induced byBothrops jararaca venom

Trebien, Herbert Arlindo; Calixto, João B.

doi:10.1007/bf01967293

Cited by 122 publications

(88 citation statements)

References 33 publications

(17 reference statements)

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“…Moreover, the fact that L-NAME, but not aminoguanidine, reduced venom-induced edema, strongly suggests that constitutive eNOS, but not iNOS, plays a key role in this phenomenon. Edema induced by Bothrops sp venoms is multifactorial, since a number of infl ammatory mediators are involved [14,43]. NO participates in the edematogenic effect induced by carrageenin in rats [44].…”

Section: Discussionmentioning

confidence: 99%

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

2006

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Abstract.Objective: To assess the role of nitric oxide in the most relevant local and systemic manifestations in mice injected with the venom of the snake Bothrops asper. Mice were pretreated with nitric oxide synthase inhibitors, and the modifi cations of the pathological effects induced by the venom were tested. Results: Inhibition of NO synthesis did not affect acute local myonecrosis and hemorrhage in muscle tissue upon intramuscular injection of venom. Local footpad edema was reduced in mice pretreated with the NO synthase inhibitor L-NAME, and a reduction in the extent of infl ammatory infi ltrate in muscle tissue was observed after envenomation in mice pretreated with L-NAME and aminoguanidine. The most pronounced effect of NOS inhibition by L-NAME was an increment in the lethal activity of the venom, when injected by the intraperitoneal route. Conclusion: Nitric oxide does not seem to play a signifi cant role in the local acute pathological alterations (hemorrhage and myonecrosis) induced by B. asper venom in mice, although it contributes to edema and infl ammatory infi ltrate. Nitric oxide exerts a protective role in the systemic pathophysiological manifestations leading to lethality.

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Section: Discussionmentioning

confidence: 99%

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

2006

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“…Such doses have been validated and used in previous pharmacological stud- (8)(9)(10). It has been suggested that the observed poor neutralization is based on the fact that hyperalgesia and edema develop at an extremely rapid rate after venom injection, associated with the release of endogenous mediators involved in these pharmacological effects (15,16,25,26). Thus, by the time antivenom antibodies reach the affected tissues, it is likely that the cascade of inflammatory events associated with hyperalgesia and edema has already started.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venoms

Picolo

Chacur

Gutiérrez

et al. 2002

Braz J Med Biol Res

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Neutralization of hyperalgesia induced by Bothrops jararaca and B. asper venoms was studied in rats using bothropic antivenom produced at Instituto Butantan (AVIB, 1 ml neutralizes 5 mg B. jararaca venom) and polyvalent antivenom produced at Instituto Clodomiro Picado (AVCP, 1 ml neutralizes 2.5 mg B. aspar venom). The intraplantar injection of B. jararaca and B. asper venoms caused hyperalgesia, which peaked 1 and 2 h after injection, respectively. Both venoms also induced edema with a similar time course. When neutralization assays involving the independent injection of venom and antivenom were performed, the hyperalgesia induced by B. jararaca venom was neutralized only when bothropic antivenom was administered iv 15 min before venom injection, whereas edema was neutralized when antivenom was injected 15 min or immediately before venom injection. On the other hand, polyvalent antivenom did not interfere with hyperalgesia or edema induced by B. asper venom, even when administered prior to envenomation. The lack of neutralization of hyperalgesia and edema induced by B. asper venom is not attributable to the absence of neutralizing antibodies in the antivenom, since neutralization was achieved in assays involving preincubation of venom and antivenom. Cross-neutralization of AVCP or AVIB against B. jararaca and B. asper venoms, respectively, was also evaluated. Only bothropic antivenom partially neutralized hyperalgesia induced by B. asper venom in preincubation experiments. The present data suggest that hyperalgesia and edema induced by Bothrops venoms are poorly neutralized by commercial antivenoms even when antibodies are administered immediately after envenomation. Correspondence

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“…The present study shows that subplantar injection of NhaV can cause a significant paw edema in rats. Envenoming also produced edema and altered vascular permeability in mouse hind paw (42,69). Following these responses, there was an abundant leukocyte infiltration and consequent hemorrhage due to the high doses of Dipyridamole is a potent inhibitor of platelet aggregation.…”

Section: Effects Of Nhav On Rat Paw Edemamentioning

confidence: 99%

Pharmacological characterization of rat paw edema induced by Naja haje arabica venom

Alasmari¹

2005

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:This investigation was performed in order to assess the inflammatory response induced by Naja haje arabica venom (NhaV) in rat hind paw. The Commercially available antivenom was ineffective when administered intravenously, whereas its local administration with NhaV attenuated the edema formation (P<0.001). In conclusion, NhaV-induced edema in rat paw involves PLA 2 and adenosine mechanisms. Additionally, the use of polyspecific antivenom, intravenously, was ineffective in preventing NhaV-induced edema.KEY WORDS: rat paw edema, Naja haje arabica venom, inflammatory mediators, inhibitor, antivenom.

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Pharmacological evaluation of rat paw oedema induced byBothrops jararaca venom

Cited by 122 publications

References 33 publications

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

Evaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venoms

Pharmacological characterization of rat paw edema induced by Naja haje arabica venom

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