Pharmacokinetics study of 16 active ingredients from Tabson‐2 decoction in normal and d‐galactose induced osteoporosis rats by liquid chromatography–tandem mass spectrometry

Abstract

Tabson‐2 decoction is the traditional Mongolian formula for anti‐osteoporosis, and the ambiguous of active ingredient is an important factor in restricting its modernization and globalization. Although pharmacokinetic profiles research is a viable approach to find the components being responsible for formula efficacy, the pharmacokinetics study of Tabson‐2 decoction has not been elucidated yet. Owing to the existence of isomers, low bioavailability of some small molecule and interference of endogenous, the pharmacokinetics study of Tabson‐2 decoction are more difficult than that of chemical drugs. In our experiment, a specific and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous determination of 16 active ingredients in Tabson‐2 decoction, which could fulfill the requirements of multi‐compounds pharmacokinetic study of Tabson‐2 decoction. Additionally, the ingredients with significant distributions in rats were gentianic acid, chlorogenic acid, and aucubin, which could be the main potential active components in Tabson‐2 decoction. The components with a significant bioavailability difference between normal and d‐galactose induced osteoporosis rats were achieved as well. These data offer useful information for screening the active ingredients in Tabson‐2 decoction, and assessing the bioavailability of these active ingredients in different physiological status, which might provide a possible mechanism of anti‐osteoporosis efficacy of Tabson‐2 decoction.

HPLC‐MS/MS based comparative pharmacokinetics of 12 bioactive components in normal and osteoporosis rats after oral administration of You‐Gui‐Wan

“…The safety and efficacy of therapeutics are dependent upon the movement of drugs in patients (pharmacokinetics) and the pharmacological response of the patients to specific agents (pharmacodynamics) [26,31]. The pharmacokinetic parameters in pathological conditions are often altered, which are distinctly different from normal physiological parameters [23][24][25][26]. Moreover, traditional Chinese medicines are prescribed for disease conditions during clinical treatment.…”

“…Nevertheless, many traditional Chinese medicines have been evaluated in healthy animals or human subjects [21,22]. Considering that pathological conditions are an essential factor affecting the efficacy of drugs [23][24][25][26], it is necessary to investigate the potential pharmacokinetic alterations of You-Gui-Wan in patients with osteoporosis.…”

Applying Four-Steps Characteristic Ion Filtering with HPLC-QE Orbitrap MS Approach to Rapid Characterize Compound Structure s and Determine Major Representative Components in Modified Tabusen-2 Decoction

“…Herein, in order to shorten the analysis time, improve the analysis sensitivity, and simultaneously determine UV-absorbing compounds and Non-UV-absorbing compounds, high performance liquid chromatography coupled with mass spectrometry (HPLC-QE Orbitrap MS) approach [31], as a high e ciency, FSCIF and sensitive technology, is employed in this study to simultaneouses isolate and identify the material basis in MTBD. Additionally, the existence of isomer (ICGAA with 1, 5-DQA, GE with APG, LT with KPF) in MTBD increases the di culty of separation and analysis [32]. The chromatographic conditions in quantitative analysis need to be optimized carefully during present research.…”

Applying Four-Step Characteristic Ion Filtering with HPLC-Q-Exactive MS/MS Spectrometer Approach for Rapid Compound Structures Characterization and Major Representative Components Quantification in Modified Tabusen-2 Decoction

“…Herein, in order to shorten the analysis time, improve the analysis sensitivity, and simultaneously determine UV-absorbing compounds and non-UV-absorbing compounds, high performance liquid chromatography coupled with mass spectrometry (HPLC-Q-Exactive MS/MS spectrometer) approach [34], as a high efficiency, is employed in this study to separate and identify the material basis in MTBD. Additionally, the existence of isomer (ICGAA with 1,5-DQA, GE with APG, LT with KPF) in MTBD increases the difficulty of separation and analysis [35].…”