Pharmacological differences of non-adrenergic inhibitory response and of ATP-induced relaxation in guinea-pig tracheal strip-chains

Kamikawa, Yuichiro; Shimo, Yasuo

doi:10.1111/j.2042-7158.1976.tb04077.x

Cited by 35 publications

(18 citation statements)

References 6 publications

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“…Evidence for more than one type of purine receptor was presented for iso-PURINERGIC SIGNALING IN THE AIRWAYS lated guinea pig trachea, mediating relaxation with a potency order of adenosine Ͼ 5Ј-AMP Ͼ ATP Ͼ ADP (Jones et al, 1980). Excitatory junctional potentials and contraction, which were reported to be blocked by atropine, were recorded in guinea pig tracheal smooth muscle in response to nerve stimulation, followed by prolonged relaxations that were blocked by indomethacin, suggesting mediation by prostaglandins (Kamikawa and Shimo, 1976). Later, EP 2 prostaglandin receptors were shown to mediate tracheal relaxation to ATP (Fortner et al, 2001).…”

Section: B Smooth Muscle and Its Innervationmentioning

confidence: 99%

Purinergic Signaling in the Airways

Burnstock

Brouns²,

Adriaensen³

et al. 2012

Pharmacol Rev

173

158

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Section: B Smooth Muscle and Its Innervationmentioning

confidence: 99%

Purinergic Signaling in the Airways

Burnstock

Brouns²,

Adriaensen³

et al. 2012

Pharmacol Rev

173

158

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“…After adjusting the applied tension, atropine (1 pM) and guanethidine (10puM) were added to the KR in all treatment groups in order to obtain cholinergic blockade (Andersson et al, 1981;Lundberg et al, 1983;Taylor et al, 1984) and adrenergic depletion (Kamikawa & Shimo, 1976;Grundstrom et al, 1981;Brock et al, 1988). In the resting preparations, three different levels of active tension (resting tone) were established by the pharmacological manipulation of the spontaneous airway tone, by use either of the cyclo-oxygenase inhibitor, indomethacin (10pM), or the leukotriene receptor antagonist FPL 55712 (11.5pM) or the vehicle (Linden et al, 1991b).…”

Section: Experimental Designmentioning

confidence: 99%

Non‐adrenergic, non‐cholinergic neural activation stabilizes smooth‐muscle tone independently of eicosanoid factors in guinea‐pig isolated airways

Lindén

Ullman

Skoogh

1991

British J Pharmacology

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1 We examined the effect of variations in resting smooth-muscle tone induced by eicosanoid inhibitors on the direction and magnitude of the non-adrenergic, non-cholinergic (NANC) nervous response in guinea-pig isolated airways. 2 NANC responses (in the presence of 1 gM atropine, 1IOM guanethidine) to electrical field stimulation (1200 mA; 0.5 ms; 240s; 3 Hz) were studied in guinea-pig isolated airway preparations (n = 4-7) taken from the proximal trachea, the distal trachea and the main bronchus. Tissues were treated with either the cyclo-oxygenase-blocker, indomethacin (10pM), or the leukotriene receptor antagonist, FPL 55712 (11.5 gM), to modulate the resting tone.3 Depending on the level of resting tone prior to electrical field stimulation, NANC activation induced either a contraction or a relaxation converging towards a similar level of tone, a 'tonus equilibrium'. This tonus equilibrium displayed an increasing level towards the periphery (3 + 3 (s.e.mean) % for the proximal trachea, 28 + 3% for the distal trachea and 54 + 4% for the main bronchus, in % of maximum active tension). After NANC activation, the tonus returned to a level similar to the resting tone. 4 We conclude that changes in resting smooth-muscle tone not only affect the magnitude but also the direction of the NANC response. It is suggested that the NANC nervous system is a stabilizing factor in the regulation of airway smooth-muscle tone, independent of eicosanoid factors. An increasing contractile component and a decreasing relaxant component of the NANC system towards the peripheral airways is indicated.

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“…It seems less likely that the transmitter responsible for the relaxant effect was a purine nucleotide since the response on nerve stimulation was left completely unaffected by both quinidine and theophylline. This conclusion was also favored by Kamikawa & Shimo (1976) who found that the inhibitory response in guinea pig trachea was left unaffected by the prostaglandine inhibitors indomethacine and polyphloretine phosphate, whereas the relaxant effect of ATP was blocked by these inhibitors. However, recent findings by immunohistochemistry has revealed that both substance P (Nilsson et al 1977) and VIP (vasoactive intestinal polypeptide) (Uddman et a/.…”

Section: Discussionmentioning

confidence: 90%

Pharmacological Characterization of the Autonomous Innervation of the Guinea Pig Tracheobronchial Smooth Muscle

Grundström

Andersson

Wikberg

1981

Acta Pharmacologica et Toxicologica

109

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From the trachea, main bronchi and hilus bronchi of guinea pigs, preparations were isolated for registration of mechanical tension on electrical field stimulation and drugs. The trachea contracted on short trains of electrical stimulation. Usually these contractions were followed by a relaxant response. The contractions were abolished while the relaxations were potentiated by atropine. In the main bronchi field stimulation induced a contractile response which was abolished by atropine. In the latter preparation relaxant responses were never observed, even not after atropine. In the trachea and main bronchi neither the α1 blocker prazosin nor the α2 blocker yohimbine affected the contractile or relaxant responses when used in α‐blocking concentrations. The relaxant response in trachea was neither affected by the “P1 blocker” theophylline nor by the “P2 blocker” quinidine but it was partially inhibited by guanethidine or β‐blocking agents. The hilus bronchi contracted on field stimulation as well as by histamine. The contractile response on electrical stimulation was only slightly inhibited by atropine or guanethidine. In all three preparations responses on field stimulation were abolished by tetrodotoxin. It is suggested that in both trachea and main bronchi excitatory cholinergic innervation is present. Further, the trachea but not the main bronchi is innervated by both adrenergic and nonadrenergic inhibitory nerves. The hilus bronchi contains a non‐adrenergic non‐cholinergic excitatory nervous system.

show abstract

Pharmacological differences of non-adrenergic inhibitory response and of ATP-induced relaxation in guinea-pig tracheal strip-chains

Cited by 35 publications

References 6 publications

Purinergic Signaling in the Airways

Purinergic Signaling in the Airways

Non‐adrenergic, non‐cholinergic neural activation stabilizes smooth‐muscle tone independently of eicosanoid factors in guinea‐pig isolated airways

Pharmacological Characterization of the Autonomous Innervation of the Guinea Pig Tracheobronchial Smooth Muscle

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