Pharmacological Effects of Peganum Harmala L. Root Extract on Isolated Rat small Intestine

Shatarat, Amjad; Abuhamdah, Sawsan; Al-Essa, Mohamed K.; Mohammed, Falah Saleh; Al-Olimat, Suleiman

doi:10.5530/pc.2014.3.6

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…It has been reported that Peganum harmala exerts broad pharmacological effects such as amelioration of cardiovascular, gastrointestinal and respiratory problems, as well as possessing antioxidant, antimicrobial, antiviral, antihelmintic, antifungal, antineoplastic properties and pain-relieving potential [5,6]. In previous studies, the anti-spasmogenic activities of ethanolic extract of P. harmala and its alkaloids on intestinal SMCs, were demonstrated [7,8].…”

Section: Introductionmentioning

confidence: 99%

Alkaloids from <i>Peganum harmala</i> attenuated contractile responses of vascular smooth muscle cells

Al-Essa,

Alefishat,

AbuHamdah

et al. 2023

Trop. J. Pharm Res

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Purpose: To investigate the contractile responses of vascular smooth muscle cells (VSMCs) to spasmogens after incubation with harmaline, harmine, and harmalol, which are alkaloids obtained from Peganum harmala L., a member of the Zygophyllaceae family. Methods: Contractile responses of VSMCs to norepinephrine (NE; 1 µmol/L) and potassium chloride (KCl; 60 mmol/L) were recorded in rat aortic ring preparations pre-incubated with 0.5, 1, 5 and 10 µmol/L of each alkaloid for 15 min. Responses were expressed as mean values of contractions in incubated preparations, relative to the recorded tension prior to treatment with alkaloids. Results: Pre- incubation with harmaline at concentration of 10 µmol/L significantly reduced contractile responses to NE by 69.0 ± 3.0 % (p < 0.00002), and decreased KCl-induced contraction by 34.0 ± 9.0 % (p < 0.05). Harmalol was the most effective in inhibiting contractions to KCl (48.0 ± 9.0 %, p < 0.01). However, harmalol produced relatively moderate inhibitory effects on NE-induced contractions (46.0 ± 4.0 %, p < 0.005), followed by harmine (52.0 ± 8.0 %, p < 0.02), but it did not significantly affect contractile responses to KCl. Conclusion: These results highlight the differential effects of pre-incubation with alkaloids from P. harmala and their potential effects on the prevention of VSMC spasms induced by either chemicals or stimuli that change the membrane potential.

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Section: Introductionmentioning

confidence: 99%

Alkaloids from <i>Peganum harmala</i> attenuated contractile responses of vascular smooth muscle cells

Al-Essa,

Alefishat,

AbuHamdah

et al. 2023

Trop. J. Pharm Res

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The efficacy of biosynthesized silver nanoparticles against Pseudomonas aeruginosa isolates from cystic fibrosis patients

Al-Momani,

Almasri,

Al Balawi

et al. 2023

Sci Rep

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The high antibiotic resistance of Pseudomonas aeruginosa (PA) makes it critical to develop alternative antimicrobial agents that are effective and affordable. One of the many applications of silver nanoparticles (Ag NPs) is their use as an antimicrobial agent against bacteria resistant to common antibiotics. The key purpose of this research was to assess the antibacterial and antibiofilm effectiveness of biosynthesized Ag NPs against six biofilm-forming clinically isolated strains of PA and one reference strain (ATCC 27853). Ag NPs were biosynthesized using a seed extract of Peganum harmala as a reducing agent. Ag NPs were characterized by Ultraviolet–visible (UV–Vis) spectroscopy and scanning transmission electron microscopy (STEM). The effect of Ag NPs on biofilm formation and eradication was examined through micro-titer plate assays, and the minimal inhibitory (MIC) and minimum bactericidal (MBC) concentrations determined. In addition, real-time polymerase chain reactions (RT-PCR) were performed to examine the effects of Ag NPs on the expression of seven PA biofilm-encoding genes (LasR, LasI, LssB, rhIR, rhII, pqsA and pqsR). The biosynthesized Ag NPs were spherically-shaped with a mean diameter of 11 nm. The MIC for each PA strain was 15.6 µg/ml, while the MBC was 31.25 µg/ml. All PA strains exposed to Ag NPs at sub-inhibitory concentrations (0.22–7.5 µg/ml) showed significant inhibitory effects on growth and biofilm formation. Biomass and biofilm metabolism were reduced dependent on Ag NP concentration. The expression of the quorum-sensing genes of all strains were significantly reduced at an Ag NP concentration of 7.5 µg/ml. The results demonstrate the extensive in-vitro antibacterial and antibiofilm performance of Ag NPs and their potential in the treatment of PA infection. It is recommended that future studies examine the possible synergy between Ag NPs and antibiotics.

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Improving health benefits with considering traditional and modern health benefits of Peganum harmala

2021

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Background Medicinal plants are potential source of natural products that play an important role in preventing different human diseases. P. harmala is used traditionally as emmenagogue and an abortifacient agent in the Middle East, North of Africa and West of China. It belongs to the family of Zygophyllaceae and it is a wild growing flowering plant. Its seeds are main medicinal part of the plant. Methods The current searching was done by the keywords in main indexing systems including PubMed/MEDLINE, Scopus, and Institute for Scientific Information Web of Science as well as the search engine of Google Scholar. The keywords were wild rue, traditional medicine, Harman, health benefits, and pharmaceutical science. Results The most important uses of P. harmala in traditional pharmaceutical sciences are in cardiovascular, gasterointestinal, nervous, endocrine, neoplasm and tumors, pain relieving, organisms, diabetes, respiratory, disinfectant, anti-pyretic, skin and hair, rheumatism, arthritis and inflammation, and ulcers. Pharmacological effects of P. harmala are in cardiovascular system, nervous system, antimicrobial effects, antineoplasm, nervous system, endocrine, gastrointestinal effects, osteocytes, endocrine and respiratory system. Phenolic compounds are the main reason of antioxidant capacity. Conclusions Due to its pharmacological activities, P. harmala is a high potential medicinal herb and the suggestion is to increases by doing research in efficacy and safety.

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Pharmacological Effects of Peganum Harmala L. Root Extract on Isolated Rat small Intestine

Cited by 3 publications

References 27 publications

Alkaloids from <i>Peganum harmala</i> attenuated contractile responses of vascular smooth muscle cells

Alkaloids from <i>Peganum harmala</i> attenuated contractile responses of vascular smooth muscle cells

The efficacy of biosynthesized silver nanoparticles against Pseudomonas aeruginosa isolates from cystic fibrosis patients

Improving health benefits with considering traditional and modern health benefits of Peganum harmala

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