Pharmacological effects of active saponins from Panax ginseng Meyer

Abstract

Purpose: To investigate the pharmacological effects of the active saponins isolated from Panax ginseng Meyer (P. ginseng) via extraction, heat treatment, and enzyme conversion.
 Methods: The effects of active saponins on rat blood were determined using a multichannel analyzer. The population doubling time (PDT) of mesenchymal stem cells (MSCs) and human-derived leukocyte cancer cells (A549) was determined by cell counting. b-galactosidase was measured in human toothderived stem cells (HTS) using a β-galactosidase ELISA kit.
 Results: Intraperitoneal administration of active saponins resulted in 30.09 % increase in red blood cell count and 55.55 % decrease in blood triglyceride concentrations. The stimulatory effect of active saponins (10 ng/mL) on cellular differentiation was determined based on PDT of MSCs, which decreased by 33.82 % compared to control. A 22.29 % increase in PDT of A549 cells demonstrated the suppressive effects of active saponins on cancer cell growth. Active saponins (10 ng/mL) also decreased intracellular β-galactosidase concentration by 20.42 % in HTS cells.
 Conclusion: Administration of active saponins to rats extends the lifespan, promotes differentiation in MSCs, suppresses A549 cell differentiation, and reduces TG and b-galactosidase associated with aging in HTS. Thus, active saponins have potentially beneficial effects in humans.

Inhibition of Phenylhydrazine-induced Hematotoxicity by Bioconverted Ginsenosides

“…Previous studies have shown that Rg2 and Rh1 have erythrocyte protective effects, but no reports on compound K have been reported. 25 The compound K concentration was based on 20 nM, referring to a previous study by Bae et al 22 A comparison of the packed blood sample with compound K and non-treatment groups showed that the half-life was 30 days in the untreated group, but the half-life extended to 45 days in the treated group (data not shown). A microscopic examination of the erythrocyte morphology revealed that the compound K group significantly reduced the occurrence of debris and aggregation.…”

“…Compound K was treated at a final concentration of 20 nM, as previously described. 22 To compare the erythrocyte half-life span, a CBC analyzer was used. The control (30 days, from 5.07 to 1.94 × 10 6 cells/μL), compound K (30 days, from 5.39 to 4.1 × 10 6 cells/μL), and the compound K-treated blood groups had an erythrocyte half-life-span of 15 days longer.…”

Effect of <i>Angelica gigas</i> Nakai extract on hepatic damage in rats

“…D/DA ethanol solution was firstly mixed with the DPPH stock solution in methanol for DPPH radical scavenging activity. The final concentration was 0.2 mM for DPPH in every D/DA (1,10,20,40,80, and 160 µg/mL) ethanol solution (experiment), 0.2 mM DPPH in ethanol (Control), and D/DA in a methanol and ethanol solutions (blank). The standard solution used was αtocopherol.…”

“…Angelica gigas Nakai is a medicinal herb that is widely used in Asia (Korea, Japan, and China) [1]. In the US and Europe, AGN is used as a dietary supplement [2].…”