Pharmacokinetics of 10-Hydroxy Mesaconitine in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry

Yang, Jinzhao; Zeng, Guowu; Ma, Jianshe; Wang, Xianqin; Zhou, Quan

doi:10.1155/2021/6640184

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…LC-MS/MS is characterized by a high detection sensitivity, and the samples can display a good response signal in LC-MS/MS, even at low concentrations [ 18 – 20 ]. The specified precursor ion and product ion transition pair can be monitored, resulting in high specificity [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Determination of Senegenin and Tenuifolin in Mouse Blood by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and Their Pharmacokinetics

Shen

Dai

et al. 2022

International Journal of Analytical Chemistry

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An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of senegenin and tenuifolin in mouse blood was developed. The pharmacokinetics of senegenin and tenuifolin in mice after intravenous (5 mg/kg) and oral (60 mg/kg) administration were studied, and the absolute bioavailability was calculated. A CORTECS T3 column was used, with a column temperature set at 40°C. The mobile phase was acetonitrile and 0.1% formic acid. Gradient elution was adopted, using a flow rate of 0.4 mL/min and an elution time of 4 min. Quantitative analysis was performed using electrospray ionization (ESI) with multiple reaction monitoring (MRM) in negative ion mode. Institute of Cancer Research (ICR) mice were bled from the tail vein after intravenous or oral administration of senegenin and tenuifolin. A UPLC-MS/MS method was established to determine the blood concentrations of each drug in mice, and the noncompartmental model was used to fit the pharmacokinetic parameters. Senegenin and tenuifolin showed a good linear relationship (r > 0.995) within a concentration range of 5–400 ng/mL in mouse blood. The intraday precision was <12%, the interday precision was <14%, and the accuracy was 87–109%. The recovery was >88%, and the matrix effect was 87–94%. The oral bioavailability of senegenin and tenuifolin in mice was 8.7% and 4.0%, respectively. The established UPLC-MS/MS method is suitable for pharmacokinetic studies of senegenin and tenuifolin in mice.

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Section: Discussionmentioning

confidence: 99%

Determination of Senegenin and Tenuifolin in Mouse Blood by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and Their Pharmacokinetics

Shen

Dai

et al. 2022

International Journal of Analytical Chemistry

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“…The residue was reconstituted in 50 μ L acetonitrile-water (5 : 95 v/v), vortex-mixed, and centrifuged again under the above-mentioned conditions [ 38 ]. 2 μ L of this solution was injected into the UPLC-MS/MS for analysis [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

“…200 μL of the combined working solution was added to 50 μL plasma and 20 μL IS (1 μg•mL −1 ) to obtain the calibration standards at 62, e residue was reconstituted in 50 μL acetonitrile-water (5 : 95 v/v), vortex-mixed, and centrifuged again under the above-mentioned conditions [38]. 2 μL of this solution was injected into the UPLC-MS/MS for analysis [39].…”

Section: Preparation Of Stock and Standard Solutionsmentioning

confidence: 99%

Pharmacokinetic Study of Coadministration with Cefuroxime Sodium for Injection Influencing ReDuNing Injection-Derived Seven Phytochemicals and Nine Metabolites in Rats

Zhao¹,

Wang²,

Cheng³

et al. 2022

Journal of Analytical Methods in Chemistry

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According to the sixth edition of China’s “New Coronavirus Diagnosis and Treatment Plan (NCDTP),” ReDuNing injection (RDN) was firstly introduced to treat severe and critical COVID-19, whereas its combination with broad-spectrum antibiotics was suggested to take with extreme caution and full reasons. Therefore, we aim to describe the pharmacokinetics of seven active phytochemicals and semiquantification of nine relevant metabolites in ReDuNing injection (RDN) after combining with cefuroxime sodium (CNa) for injection in rat plasma. Male Sprague–Dawley rats were randomly assigned to six groups, and they were intravenously administered, respectively, with different prescriptions of RDN (2 mL/kg) and CNa (225 mg/kg). At different time points (0.03, 0.08, 0.17, 0.24, 0.33, 0.50, 0.67, 1, and 6 h) after administration, the drug concentrations of iridoids glycosides, organic acids, and metabolites in rat plasma were determined using ultrahigh-pressure liquid chromatography coupled with linear ion rap-orbitrap tandem mass spectrometry (UHPLC–LTQ–Orbitrap–MS), and main pharmacokinetic parameters were estimated by noncompartment model. The results showed that there were differences in pharmacokinetic parameters, AUC(0-t), T1/2, Cmax, CL of iridoids glycosides, and organic acids, after the intravenous administration of the different combinations of RDN and CNa. Moreover, different combinations of the injections also resulted in different curves of relative changes of each metabolite. The obtained results suggested that RDN and CNa existed pharmacokinetic drug–herb interactions in rats. The findings not only lay the foundation for evaluating the safety of RDN injection combined with CNa but also make contributions to clinically applying RDN injection combined with CNa, which works potentially against severe forms of COVID-19.

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“…There were several LC-MS or LC-MS/MS methods developed for determination of curdione in rat or rabbit plasma [19][20][21][22][23][24], however, no UPLC-MS/MS method developed for determination of curdione in mice and its pharmacokinetics. Chromatographic detection methods have long analysis time, poor selectivity for complex matrices, low sensitivity, and cannot achieve effective detection of the drugs; UPLC-MS/MS due to its fast analysis speed, it has the advantages of high sensitivity and strong specificity [25][26][27][28][29][30]. In this study, the protein precipitation method was used to extract curdione in blood samples, and an UPLC-MS/MS method was established for the determination of curdione in mouse blood with high sensitivity and high specificity, and this method was successfully applied to the in vivo pharmacokinetics of curdione after gavage and intravenous injection in mice.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and bioavailability of curdione in mice by UPLC-MS/MS

Liang

Chen

Luo

et al. 2023

AChrom

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A UPLC-MS/MS method was developed to determinate curdione in the mouse blood, and the pharmacokinetics of curdione in mice after intravenous (5 mg kg−1) and oral (20 mg kg−1) administration were studied. The HSS T3 column was used for separation, and column temperature was set at 40 °C. Multiple reaction monitoring (MRM) mode were used for determination of curdione. Blood samples were taken from the caudal vein of Institute of Cancer Research (ICR) mice after administration of curdione. It showed a good linear relationship in the range of 1–500 ng mL−1 (r > 0.998); the intra-day precision was <13%, the inter-day precision was <15%, and the accuracy was 90%–105%, the recovery was >77%, and the matrix effect was 97%–107%. The half-life was relatively short, and the bioavailability was 6.5%. The developed method was suitable for the pharmacokinetics of curdione in mice.

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Pharmacokinetics of 10-Hydroxy Mesaconitine in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry

Cited by 3 publications

References 24 publications

Determination of Senegenin and Tenuifolin in Mouse Blood by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and Their Pharmacokinetics

Determination of Senegenin and Tenuifolin in Mouse Blood by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and Their Pharmacokinetics

Pharmacokinetic Study of Coadministration with Cefuroxime Sodium for Injection Influencing ReDuNing Injection-Derived Seven Phytochemicals and Nine Metabolites in Rats

Pharmacokinetics and bioavailability of curdione in mice by UPLC-MS/MS

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