Pharmacokinetic study of paeoniflorin in mice after oral administration of Paeoniae radix extract

Chen, Lih Chi; Lee, Mei Hsien; Chou, Mei Huei; Lin, Muh-Shi; Yang, Lingling

doi:10.1016/s0378-4347(99)00408-9

Cited by 36 publications

(30 citation statements)

References 21 publications

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“…Furthermore, we have previously reported the pharmacokineics of paeoniflorin after oral administration of Paeoniae Radix extract (20). Compared with the results from oral administration of purified paeoniflorin by Takeda et al (18), our previous results indicated a later time to reach maximum concentration (tmax) and longer t1/2 after oral administration of Paeoniae Radix extracts (20).…”

mentioning

confidence: 51%

“…To calculate the administered dose, the content of paeoniflorin in SGT freeze-dried extract powder had been quantitatively analyzed according to the HPLC method we previously reported (20). The content of paeoniflorin in the freeze-dried powder of SGT was determined (9.31%) from the peak height ratios by using the equation for linear regression obtained from the calibration curve.…”

Section: Content Of Paeoniflorin In Sgt Freeze-dried Extract Powdermentioning

confidence: 99%

“…Preparation of plasma samples for analysis was described before (20). The same sample handling process was used for recovery and precision determination in plasma.…”

Section: Preparation Of Plasma Samples and Determination Of Paeoniflomentioning

confidence: 99%

“…The same sample handling process was used for recovery and precision determination in plasma. Plasma concentrations of paeoniflorin were assayed according to the HPLC method we previously reported (20).…”

Section: Preparation Of Plasma Samples and Determination Of Paeoniflomentioning

confidence: 99%

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Pharmacokinetics of Paeoniflorin After Oral Administration of Shao-yao Gan-chao Tang in Mice

Chen

Chou

Lin

et al. 2002

Japanese Journal of Pharmacology

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ABSTRACT-Paeoniflorin, a monoterpene glycoside, is the principal bioactive component of Paeoniae Radix. The traditional prescription Shao-yao Gan-chao Tang (SGT; Kampo: Shakuyaku-Kanzo-To), which is composed of Paeoniae Radix and Glycyrrhizae Radix, has been widely used in China and Japan. Quantification of paeoniflorin in mouse plasma after oral administration of SGT (at a dose containing 10 mg /kg paeoniflorin) was achieved using a simple and rapid high-performance liquid chromatography method. The plasma concentration-time curves were fitted with mean terminal half-lives (t 1 /2 ) of 116.17 min. The maximum plasma concentration (C max ) of paeoniflorin was 111.56 ng /ml, time to reach maximum concentration (t max ) was 17.00 min, the area under the plasma concentration-time curve (AUC) 0-t was 12293.42 ng · min /ml, clearance / bioavailability (CL /F) value was 644.74 ml /min · kg, apparent volume of distribution / bioavailability (V d /F) value was 103.05 l /kg, and the mean residence time (MRT) was 169.64 min. These results, together with the previously reported kinetic data of paeoniflorin after oral administration of Paeoniae Radix extract alone, indicated that absorption of paeoniflorin after oral administration of SGT was significantly greater than that after oral administration of Paeoniae Radix alone.

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mentioning

confidence: 51%

Section: Content Of Paeoniflorin In Sgt Freeze-dried Extract Powdermentioning

confidence: 99%

“…Preparation of plasma samples for analysis was described before (20). The same sample handling process was used for recovery and precision determination in plasma.…”

Section: Preparation Of Plasma Samples and Determination Of Paeoniflomentioning

confidence: 99%

Section: Preparation Of Plasma Samples and Determination Of Paeoniflomentioning

confidence: 99%

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Pharmacokinetics of Paeoniflorin After Oral Administration of Shao-yao Gan-chao Tang in Mice

Chen

Chou

Lin

et al. 2002

Japanese Journal of Pharmacology

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“…This is a cumbersome, complex process resulting in rather lower recoveries and involving the use of toxic solvents (Chen et al, 1999;Guan et al, 2003). In our method, 4.5% perchloric acid aqueous solution was used to denature the plasma protein (Keung et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Influence of co-administrated sinomenine on pharmacokinetic fate of paeoniflorin in unrestrained conscious rats

