Pharmacological Effects of Adenosine on Canine Penile Erection.

Takahashi, Yoshiastu; Ishii, Nobuhisa; Lue, Tom F.; Tanagho, Emil A.

doi:10.1620/tjem.165.49

Cited by 20 publications

(21 citation statements)

References 12 publications

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“…This effect, which was unaffected by atropine and hexamethonium, could be obtained with no changes in systemic blood pressure. In addition, adenosine produced full erection on intracavernosal administration [25].…”

Section: Discussionmentioning

confidence: 92%

Effect of hypothyroidism on the purinergic responses of corpus cavernosal smooth muscle in rabbits

et al. 2008

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Section: Discussionmentioning

confidence: 92%

Effect of hypothyroidism on the purinergic responses of corpus cavernosal smooth muscle in rabbits

et al. 2008

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“…Previous reports demonstrated that intracavernous injection of adenosine induces full erection in dogs (Takahashi et al, 1992) and that the erectile response induced by cavernous nerve stimulation is enhanced by the adenosine uptake inhibitor dipyridamole (Takahashi et al, 1991). In the rabbit corpus cavernosum, adenosine has potent relaxant activity, acting through the A2 A receptor subtype (Mantelli et al, 1995) and it also modulates both adrenergic and NANC neurotransmission (Chiang et al, 1994).…”

Section: ϫ8mentioning

confidence: 99%

Determination of Adenosine Effects and Adenosine Receptors in Murine Corpus Cavernosum

Tostes¹,

Giachini²,

Carneiro³

et al. 2007

J Pharmacol Exp Ther

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This study tested the hypothesis that adenosine, in murine corpora cavernosa, produces direct relaxation of smooth muscle cells and inhibition of contractile responses mediated by sympathetic nerve stimulation. Penes were excised from anesthetized male C57BL/6 mice, dissected, and cavernosal strips were mounted to record isometric force. Adenosine, 2-chloroadenosine (stable analog of adenosine), and 2-phenylaminoadenosine (CV1808) (A2 A /A2 B agonist) produced concentrationdependent relaxations of phenylephrine-contracted tissues. Relaxation to 2-chloroadenosine was inhibited, in a concentrationdependent manner, by 2-(2-furanyl)-7-(2-phenylethyl)-7H-pyrazolo [4,3-e] Ϫ8-10 Ϫ6 M). The combination of both antagonists abrogated 2-chloroadenosine-induced relaxation. Electrical field stimulation (EFS; 1-32 Hz) of adrenergic nerves produced frequency-dependent contractions that were inhibited by compounds that increase adenosine levels, such as 5Ј-iodotubercidin (adenosine kinase inhibitor), erythro-9-(2-hydroxy-3-nonyl)adenine (adenosine deaminase inhibitor), and dipyridamole (inhibitor of adenosine transport). The adenosine A1 receptor agonist N 6 -cyclopentyladenosine (C8031) right-shifted contractile responses to EFS, with a significant inhibitory effect at 10 Ϫ6 M. Blockade of adenosine A1 receptors with 8-cyclopentyl-1,3-dipropylxanthine (C101) (10 Ϫ7 M) enhanced contractile responses to EFS and eliminated the inhibitory effects of 5Ј-iodotubercidin. Dipyridamole and 5Ј-iodotubercidin had no effect on adenosine-mediated relaxation. In summary, adenosine directly relaxes cavernosal smooth muscle cells, by the activation of A2 A / A2 B receptor subtypes. In addition, adenosine negatively modulates sympathetic neurotransmission, by A1 receptor subtype activation, in murine corpora cavernosa. Adenosine may subserve dual roles in modulating the physiological mechanisms of erection in mice.The balance between contracting and relaxing factors controls smooth muscle tone of both the penile vasculature and corpora cavernosa, and, therefore, it determines the functional state of the penis: flaccidity or erection (Andersson, 2001;Leite et al., 2007). Efferent autonomic sympathetic and parasympathetic nerves regulate penile function, via release of norepinephrine and acetylcholine, respectively. In addition, many investigators have provided evidence for functional roles of nonadrenergic-noncholinergic (NANC) inhibitory and excitatory nerves, which contain not only neuropeptides, such as neuropeptide Y, vasoactive intestinal polypeptide, substance P, and calcitonin gene-related polypeptide, but also transmitters such as nitric oxide (NO) and adenosine triphosphate (ATP) (Giuliano and Rampin,

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“…Intracavernosal injection of adenosine induced a full erection in cats [394,395]. Adenosine also had prejunctional inhibitory actions on excitatory sympathetic cotransmission to the rabbit corpus cavernosum, probably acting via A 2B receptors [79].…”

Section: Penis and Penile Erectionmentioning

confidence: 99%

Purinergic signalling in the reproductive system in health and disease

Burnstock

2013

Purinergic Signalling

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There are multiple roles for purinergic signalling in both male and female reproductive organs. ATP, released as a cotransmitter with noradrenaline from sympathetic nerves, contracts smooth muscle via P2X1 receptors in vas deferens, seminal vesicles, prostate and uterus, as well as in blood vessels. Male infertility occurs in P2X1 receptor knockout mice. Both short-and long-term trophic purinergic signalling occurs in reproductive organs. Purinergic signalling is involved in hormone secretion, penile erection, sperm motility and capacitation, and mucous production. Changes in purinoceptor expression occur in pathophysiological conditions, including pre-eclampsia, cancer and pain.

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Pharmacological Effects of Adenosine on Canine Penile Erection.

Cited by 20 publications

References 12 publications

Effect of hypothyroidism on the purinergic responses of corpus cavernosal smooth muscle in rabbits

Effect of hypothyroidism on the purinergic responses of corpus cavernosal smooth muscle in rabbits

Determination of Adenosine Effects and Adenosine Receptors in Murine Corpus Cavernosum

Purinergic signalling in the reproductive system in health and disease

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