Pharmacological effects of Lavandula officinalis Chaix and its polyphenols: Focus on their in vivo estrogenic and anti-inflammatory properties

Slighoua, Meryem; Mahdi, Ismail; Amrati, Fatima Ez-Zahra; Boucetta, Nabil; Cristo, Francesca Di; Boukhira, Smahane; Hamsas, Amal El youbi el; Tattou, Mohamed Ibn; Grafov, Andriy; Bari, Amina; Bousta, Dalila

doi:10.1016/j.sajb.2021.11.014

Cited by 7 publications

(11 citation statements)

References 38 publications

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“…Apigenin, caffeic acid, ferulic acid, catechin, myricetin, and gallic acid have already been identified in L. officinalis Chaix [ 33 , 34 , 35 ]. Several previous studies have proven the antioxidant, anti-inflammatory, analgesic, and antibacterial effects of these compounds [ 36 , 37 , 38 , 39 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

The LC-MS/MS Identification and Analgesic and Wound Healing Activities of Lavandula officinalis Chaix: In Vivo and In Silico Approaches

Slighoua

Chebaibi

Mahdi

et al. 2022

Plants

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We earlier emphasized in vivo the lavender plant’s (Lavandula officinalis Chaix.) anti-inflammatory and estrogenic activities and described the chemical compositions of its hydro-ethanolic (HE) extract. We used LC-MS/MS and GC-MS analyses to profile the phytochemical composition of the HE extract and to assess the analgesic and wound-healing effects of both the hydro-ethanolic (HE) and polyphenolic (LOP) extracts in vivo and in silico. The analgesic activity was studied using two methods: acetic acid and formalin injections in mice. The wound-healing activity was carried out over 25 days using a burn model in rats. In the in silico study, the polyphenols identified in the plant were docked in the active sites of three enzymes: casein kinase-1, cyclooxygenase-2, and glycogen synthase kinase-3β. The LC-MS/MS identified some phenolic compounds, mainly apigenin, catechin, and myricetin, and the GC-MS analysis revealed the presence of 19 volatile compounds with triazole, D-glucose, hydroxyphenyl, and D-Ribofuranose as the major compounds. The HE and LOP extracts showed significant decreases in abdominal writhes, and the higher licking time of the paw (57.67%) was observed using the LOP extract at 200 mg/kg. Moreover, both extracts showed high healing percentages, i.e., 99.31 and 92.88%, compared to the control groups, respectively. The molecular docking showed that myricetin, amentoflavone, apigenin, and catechin are the most active molecules against the three enzyme receptors. This study sheds light on the potential of L. officinalis Chaix as a source of natural products for pharmaceutical applications for analgesic purposes as well as their utility in promoting burn-healing activity.

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Section: Discussionmentioning

confidence: 99%

The LC-MS/MS Identification and Analgesic and Wound Healing Activities of Lavandula officinalis Chaix: In Vivo and In Silico Approaches

Slighoua

Chebaibi

Mahdi

et al. 2022

Plants

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“…Next, the aqueous phase was extracted in ethyl acetate (200 mL × 3) which was evaporated later. Using 300 mL of distilled water, the residue was dissolved and then lyophilized [ 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats

et al. 2023

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Parsley (Petroselinum sativum Hoffm.) is renowned for its ethnomedicinal uses including managing pain, wound, and dermal diseases. We previously highlighted the estrogenic and anti-inflammatory properties of parsley and profiled the phytochemistry of its polyphenolic fraction using HPLC-DAD. To extend our investigation, we here characterized the phytochemical composition of the hydro-ethanolic extract using LC-MS/MS and GC-MS upon silylation, and evaluated the antioxidant, analgesic, antimicrobial, and wound healing activities of its hydro-ethanolic and polyphenolic fraction. The antioxidant property was assessed using FRAP, DPPH, and TAC assays. The antimicrobial activity was tested against four wound infectious microbes (Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans). The analgesic effect was studied using acetic acid (counting the number of writhes) and formalin (recording the licking and biting times) injections while the wound healing activity was evaluated using burn model in vivo. The LC-MS/MS showed that the hydro-ethanolic contains four polyphenols (oleuropein, arbutin, myricetin, and naringin) while GC-MS revealed that it contains 20 compounds including malic acid, D-glucose, and galactofuranoside. The hydro-ethanolic (1000 mg/kg) decreased abdominal writhes (38.96%) and licking time (37.34%). It also elicited a strong antioxidant activity using DPPH method (IC50 = 19.38 ± 0.15 µg/mL). Polyphenols exhibited a good antimicrobial effect (MIC = 3.125–12.5 mg/mL). Moreover, both extracts showed high wound contraction by 97.17% and 94.98%, respectively. This study provides evidence that P. sativum could serve as a source of bio-compounds exhibiting analgesic effect and their promising application in mitigating ROS-related disorders, impeding wound infections, and enhancing burn healing.

