Pharmacological analysis of ovarial patency in Heliothis virescens

Pszczółkowski, Maciej A.; Peterson, Ashley; Srinivasan, Asoka; Ramaswamy, S.

doi:10.1016/j.jinsphys.2005.01.008

Cited by 34 publications

(30 citation statements)

References 22 publications

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“…In addition, the positive response of BgNrv1 mRNA to JH suggests a connexion between the enzyme and this hormone. This possible regulation of an Na + , K + -ATPase by JH was first proposed some years ago for Rhodnius prolixus (Ilenchuk and Davey, 1987), Locusta migratoria (Sevala and Davey, 1989) and Heliothis virescens (Pszczolkowski et al, 2005) in relation to the patency regulation in follicular cells, thus suggesting that the reduction in cell volume could occur as a result of ion exchange between the cell and the extracellular media. In the ovary of D. melanogaster, where vitellogenesis is controlled by 20-hydroxyecdysone, this hormone modifies ATPase activation (Bohrmann, 1991).…”

Section: Discussionmentioning

confidence: 81%

RNAi reveals the key role of Nervana 1 in cockroach oogenesis and embryo development

Irles¹,

Silva-Torres²,

Piulachs³

2013

Insect Biochemistry and Molecular Biology

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Na+ , K + -ATPases is a heterodimer protein consisting of α-and β-subunits that control the ion transport through cell membranes . In insects the β-subunit of the Na + , K + -ATPase, known as Nervana, was characterized as a nervous system-specific glycoprotein antigen from adult Drosophila melanogaster heads. Nervana is expressed ubiquitously in all insect tissues, and in epithelial cells appeared located in a basolateral position as part of the septate junctions. Herein we study two Nervana isoforms fromBlattella germanica, a cockroach species with panoistic ovaries. The sequencing and the phylogenetic analysis results suggest that these two isoforms are orthologs of D.melanogaster Nervana 1 and Nervana 2, respectively. Nervana 1 is highly expressed in the ovary of B. germanica, and depleting its expression results in changes in oocyte shape that do not impair oviposition. However, the resulting embryos show different defects and never hatch. These findings highlight the importance of this type of membrane pump in insect oogenesis as well as in embryo development, and its possible regulation by juvenile hormone.

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Section: Discussionmentioning

confidence: 81%

RNAi reveals the key role of Nervana 1 in cockroach oogenesis and embryo development

Irles¹,

Silva-Torres²,

Piulachs³

2013

Insect Biochemistry and Molecular Biology

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“…In vitro studies have suggested that JH binds to an unknown membrane protein on R. prolixus follicle cells (Ilenchuk and Davey, 1985) and activates PKC, which then phosphorylates the alpha subunit of Na + /K + ATPase, leading to the decrease in cell volume (Davey, 1996). In Heliothis virescens, JH-I regulates the patency development primarily via a G-protein coupled receptor (GPCR) and a cyclic adenosine monophosphate (cAMP)-dependent pathway (Pszczolkowski et al, 2005), while JH-II and JH-III presumably evoke patency by stimulation of the PLC-PKC pathway (Pszczolkowski et al, 2008). In Ae.…”

Section: Discussionmentioning

confidence: 99%

Protein kinase C modulates transcriptional activation by the juvenile hormone receptor methoprene-tolerant

Ojani

Liu

et al. 2016

Insect Biochemistry and Molecular Biology

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Juvenile hormone (JH) controls many biological events in insects by triggering dramatic changes in gene expression in target cells. The Methoprene-tolerant (MET) protein, an intracellular JH receptor, acts as a transcriptional regulator and binds to the promoters of tissue- and stage-specific JH target genes when JH is present. Our recent study has demonstrated that the transcriptional activation by MET is modulated by a membrane- initiated JH signaling pathway, involving phospholipase C (PLC) and calcium/calmodulin-dependent protein kinase II (CaMKII). Here we report that protein kinase C (PKC) is another essential intermediate of this pathway. PKC was activated by JH and this action was PLC-dependent. Inhibition of the PKC activity substantially weakened the JH-induced gene expression in mosquito cells. RNAi experiments indicated that several PKC isoforms were involved in the JH action during the post- emergence development of adult female mosquitoes. JH treatment considerably increased the binding of MET to the promoters of JH response genes in cultured mosquito abdomens that were collected from newly emerged female adults. The JH-induced DNA binding of MET was hindered when the abdomens were treated with a PKC inhibitor and JH. Therefore, the results suggest that PKC modulates the transactivation activity of MET by enhancing the binding of MET to JH response elements in the JH target genes. This mechanism may allow for variable and stage- and tissue-specific genomic responses to JH.

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“…In H. virescens , JH II and JH III-regulated patency is associated with voltage-dependent Ca 2+ channels, while JH I regulated patency appears to be Ca 2+ independent18. Pszczolkowski et al 11. found that JH I and JH III evoked patency in H. virescens through different second messenger pathways, cAMP-dependent and inositol triphosphate/diacylglycerol signal pathway, respectively.…”

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confidence: 99%

Identification of G protein-coupled receptors required for vitellogenin uptake into the oocytes of the red flour beetle, Tribolium castaneum

Bai

Palli

2016

Sci Rep

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Previous studies suggested that a membrane receptor might be involved in mediating vitellogenin (Vg) uptake and juvenile hormone (JH)-regulated remodeling of follicular epithelium (also called ‘patency’). G protein-coupled receptor (GPCR) family is one of the largest membrane receptor protein families and controls many key physiological processes. To investigate the role of GPCRs in insect reproduction and juvenile hormone-regulated Vg uptake, we performed a comprehensive RNA interference (RNAi) screen targeting GPCRs in the red flour beetle, Tribolium castaneum. Out of 112 GPCRs tested, knockdown of 41 GPCRs resulted in a reduction in fecundity. Interestingly, RNAi against two GPCRs (a Rhodopsin-like receptor and a Dopamine D2-like receptor) led to a significant reduction in Vg accumulation in developing oocytes. Functional assays of these two GPCRs showed that JH triggers a dose-dependent inhibition of intracellular cAMP levels in HEK293 cells expressing Tribolium Dopamine D2-like receptor. These data suggest that Dopamine D2-like receptor plays crucial roles in regulating Vg uptake and is a promising candidate membrane receptor mediating JH regulation of patency in the red flour beetle.

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Pharmacological analysis of ovarial patency in Heliothis virescens

Cited by 34 publications

References 22 publications

RNAi reveals the key role of Nervana 1 in cockroach oogenesis and embryo development

RNAi reveals the key role of Nervana 1 in cockroach oogenesis and embryo development

Protein kinase C modulates transcriptional activation by the juvenile hormone receptor methoprene-tolerant

Identification of G protein-coupled receptors required for vitellogenin uptake into the oocytes of the red flour beetle, Tribolium castaneum

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