Pharmacokinetic Study of .BETA.-Methyldigoxin by Enzyme Immunoassay Using a Novel Specific Antiserum in Rats.

Higashi, Yasuhiko; Watanabe, Naomi; Sasaki, Toshiyuki; Fujii, Youichi

doi:10.1248/bpb.26.247

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…Interference by Dx metabolites may contribute to changes of these parameters. While we could not clearly illustrate factors on a weak change of BA, this phenomenon was consistent with the non-change pattern of BA of h-methyldigoxin by our previous results (Higashi et al, 2003a). Accordingly, the pharmacokinetics of intact Dx are likely to be more precisely expressed by RIA using Antiserum-I.…”

Section: Discussionsupporting

confidence: 80%

Pharmacokinetic interaction with digoxin and glucocorticoids in rats detected by radio-immunoassay using a novel specific antiserum

et al. 2005

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Section: Discussionsupporting

confidence: 80%

Pharmacokinetic interaction with digoxin and glucocorticoids in rats detected by radio-immunoassay using a novel specific antiserum

et al. 2005

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Simultaneous analysis of cardiac glycosides in blood and urine by thermoresponsive LC-MS-MS

et al. 2010

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A new thermoresponsive polymer separation column was applied to simultaneous analysis of four cardiac glycosides (CGs) being widely used for the treatment of arrhythmias and heart failure in human blood and urine. This column is composed of an N-isopropylacrylamide polymer, the surface of which undergoes a reversible alteration from hydrophilic to hydrophobic by changing temperature. The chromatographic separation and retention times can be easily be controlled by adjusting the column temperature. As the column temperature was changed from 50 to 10 °C over 8 min, five CGs, including deslanoside, digoxin, methyldigoxin, digitoxin, and digitoxigenin (internal standard) were better resolved. Using these LC conditions, we analyzed four CGs in human whole blood and urine simultaneously by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Validation data as functions of recovery rates, linearity, accuracy, and precision were carefully tested; all were generally satisfactory. The detection limits for the four CGs in both matrices were 0.2-0.3 ng/mL. The method was applied to analysis of methyldigoxin and its main metabolite digoxin in whole blood and urine samples obtained from a deceased person in actual autopsy case. To our knowledge, this is the first report describing an LC-MS-MS method using a thermoresponsive column for analysis of drug(s). The inclusion of the thermoresponsive column in an LC-MS-MS technique seems to extend the possibility for simultaneous analysis of compounds of different properties, such as hydrophobic precursors and their hydrophilic metabolites in biological samples within limited analysis times.

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Pharmacokinetic Study of .BETA.-Methyldigoxin by Enzyme Immunoassay Using a Novel Specific Antiserum in Rats.

Cited by 2 publications

References 20 publications

Pharmacokinetic interaction with digoxin and glucocorticoids in rats detected by radio-immunoassay using a novel specific antiserum

Pharmacokinetic interaction with digoxin and glucocorticoids in rats detected by radio-immunoassay using a novel specific antiserum

Simultaneous analysis of cardiac glycosides in blood and urine by thermoresponsive LC-MS-MS

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