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“…This field of study is known as herbal gynecology, and it has gained popularity in recent years. Many plants have been identified as having potential therapeutic effects for female infertility, such as Senecio biafrae , Petroselinum sativum , and Lavandula officinalis [ 10 , 11 , 12 ]. Indeed, it has long been known that a wide range of exogenous substances such as xenoestrogens, phytoestrogens, and steroidal estrogens can mimic the activities of endogenous estrogen to different degrees [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous studies, we investigated the estrogenic effect of Petroselinum sativum [ 12 ] and Lavandula officinalis [ 11 ] extracts and profiled their phytochemical constituents using HPLC. Interestingly, these studies demonstrated that ferulic acid and quercetin had the highest concentration among the identified molecules.…”

Section: Introductionmentioning

confidence: 99%

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Quercetin and Ferulic Acid Elicit Estrogenic Activities In Vivo and In Silico

et al. 2023

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In this study, we examined the sub-acute toxicity of quercetin and ferulic acid and evaluated their effects on protein, cholesterol, and estrogen levels in vivo. Six groups of female Wistar rats were fed by gavage. The first and second groups represent the positive (Clomiphene citrate 10 mg/kg) and negative (NaCl 0.9%) control groups, while the other groups received quercetin and ferulic acid at doses of 5 and 10 mg/kg/day for 28 days. The sub-acute toxicity was monitored by examining the weights, biochemical parameters (AST, ALT, ALP, urea, and CREA), and histological changes in the kidneys and liver of the treated animals. Furthermore, the in vivo estrogenic effects were studied in terms of the serum and ovarian cholesterol levels, serum estradiol, and uterine proteins. Finally, Docking studies were conducted to evaluate the binding affinity of quercetin and ferulic acid for alpha and beta estrogen receptors. Results showed that both compounds were devoid of any signs of nephrotoxicity or hepatotoxicity. Additionally, quercetin and ferulic acid caused significant estrogenic effects evidenced by an increase of 8.7 to 22.48% in serum estradiol, though to a lesser amount than in the reference drug-treated group (64.21%). Moreover, the two compounds decreased the serum cholesterol levels (12.26–32.75%) as well as the ovarian cholesterol level (11.9% to 41.50%) compared to the negative control. The molecular docking in estrogen alpha and estrogen beta active sites showed high affinity of quercetin (−10.444 kcal/mol for estrogen alpha and −10.662 kcal/mol for estrogen beta) and ferulic acid (−6.377 kcal/mol for estrogen alpha and −6.3 kcal/mol for estrogen beta) to these receptors. This study provides promising insights into the potential use of quercetin as a therapeutic agent for the management of female fertility issues.

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Pharmacological effects of Lavandula officinalis Chaix and its polyphenols: Focus on their in vivo estrogenic and anti-inflammatory properties

Cited by 7 publications

References 38 publications

The LC-MS/MS Identification and Analgesic and Wound Healing Activities of Lavandula officinalis Chaix: In Vivo and In Silico Approaches

The LC-MS/MS Identification and Analgesic and Wound Healing Activities of Lavandula officinalis Chaix: In Vivo and In Silico Approaches

LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats

Quercetin and Ferulic Acid Elicit Estrogenic Activities In Vivo and In Silico

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