Pharmacological characterization of three chicken melanocortin-3 receptor mutants

Zhang, H.-J.; Cui, Zhu; Liu, M.; Min, Tianqi; Xiao, Xia; Wang, Z.-Q.; Tao, Ya-Xiong

doi:10.1016/j.domaniend.2020.106507

Cited by 3 publications

(1 citation statement)

References 55 publications

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“…In addition, our current results are consistent with mammalian MC3Rs in that MC3R has little or no basal cAMP signaling [ 62 , 63 , 64 , 65 ]. Of interest, high constitutive activities were present in several non-mammalian MC3Rs, including teleosts [ 49 , 52 , 66 ], amphibians (Mexican axolotl) [ 67 ], and avian (chicken) [ 38 , 68 ]. The amino acids accounting for the differences in the constitutive activity between these MC3Rs are not clear.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Canine Melanocortin-3 and -4 Receptors by Melanocortin-2 Receptor Accessory Protein 1 and 2

Jiang

Tao

2022

Biomolecules

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The neural melanocortin receptors (MCRs), melanocortin-3 and -4 receptors (MC3R and MC4R), have crucial roles in regulating energy homeostasis. The melanocortin-2 receptor accessory proteins (MRAPs, MRAP1 and MRAP2) have been shown to regulate neural MCRs in a species-specific manner. The potential effects of MRAP1 and MRAP2 on canine neural MCRs have not been investigated before. Herein, we cloned canine (c) MC3R and identified one canine MRAP2 splice variant, MRAP2b, with N-terminal extension of cMRAP2a. Canine MC3R showed higher maximal responses to five agonists than those of human MC3R. We further investigated the modulation of cMRAP1, cMRAP2a, and cMRAP2b, on cMC3R and cMC4R pharmacology. For the cMC3R, all MRAPs had no effect on trafficking; cMRAP1 significantly decreased Bmax whereas cMRAP2a and cMRAP2b significantly increased Bmax. Both MRAP1 and MRAP2a decreased Rmaxs in response to α-MSH and ACTH; MRAP2b only decreased α-MSH-stimulated cAMP generation. For the MC4R, MRAP1 and MRAP2a increased cell surface expression, and MRAP1 and MRAP2a increased Bmaxs. All MRAPs had increased affinities to α-MSH and ACTH. MRAP2a increased ACTH-induced cAMP levels, whereas MRAP2b decreased α-MSH- and ACTH-stimulated cAMP production. These findings may lead to a better understanding of the regulation of neural MCRs by MRAP1 and MRAP2s.

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Section: Discussionmentioning

confidence: 99%

Modulation of Canine Melanocortin-3 and -4 Receptors by Melanocortin-2 Receptor Accessory Protein 1 and 2

Jiang

Tao

2022

Biomolecules

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Molecular cloning and functional characterization of melanocortin-3 receptor in grass carp (Ctenopharyngodon idella)

et al. 2022

Fish Physiol Biochem

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The role of G protein‐coupled receptors and their ligands in animal domestication

Kleinau,

Chini,

Andersson

et al. 2024

Animal Genetics

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The domestication of plants and animals has resulted in one of the most significant cultural and socio‐economical transitions in human history. Domestication of animals, including human‐supervised reproduction, largely uncoupled particular animal species from their natural, evolutionary history driven by environmental and ecological factors. The primary motivations for domesticating animals were, and still are, producing food and materials (e.g. meat, eggs, honey or milk products, wool, leather products, jewelry and medication products) to support plowing in agriculture or in transportation (e.g. horse, cattle, camel and llama) and to facilitate human activities (for hunting, rescuing, therapeutic aid, guarding behavior and protecting or just as a companion). In recent years, decoded genetic information from more than 40 domesticated animal species have become available; these studies have identified genes and mutations associated with specific physiological and behavioral traits contributing to the complex genetic background of animal domestication. These breeding‐altered genomes provide insights into the regulation of different physiological areas, including information on links between e.g. endocrinology and behavior, with important pathophysiological implications (e.g. for obesity and cancer), extending the interest in domestication well beyond the field. Several genes that have undergone selection during domestication and breeding encode specific G protein‐coupled receptors, a class of membrane‐spanning receptors involved in the regulation of a number of overarching functions such as reproduction, development, body homeostasis, metabolism, stress responses, cognition, learning and memory. Here we summarize the available literature on variations in G protein‐coupled receptors and their ligands and how these have contributed to animal domestication.

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Pharmacological characterization of three chicken melanocortin-3 receptor mutants

Cited by 3 publications

References 55 publications

Modulation of Canine Melanocortin-3 and -4 Receptors by Melanocortin-2 Receptor Accessory Protein 1 and 2

Modulation of Canine Melanocortin-3 and -4 Receptors by Melanocortin-2 Receptor Accessory Protein 1 and 2

Molecular cloning and functional characterization of melanocortin-3 receptor in grass carp (Ctenopharyngodon idella)

The role of G protein‐coupled receptors and their ligands in animal domestication

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