Pharmacokinetic Characterization of the Postdistribution Phase of Prohormone Atrial Natriuretic Peptides Amino Acids 1–98, 31–67, and Atrial Natriuretic Factor During and After Rapid Right Ventricular Pacing in Dogs

Abstract

Release rate constants and disappearance rate constants were determined for three atrial natriuretic peptides consisting of amino acids 1-98 (i.e., proANF 1-98), the midportion of the ANF prohormone consisting of amino acids 31-67 (i.e., proANF 31-67) and amino acids 99-126 (i.e., ANF) after right ventricular pacing at 100, 125, 150, and 180 bpm in six male mongrel dogs. Right atrial and femoral vein blood was obtained at baseline, and at 5, 12, 19, 26, 56, 86, 116, 146, and 206 minutes after right ventricular pacing. Resulting plasma concentration-time data derived parameters were compared. The disappearance rate constants for atrial and femoral venous proANF 1-98 were 0.0144 +/- 0.0087 (X +/- SD) and 0.0175 +/- 0.0075 min-1, respectively (t = 0.6158) and release rate constants were 0.1569 +/- 0.1504 and 0.0670 +/- 0.0393 min-1, respectively (t = 1.8269; P greater than .05). The proANF 31-67 disappearance rate constants were 0.0139 +/- 0.0082 and 0.0148 +/- 0.0132 min-1, respectively (t = 0.1192) and release rate constants were 0.0957 +/- 0.0414 and 0.1984 +/- 0.1762 min-1, respectively (t = 1.4812). The ANF elimination phase disappearance rate constants were 0.0663 +/- 0.0273 and 0.1116 +/- 0.0539 min-1 (t = 2.0923, P greater than .05), respectively, and the release rate constants were 0.1335 +/- 0.0532 and 0.1638 +/- 0.0520 min-1 (t = 0.7878, P greater than .05), respectively. These data indicate that proANF 1-98 and proANF 31-67 circulating beta post-distribution half-lives are approximately 45 minutes whereas beta half-life of ANF is 10 minutes.(ABSTRACT TRUNCATED AT 250 WORDS)

Kaliuretic peptide and long acting natriuretic peptide as well as atrial natriuretic factor inhibit aldosterone secretion

“…The control subjects received vehicle only, but otherwise adhered to an identical pro¬ tocol of a 1-h equilibration period followed by a 1-h infusion period with a 3-h recovery period of evaluation. One hundred nanograms per kilogram body weight per minute was chosen for the infusion dosage of the atrial natriuretic peptides because the rate of release of the N-terminal ANF prohormone peptides from the atrium of the heart with physiological stimuli is 138-292 ng/kg body wt per min, whereas the release rate of ANF from the atrium is 76 ng/kg body wt per min (Ackerman et al 1992). Molar equivalents of 100 ng/kg body wt dose are 32, 29, 26 and 46 pmol/kg body wt for ANF, proANFs 1-30, 31-67 and 79-98 respectively.…”

Increased Release of Kaliuretic Peptide during Immersion-Induced Central Hypervolemia in Cirrhotic Humans

“…By comparing figures 4 and 5, one observes that pro-ANF 1-98 is doubling, while the kaliuretic peptide concentration is increasing sixfold, and the pro-ANF 69-98 concentration is not significantly increasing with im mersion. Pro-ANF 1-98 has a half-life 15-fold longer than ANF [30], Consequently, clearance from the circulation of pro-ANF 1-98 partially accounts for the observation that when immersion ceases, ANF returned to normal within 1 h, whereas the peptides immunologically recog nized by the kaliuretic peptide radioimmunoassay were still significantly elevated. The FIP-GPC data ( fig.…”

Atrial Natriuretic Hormone, Vessel Dilator, Long-Acting Natriuretic Hormone, and Kaliuretic Hormone Decrease the Circulating Concentrations of Total and Free T4 and Free T3 with Reciprocal Increase in TSH

“…Thus, one group (control group or sham infusion) received only 20 ml normal saline without any of the peptide hormones, and the other four groups received one of the respective peptide hormones in 20 ml normal saline. One hundred nanograms per kg BW/min was chosen for the infusion of these ANPs, because the rate of release of the N-terminal ANH prohormone peptides from the atrium of dog heart with physiological stimuli is 138 -292 ng/kg BW⅐min, whereas the release rate of ANH is 76 ng/kg BW⅐min (15). All subjects were studied in the morning after an overnight fast, beginning their baseline period at 0800 h. Each volunteer was studied in the seated position and received only one peptide hormone infusion.…”