Liu

Zhou

Liu

et al. 2005

Journal of Ethnopharmacology

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Paeonia lactiflora Pall. (Ranunculaceae) root and Sinomenium acutum Rehder and Wilson (Menispermaceae) stem are two herbs widely used in Chinese medicine to treat rheumatoid arthritis. While, in theory, either herb could be used alone, in practice, Chinese medicine practitioners prescribe them together. Studies on pharmacokinetic interaction between the active constituents of these two herbs (paeoniflorin and sinomenine, respectively) provide empirical evidence to support their clinical practice. A single dose of paeoniflorin (150 mg/kg) alone and with sinomenine hydrochloride (90 mg/kg) was administered by gastric gavage to unrestrained conscious male Sprague-Dawley rats (n = 6, 250-300 g). Blood samples were collected periodically via a jugular vein before and after dosing from 10 min to 12 h. A high-performance liquid chromatographic (HPLC) assay was developed to determine the plasma concentrations of paeoniflorin. Non-compartmental pharmacokinetic profiles were constructed by using the software PK Solutions 2.0. The pharmacokinetic parameters were compared using unpaired Student t-test. After co-administration of sinomenine, the peak plasma concentration of paeoniflorin was elevated (P < 0.01), the peak time was delayed (P < 0.01), the AUC 0-t was increased (P < 0.001), the mean residence time (MRT) was prolonged (P < 0.01), the C L was decreased (P < 0.01) and the V d was reduced (P < 0.05). These results indicate that sinomenine hydrochloride at 90 mg/kg significantly improved the bioavailability of paeoniflorin in rats.

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Simultaneous determination of albiﬂorin and paeoniﬂorin in rat urine by solid‐phase extraction and high‐performance liquid chromatography following oral administration of Si–Wu decoction

Sheng

Zhang

et al. 2004

Biomedical Chromatography

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A high performance liquid chromatographic method (HPLC), together with solid phase extraction (SPE), was developed for simultaneous determination of albiflorin and paeoniflorin in rat urine after oral administration of Si-Wu decoction. The samples were pretreated with solid phase extraction using Extract-Cleantrade mark cartridges. Analysis of the extract was performed on a reversed-phase C18 column and a mobile phase made up of acetonitrile and 0.03% formic acid (17:83, v/v). UV detection was set at 230 nm. The assay was linear over the range 2.625-52.50 mg/mL for albiflorin and 3.875-77.50 microg/mL for paeoniflorin. The average percentage recoveries of three spiked urines were 97.01 +/- 3.32 and 102.32 +/- 6.97 for albiflorin and paeoniflorin, respectively. The intra-day precision (RSD) ranged from 0.21 to 1.79% at concentrations of 4.20, 10.50, 26.25 and 39.375 microg/mL of albiflorin and 0.12 to 2.92% at concentrations of 3.875, 10.85, 23.25 and 58.125 microg/mL of paeoniflorin, and inter-day precision (RSD) was from 1.02 to 1.86% for albiflorin and 0.94 to 3.30% for paeoniflorin, at the same four concentrations. This method was applied in order to analyze albiflorin and paeoniflorin in rat urine following oral administration of traditional Chinese medicinal preparation of Si-Wu decoction.

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Pharmacokinetic study of paeoniflorin in mice after oral administration of Paeoniae radix extract

Cited by 36 publications

References 21 publications

Pharmacokinetics of Paeoniflorin After Oral Administration of Shao-yao Gan-chao Tang in Mice

Pharmacokinetics of Paeoniflorin After Oral Administration of Shao-yao Gan-chao Tang in Mice

Influence of co-administrated sinomenine on pharmacokinetic fate of paeoniflorin in unrestrained conscious rats

Simultaneous determination of albiﬂorin and paeoniﬂorin in rat urine by solid‐phase extraction and high‐performance liquid chromatography following oral administration of Si–Wu decoction

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