Pharmacological (ethanol) and mutation (sam2 KO) induced impairment of novelty preference in zebrafish quantified using a new three-chamber social choice task

Ariyasiri, Krishan; Choi, Tae‐Ik; Kim, Oc-Hee; Hong, Ted; Kim, Cheol-Hee

doi:10.1016/j.pnpbp.2018.06.009

Cited by 20 publications

(29 citation statements)

References 56 publications

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“…Although we have observed a normal social preference in our oxtr ( −/− ) mutants, we did observe that an impaired oxytocinergic signalling affected social recognition, comprising a cognitive function that has been shown consistently to be affected by OXT in other species . It has already been reported that the zebrafish is able to discriminate between novel and familiar conspecifics, exhibiting social recognition memory, and, in the present study, for the first time, we have shown that this mechanism is regulated by OXT signalling, suggesting a conserved mechanism across species . Interestingly, a conditional deletion of OXTR in lateral septum of mice impairs social memory but not social approach .…”

Section: Discussionsupporting

confidence: 52%

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

Ribeiro

Nunes

Gliksberg

et al. 2020

J Neuroendocrinology

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| INTRODUC TI ONNonapeptides are known to be important players in the regulation of social behaviour across taxa. 1 They evolved from a common ancestral molecule, the arginine vasotocin, after a gene duplication event in early-jawed fish. 2 One copy originated the vasopressin-like peptides, which retained the most primitive functions of the ancestral molecule, namely the regulation of osmotic balance. The other originated the oxytocin (OXT)-like peptides, which have greatly diverged across species, being recruited for diverse homeostatic processes, including parturition and lactation in mammals. Throughout evolution, these nonapeptides have also been involved in the regulation of social behaviours, with vasopressin being more involved in aggression and agonistic behaviours and OXT in affiliative behaviours and sociality. 3 AbstractSociality is a complex phenomenon that involves the individual´s motivation to approach their conspecifics, along with social cognitive functions that enable individuals to interact and survive. The nonapeptide oxytocin (OXT) is known to regulate sociality in many species. However, the role of OXT in specific aspects of sociality is still not well understood. In the present study, we investigated the contribution of the OXT receptor (OXTR) signalling in two different aspects of zebrafish social behaviour: social preference, by measuring their motivation to approach a shoal of conspecifics, and social recognition, by measuring their ability to discriminate between a novel and familiar fish, using a mutant zebrafish lacking a functional OXTR. Although oxtr mutant zebrafish displayed normal attraction to a shoal of conspecifics, they exhibited reduced social recognition. We further investigated whether this effect would be social-domain specific by replacing conspecific fish by objects. Although no differences were observed in object approach, oxtr mutant fish also exhibited impaired object recognition. Our findings suggest that OXTR signalling regulates a more general memory recognition of familiar vs novel entities, not only in social but also in a non-social domain, in zebrafish. K E Y W O R D Sautism spectrum disorders, oxytocin, social preference, social recognition, zebrafish S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.

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Section: Discussionsupporting

confidence: 52%

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

Ribeiro

Nunes

Gliksberg

et al. 2020

J Neuroendocrinology

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“…We found test fish spending significantly more time very close to big stimulus (zone-5) compared with the smaller stimulus conspecific (zone-1) ones. Zone transitions in a study by Ariyasiri et al 9 also reflected this preference: we found a significantly higher number of transitions between zone-5 and zone-4 (closer to the large stimulus fish) than between zone-1 and zone-2 (closer to the smaller stimulus fish).…”

Section: Discussionsupporting

confidence: 80%

“…For the analysis, the center test chamber was divided into five zones ( Fig. 1B) as previously described, 9 namely, zone-1, very close to smaller stimulus fish; zone-2, close to smaller stimulus fish; zone-3, center; zone-4, close to big stimulus fish; and zone-5, very close to big stimulus fish.…”

Section: Behavioral Apparatusmentioning

confidence: 99%

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The Body Size of Stimulus Conspecifics Affects Social Preference in a Binary Choice Task in Wild-Type, But Not in dyrk1aa Mutant, Zebrafish

et al. 2019

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The zebrafish has become an appropriate animal model in the analysis of numerous human brain disorders. A variety of neuropsychiatric conditions and neurodevelopmental disorders are comorbid with abnormal social behavior. Given the translational relevance of zebrafish, multidisciplinary studies employing behavioral, neurobiological, and molecular methods with this species may provide insights into human central nervous system (CNS) disorders. Many of these studies impinge upon our ability to properly induce and quantify the behavior of zebrafish, a relatively understudied aspect of this species. In this study, we investigate how the body size of conspecifics relative to that of the test subject influences social (shoaling) responses in zebrafish. We found a robust preference by wild-type (WT) test zebrafish toward big conspecifics, but not toward smaller conspecifics. Additionally, we tested an autism-relevant zebrafish knockout (KO) model. The dyrk1aa KO zebrafish showed impaired social preference compared with WT in the social behavior test. Our results confirm the effect of relative body size on social preference and that the social preference task developed for zebrafish may uncover the function of genes and biological mechanisms potentially associated with human CNS disorders.

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“…Time spent near conspecifics, speed, and distance traveled are all measured to evaluate social behavior in fish, similar to social interaction tests in rodents (Norton et al, 2019). 3-Chamber social choice test: Similar to the visually mediated social preference test, the three chamber social choice test uses a center chamber, and a chamber on either side; one containing fish that were acclimated with the controls and the other chamber is originally left empty during acclimation, and naive fish are placed in the chamber during phase two (Ariyasiri et al, 2019). This test is also performed on adult fish.…”

Section: Box 1 | Behavioral Tests For Zebrafish Researchmentioning

confidence: 99%

Using Zebrafish to Model Autism Spectrum Disorder: A Comparison of ASD Risk Genes Between Zebrafish and Their Mammalian Counterparts

Rea

Raay

2020

Front. Mol. Neurosci.

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Autism spectrum disorders (ASDs) are a highly variable and complex set of neurological disorders that alter neurodevelopment and cognitive function, which usually presents with social and learning impairments accompanied with other comorbid symptoms like hypersensitivity or hyposensitivity, or repetitive behaviors. Autism can be caused by genetic and/or environmental factors and unraveling the etiology of ASD has proven challenging, especially given that different genetic mutations can cause both similar and different phenotypes that all fall within the autism spectrum. Furthermore, the list of ASD risk genes is ever increasing making it difficult to synthesize a common theme. The use of rodent models to enhance ASD research is invaluable and is beginning to unravel the underlying molecular mechanisms of this disease. Recently, zebrafish have been recognized as a useful model of neurodevelopmental disorders with regards to genetics, pharmacology and behavior and one of the main foundations supporting autism research (SFARI) recently identified 12 ASD risk genes with validated zebrafish mutant models. Here, we describe what is known about those 12 ASD risk genes in human, mice and zebrafish to better facilitate this research. We also describe several non-genetic models including pharmacological and gnotobiotic models that are used in zebrafish to study ASD.

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Pharmacological (ethanol) and mutation (sam2 KO) induced impairment of novelty preference in zebrafish quantified using a new three-chamber social choice task

Cited by 20 publications

References 56 publications

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

The Body Size of Stimulus Conspecifics Affects Social Preference in a Binary Choice Task in Wild-Type, But Not in dyrk1aa Mutant, Zebrafish

Using Zebrafish to Model Autism Spectrum Disorder: A Comparison of ASD Risk Genes Between Zebrafish and Their Mammalian Counterparts

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