Pharmacokinetic Study on the Multi-constituents of Huangqin-Tang Decoction in Rats

Abstract

Using a validated high-performance liquid chromatographic (HPLC) method, the pharmacokinetics of multi-constituents in Huangqin-Tang decoction were simultaneously studied both in the compound prescription and in each single herb decoction. At different intervals (0, 1, 2, 4, 8, 12, 24, 36, 48 h) after oral administration of the Huangqin-Tang decoction or a single herb decoction at a dose of 10 g x kg(-1), the concentrations of the constituents and their metabolites: baicalin (BG), wogonoside (WG), oroxylin-A-glucuronide (OG), baicalein (B), wogonin (W), oroxylin-A (O), paeoniflorin (PF), paeonimetabolin-I (PM-I), liquiritin (LG), liquiritigenin (L), glycyrrhizic acid (GL) and glycyrrhetinic acid (GA), were detected in the rat plasma. A new metabolite-3,5,7,2',6'-penta hydroxy flavone (visidulin I, VD-I) was found in rat plasma after oral administration of Huangqin-Tang or a single herb Huangqin decoction, and the quality was identified by HPLC and LC/MS. The pharmacokinetic parameters of the constituents and metabolites in the compound prescription and single herb decoctions were compared. All concentration-time curves corresponded to the one-compartment model. The constituents of BG, WG, OG, VD-I and LG had higher C(max) and AUC(0-lim) in the compound prescription than in the single herb decoction, and WG had significant difference. The constituents of PF, W and O only had a higher AUC(0-lim) in the compound prescription, and O had a significant difference. It was concluded, in brief, that there were obvious differences in the pharmacokinetic parameters of most constituents (especially constituent WG) between the compound prescription and single herb decoction. The constituents in the compound prescription had delayed absorption and elimination, a longer residence time in the body, and higher C(max) and AUC(0-lim), than those in the single herb decoction. Therefore, they were more efficient and durable, making them promising to exerting pharmacological effects in vivo.

Influence of Supercritical CO₂ Extraction of Schisandra Chinensis on Antioxidant Status, Carcass and Meat Quality of AA Broilers

“…Schisandra lignans are the major constituents of Schisandra chinensis and more than 40 of them have been isolated, in which the most abundant ingredients include schisandrin, lignans, deoxyschizandrin etc. Several papers focused on how the individual component in the compound prescription interact with other ingredients (Zuo et al, 2003;Bochu et al, 2005;Di et al, 2006;Lu et al, 2007). Schisandra lignans have been shown to have various biological activities, they are usually considered as the main pharmacological effective compounds (Hancke et al, 1999), such as hepatoprotective effect, potent antioxidative property, anticarcinogenic activity through the activation of key liver enzymes and strong inhibitory effect on human immunodeficiency virus reported recently.…”

From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats

“…Most of these studies were focused on single licorice compounds such as glycyrrhizic acid (Makino et al, 2008), liquiritigenin (Shimamura et al, 1993), and isoliquiritigenin (Guo et al, 2008a,b;Cuendet et al, 2010). Some other studies attempted to detect the in vivo metabolites after licorice extracts were administered (Homma et al, 1997;Zuo et al, 2003;Hou et al, 2005). However, only a few compounds were detected in most reports.…”

Therapeutic Potential of Wogonin: A Naturally Occurring Flavonoid

“…Acute lethality of wogonin by oral administration to mice was low; its LD 50 was 3.9 g/kg (22). Following oral or intravenous administration of wogonin to rats its plasma levels can be measured by a modified HPLC (11,41,47). Tsai et al (41) studied plasma levels of wogonin following its intravenous administration and found that a rapid increase in its plasma level was followed by a prolonged elimination phase.…”