Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets

Liu, Yu; Zhao, Wenhua; Xu, Jiaming; Yu, Xiaolin; Yuan, Chun; Fu, Shulin; Qiu, Yinsheng

doi:10.1111/jvp.12797

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…Baicalin was purchased from National Institutes for Food and Drug Control (B110715-201318, Beijing, China). Sodium baicalin was prepared at the Hubei Key Laboratory of Animal Nutrition and Feed Science (Wuhan, China) and was >95% pure [52]. Ethyl pyruvate (EP) and flunixin meglumine (FM) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) and Guangdong WenS Dahuanong Biotechnology Co., Ltd. (Yunfu, China), respectively.…”

Section: Experimental Productsmentioning

confidence: 99%

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Zhang

et al. 2021

Molecules

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Glaesserella parasuis (G. parasuis) causes inflammation and damage to piglets. Whether polyserositis caused by G. parasuis is due to tight junctions damage and the protective effect of baicalin on it have not been examined. Therefore, this study aims to investigate the effects of baicalin on peritoneal tight junctions of piglets challenged with G. parasuis and its underlying molecular mechanisms. Piglets were challenged with G. parasuis and treated with or without baicalin. RT-PCR was performed to examine the expression of peritoneal tight junctions genes. Immunofluorescence was carried out to detect the distribution patterns of tight junctions proteins. Western blot assays were carried out to determine the involved signaling pathways. Our data showed that G. parasuis infection can down-regulate the tight junctions expression and disrupt the distribution of tight junctions proteins. Baicalin can alleviate the down-regulation of tight junctions mRNA in peritoneum, prevent the abnormalities and maintain the continuous organization of tight junctions. Our results provide novel evidence to support that baicalin has the capacity to protect peritoneal tight junctions from G. parasuis-induced inflammation. The protective mechanisms of baicalin could be associated with inhibition of the activation of PKC and MLCK/MLC signaling pathway. Taken together, these data demonstrated that baicalin is a promising natural agent for the prevention and treatment of G. parasuis infection.

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Section: Experimental Productsmentioning

confidence: 99%

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Zhang

et al. 2021

Molecules

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“…Baicalin was purchased from National Institutes for Food and Drug Control (B110715-201318, Beijing, China). Sodium baicalin (>95% pure) was synthetized at Hubei key Laboratory of Animal Nutrition and Feed Science (Wuhan, China) (32). Flunixin meglumine (FM) and ethyl pyruvate (EP), used as positive controls, were purchased from Guangdong WenS Dahuanong Biotechnology Co. Ltd. (Yunfu, China) and Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China), respectively.…”

Section: Drugsmentioning

confidence: 99%

“…After oral administration, baicalin is poorly absorbed in the intestinal tract for its high polarity and displays enterohepatic recycling and a complex metabolism (31). Our previous research showed that baicalin is highly absorbed and utilized using its sodium salt after intramuscular administration and suitable for treating infectious diseases in piglets (32).…”

Section: Introductionmentioning

confidence: 99%

Baicalin Protects Vascular Tight Junctions in Piglets During Glaesserella parasuis Infection

Liu

Zhang

et al. 2021

Front. Vet. Sci.

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Glaesserella parasuis (G. parasuis) can cause Glässer's disease and severely affect swine industry worldwide. This study is an attempt to address the issue of the capability of G. parasuis to damage the vascular barrier and the effects of baicalin on vascular tight junctions (TJ) in order to investigate the interactions between the pathogen and the porcine vascular endothelium. Piglets were challenged with G. parasuis and treated with or without baicalin. The expressions of vascular TJ genes were examined using RT-PCR. The distribution patterns of TJ proteins were detected by immunofluorescence. The involved signaling pathways were determined by Western blot assays on related proteins. G. parasuis can downregulate TJ expression and disrupt the distribution of TJ proteins. Baicalin can alleviate the downregulation of vascular TJ mRNA, maintain the distribution, and prevent the abnormalities of TJ. These results provide ample evidence that baicalin has the capacity to protect vascular TJ damaged by G. parasuis through inhibiting PKC and MLCK/MLC pathway activation. As a result, baicalin is a promising candidate for application as a natural agent for the prevention and control of G. parasuis infection.

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“…Baicalin acts poor solubility (160 µg/mL) and low oral bioavailability (2.2% after oral administration to rats). Our previous study on the pharmacokinetics of sodium baicalin found that an intramuscular injection of sodium baicalin in piglets resulted in rapid and complete absorption, with a mean maximal plasma concentration of 77.28 ± 7.40 µg/mL at 0.17 hr and a high absolute bioavailability of 83.73 ± 5.53% [20]. Meanwhile, our previous study also showed that baicalin can inhibit the apoptosis and inflammation of porcine peripheral monocytes and porcine vascular endothelial cells induced by baicalin, and the inhibitory mechanism may be related to the inhibition of the PKC-MAPK signaling pathway [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Baicalin Alleviate Apoptosis via PKC-MAPK Pathway in Porcine Peritoneal Mesothelial Cells Induced by Glaesserella parasuis

Zhou

Wang

et al. 2022

Molecules

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Glaesserella parasuis (GPS), a causative agent of Glässer’s disease, is thought to be the main fatal cause of peritonitis in swine, thus resulting in high mortality and morbidity and significant economic losses to the swine industry. However, the mechanisms of GPS infection-induced apoptosis and possible therapeutic pathway for GPS infection in peritonitis remain unclear. Baicalin has important biological functions during disease treatment, such as antiviral, bacterial inhibition, anti-apoptosis, and anti-inflammatory. However, whether baicalin has anti-apoptotic effects during the process of GPS infection in peritonitis is unclear. In the present study, the anti-apoptotic effect and mechanisms of baicalin in GPS infection-induced apoptosis were investigated in porcine peritoneal mesothelial cells (PPMC). The results showed that baicalin could inhibit the apoptosis rate occurrence of PPMC induced by GPS to various degrees and inhibit the expression of apoptosis-related genes and cleaved caspase-3. Meanwhile, baicalin significantly antagonized the expression of p-JNK, p-p38, and p-ERK induced by GPS in PPMC. These findings for the first time demonstrate that baicalin exerted the effect of antagonizing GPS induced apoptosis in PPMC by inhibiting the activation of the PKC-MAPK pathway and could be a therapeutic option in the management of GPS infection.

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Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets

Cited by 5 publications

References 20 publications

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Baicalin Protects Vascular Tight Junctions in Piglets During Glaesserella parasuis Infection

Baicalin Alleviate Apoptosis via PKC-MAPK Pathway in Porcine Peritoneal Mesothelial Cells Induced by Glaesserella parasuis

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