Pharmacokinetics of Salicylic Acid in an Interaction Study of Aqueous Extract of Hibiscus Sabdariffa L With Aspirin in Rat Plasma

Abstract

This study intends to find out whether co-administration of aqueous extract of Hibiscus sabdariffa L and aspirin would affect the pharmacokinetics profile of aspirin's metabolite, namely salicylic acid. Four groups of Sprague Dawley rats (n=6/group) received aspirin alone (10 mg/kg orally), aqueous extract of Hibiscus sabdariffa L 62.5 mg/kg with Aspirin 10 mg/kg, orally, aqueous extract of Hibiscus sabdariffa L 125 mg/kg with Aspirin 10 mg/kg, orally, and aqueous extract of Hibiscus sabdariffa L 250 mg/kg with Aspirin 10 mg/kg, orally. Aqueous extract of Hibiscus sabdariffa L was given once daily for seven days. On the 7th day, 30 minutes after administration of aqueous extract of Hibiscus sabdariffa L followed by administration of aspirin, then the plasma sample was taken at a different point. HPLC-UV method was used to identify the pharmacokinetics profile of salicylic acid contained in rat plasma. Co-administration of aqueous extract of Hibiscus sabdariffa L and aspirin showed an increase in AUC, Cmax, and t1/2 of salicylic acid, but the increase was not significant (p>0.05). In conclusion, aqueous extract of Hibiscus sabdariffa L does not affect the pharmacokinetics profile of salicylic acid