Pharmacological Agents and Erythroid Colony Formation: Effects of Beta-Adrenergic Agonists and Steroids

Fisher, James W.; Ohno, Y; Mödder, B; Przala, F; Fink, Gregory D.; Gross, Dennis M.

doi:10.1007/978-1-4612-6301-2_18

Cited by 3 publications

(4 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These in vivo findings in rabbits contrast with earlier studies carried out by a cooperative group study utilizing polycythemic mice as a model test system (30). In any event, it appears from recent work that 5/~-DHT is clearly more effective in stimulating erythroid differentiation in vivo in the rabbit than is 5a-DHT, and that this relative potency of 5/~-DHT over 5a-DHT extends to cultured erythroid cells from this species (28). The site of erythropoietin (Ep) action has been considered to appear at the developmental stage between BFU-E and CFU-E.…”

Section: Discussioncontrasting

confidence: 70%

“…It should be noted that on a molar basis steroid metabolites are at least 100-fold more active in stimulating erythroid colony formation from human bone marrow cells than they are in inducing ALA-S or porphyrin formation in avian liver cells. This sensitivity of bone marrow cells to steroids including glucocorticoids extends to other species such as rats (15) rabbits (28), and man (29), suggesting an intrinsic biological characteristic of these cell types as compared with hepatic cells. Stimulation of BFU-E formation by the coprostane compounds is of particular interest because these neutral sterol metabolites of cholesterol are produced in large amounts in man.…”

Section: Discussionmentioning

confidence: 75%

“…The structure-inducibility relationship of these steroid metabolites to BFU-E, the most primitive erythroid progenitor cells, has not been extensively examined, except for the single pair of metabolites studied by Fisher et al (28). These authors found, utilizing rabbit bone marrow cultures, that both the parent hormone, testosterone, and its metabolites, 5a-and 5/~-dihydrotesterone (DHT) stimulated the in vitro growth of BFU-E in the presence of erythropoietin but that 5~-DHT was significantly more potent than the 5a derivative of the hormone (5a-DHT) in this action (28). When animals were pretreated with these steroids, both testosterone and 5/~-DHT, but not 5a-DHT, gave rise to increased erythroid-colony formation in culture (28).…”

Section: Discussionmentioning

confidence: 99%

“…These authors found, utilizing rabbit bone marrow cultures, that both the parent hormone, testosterone, and its metabolites, 5a-and 5/~-dihydrotesterone (DHT) stimulated the in vitro growth of BFU-E in the presence of erythropoietin but that 5~-DHT was significantly more potent than the 5a derivative of the hormone (5a-DHT) in this action (28). When animals were pretreated with these steroids, both testosterone and 5/~-DHT, but not 5a-DHT, gave rise to increased erythroid-colony formation in culture (28). These in vivo findings in rabbits contrast with earlier studies carried out by a cooperative group study utilizing polycythemic mice as a model test system (30).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

The influence of steroid hormone metabolites on the in vitro development of erythroid colonies derived from human bone marrow.

Urabe¹,

Sassa²,

Kappas³

1979

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Earlier studies from this and other laboratories demonstrated that certain naturally occurring steroid metabolites, formerly considered to be physiologically inactive end products of hormone metabolism, strongly stimulate porphyrin and &aminolevulinic acid synthase (ALA-S) 1 formation in cultured chick embryo liver eeUs (1, 2) as well as in vivo in the liver of the chick embryo (3). Steroid metabolites with a 5fl-structure (A:B cis) were more potent in this respect than their 5a-epimers or their parent compounds. 5fl-steroids which induce porphyrin synthesis in chick embryo liver cells have also been shown to induce the early synthesis of hemoglobin in the erythroblasts of the chick blastoderm (4) and a 5fl-steroid receptor in this primitive avian tissue has been identified (5).These steroid metabolites have been also reported to stimulate erythropoiesis in vivo in mice. For example, Gorshein and Gardner (6) described enhanced erythropoiesis in exhypoxic polycythemic mice after 5fl-steroid administration as determined by 59Fe incorporation into peripheral erythrocytes. Gordon et al. (7) reported similar results using both normal and poiycythemic mice; in addition, their study showed that an antibody preparation against erythropoietin abolished the effects of testosterone, but not those of 5fl-steroids. These data thus suggested that 5fl-steroids can have effects on erythropoiesis that are independent of the action of erythropoietin. Besa et al. (8) later demonstrated that the 5fl-compound, etiocholanolone (5fl-androstan-3a-ol-17-one), increased erythrocyte mass in squirrel monkeys, confirming that such steroid metabolites may stimulate erythropoiesis not only in the erythroid tissues of birds, rodents, but also in vivo in primates. 5fl-steroids have also been demonstrated to increase both heme and globin synthesis in suspension cultures of human bone marrow cells (9, 10).The development of newer methodology for the study of hematopoietic stem cells in culture (11-13) made it possible to examine the mechanism and site of action of erythropoietin and steroids on the development of erythroid colonies in semisolid media (14). Using rat bone marrow cells, Singer et al. (15) reported that certain steroids enhanced erythroid colony growth in vitro and suggested that etiocholanolone, a prototype 5fl-steroid, may act on early erythroid progenitor cells (16).

show abstract

Section: Discussioncontrasting

confidence: 70%

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The influence of steroid hormone metabolites on the in vitro development of erythroid colonies derived from human bone marrow.

Urabe¹,

Sassa²,

Kappas³

1979

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

Mechanism of extramedullary haematopoiesis in rabbits with saponin-induced myelofibrosis and myeloid metaplasia

Wang¹,

Tobin²

1982

Br J Haematol

View full text Add to dashboard Cite

The saponin-induced myelofibrosis and myeloid metaplasia model in the rabbit was used to study mechanisms of extramedullary haematopoiesis. Haematopoietic progenitor cells, erythroid colony forming units (CFU-E) and burst forming units (BFU-E) were assayed serially in the peripheral blood, spleen and bone marrow after saponin administration, employing the in vitro methylcellulose culture technique. Animals that had undergone splenectomy prior to saponin administration were also studied. The results demonstrated increases of progenitor cells in the blood and spleen and a simultaneous depletion of such cells in marrow after saponin treatment. The results in splenectomized animals were similar to those observed in non-splenectomized animals after saponin administration. The findings indicate that following saponin administration there is a release of CFU-E and BFU-E from bone marrow into periphery and probably deposition in the spleen, and suggest that myeloid metaplasia in myelofibrosis may result from colonization of extramedullary sites originating from the bone marrow.

show abstract

Mechanism of extramedullary haematopoiesis in rabbits with saponin-induced myelofibrosis and myeloid metaplasia

Wang¹,

Tobin²

1982

Br J Haematol

View full text Add to dashboard Cite

Pharmacological Agents and Erythroid Colony Formation: Effects of Beta-Adrenergic Agonists and Steroids

Cited by 3 publications

References 45 publications

The influence of steroid hormone metabolites on the in vitro development of erythroid colonies derived from human bone marrow.

The influence of steroid hormone metabolites on the in vitro development of erythroid colonies derived from human bone marrow.

Mechanism of extramedullary haematopoiesis in rabbits with saponin-induced myelofibrosis and myeloid metaplasia

Mechanism of extramedullary haematopoiesis in rabbits with saponin-induced myelofibrosis and myeloid metaplasia

Contact Info

Product

Resources

About