Pharmacokinetic applicability of a validated liquid chromatography tandem mass spectroscopy method for orally administered curcumin loaded solid lipid nanoparticles to rats

Kakkar, Vandita; Singh, Sukhjeet; Singla, Dinesh; Sahwney, Sudhir; Chauhan, Anurag; Singh, Gangandeep; Kaur, Indu Pal

doi:10.1016/j.jchromb.2010.10.017

Cited by 52 publications

(15 citation statements)

References 20 publications

(23 reference statements)

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“…It has been reported that curcumin has many pharmacological functions, such as antioxygenation, antibiosis and antitumor [1][2][3] . Methods such as high-performance thin-layer chromatographic (HPTLC) [4][5][6][7] , high-performance liquid chromatography (HPLC) [8][9][10][11] , capillary electrophoresis with amperometric detection (CE-AD) 12 , matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOFMS) 13 , spectrofluorimetry 14,15 , liquid phase tandem mass spectrometry (LC-MS/MS) 16,17 , 1 H NMR 17 , resonance light scattering technique (RLS) 18 and spectrophotometry [19][20][21] have been employed to determine curcumin in a variety of matrixes. However, most of these methods have low sensitivity, need a large number of organic solvents or metal ions.…”

Section: Curcuminmentioning

confidence: 99%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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A new ionic liquid (IL) based micro-extraction method was developed for the spectrophotometry and spectrofluorometry determination of curcumin in curry products. ILs can effectively extract curcumin from aqueous solution which were used instead of large-scale organic solvents to reduce environmental pollution and production cost. Results show that the fluorescence of curcumin is considerably enhanced in 1-hexyl-3-methylimidazolium hexafluorophosphate ([Hmim][PF 6 ]). Under optimized conditions, the absorbance of curcumin is proportional to the concentration of curcumin in the range of 18.4 ng/mL to 494 ng/mL, and the detection limit is 6.1 ng/mL. In addition, the fluorescence intensity of curcumin is proportional to the concentration of curcumin in the range of 0.050 ng/mL to 120.1 ng/mL, and the detection limit is 0.002 ng/mL. Therefore, an efficient spectrophotometry and spectrofluorometry method for the determination of curcumin was established, and the actual concentration in curry products was satisfactorily determined. The content of curcumin in curry sauce were 14.10±0.40 and 14.32±0.37 mg/g when using the spectrophotometry and spectrofluorometry, the content of curcumin in curry powder were 2.50±1.73 and 2.75±1.40 mg/g when using the spectrophotometry and spectrofluorometry.

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Section: Curcuminmentioning

confidence: 99%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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“…Use of SLNs to improve the bioactivity and bioavailability of various pharmaceuticals including curcumin has been documented in recent years. In this setting, Kakkar et al tried to improve the bioavailability of curcumin by formulating a lipid-based nanoformulation and their findings reveal 39-fold enhancement in its oral bioavailability when incorporated in SLNs to that of native curcumin [87].…”

Section: Solid Lipid Nanoparticlesmentioning

confidence: 99%

“…Their findings suggest a 14-and 2.5-fold enhancement in mean residence time and bioavailability of curcumin, following intravenous administration of curcumin-entrapped MNPs compared with native curcumin in rat model. Kakkar et al initiated to improve the bioavailability of curcumin by synthesizing lipid-based nanoformulations and their findings disclosed 39-fold enhancement in its oral bioavailability when incorporated in SNPs to that of native curcumin [87]. A novel CUR-NS was developed to increase the solubility, dissolution, pharmacokinetics and biodistribution of curcumin [94].…”

Section: Novel Curcumin Nanoformulations With Improved Solubility Andmentioning

confidence: 99%

Emerging role of nanocarriers to increase the solubility and bioavailability of curcumin

Mohanty

Das

Sahoo

2012

Expert Opinion on Drug Delivery

144

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“…[9][10][11][12][13][14][15] Among them one method is reported for estimation of Cur in different organs. Various methods also have been developed for the analysis of Cur in plasma, and urine samples.…”

Section: Introductionmentioning

confidence: 99%

Quantification of curcumin, demethoxycurcumin, and bisdemethoxycurcumin in rodent brain by UHPLC/ESI‐Q‐TOF‐MS/MS after intra‐nasal administration of curcuminoids loaded PNIPAM nanoparticles

Ahmad¹,

Warsi²,

Iqbal³

et al. 2013

Drug Testing and Analysis

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An ultra high performance liquid chromatography-electrospray ionization-synapt mass spectrometric method (UHPLC/ESI-QTOF-MS/MS) for the analysis of curcumin (Cur), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC) in Wistar rat brain homogenate was developed and validated. The chromatographic separation was achieved on a Waters ACQUITY UPLC™ BEH C18 (2.1mm × 100 mm; 1.7μm) column using isocratic mobile phase, consisting of acetonitrile: 10mM ammonium formate: formic acid (90:10:0.05v/v/v), at a flow rate of 0.2 ml min(-1) . The transitions occurred at m/z 367.0694/217.0598, 337.0717/173.0910, 307.0760/187.0844 for Cur, DMC, BDMC and m/z 307.0344/229.0677 for the IS (Nimesulide) respectively. The recovery of the analytes from Wistar rat brain homogenate was optimized using liquid-liquid extraction technique (LLE) in (ethyl acetate: chloform) mixture. The total run time was 3.0 min and the elution of Cur, DMC, BDMC occurred at 1.6, 1.75, 1.70 min, and for the IS 1.87 min, respectively. The linear dynamic range was established over the concentration range of 1.00 ng mL(-1) to 1000.0 ng mL(-1) (r(2) ; 0.9909 ± 0.0011, 0.9911 ± 0.003, and 0.9919 ± 0.0013) for Cur, DMC, and BDMC, respectively. The intra and inter-assay accuracy in terms of % CV for Cur, DMC, and BDMC was in the range 0.47-2.20, 0.47-1.65, and0.44-2.70, respectively. The lower limit of detection (LOD) and quantitation (LOQ) for Cur, DMC, and BDMC were 0.46, 0.05, 0.16 ng mL(-1) and 0.153, 0.015, 0.052 ng mL(-1) , respectively. Analytes were stable and the method proved to be accurate (recovery, >85%), specific and was applied to evaluate the Cur, DMC, BDMC loaded PNIPAM NPs as vehicles for nose to brain drug delivery.

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Pharmacokinetic applicability of a validated liquid chromatography tandem mass spectroscopy method for orally administered curcumin loaded solid lipid nanoparticles to rats

Cited by 52 publications

References 20 publications

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Emerging role of nanocarriers to increase the solubility and bioavailability of curcumin

Quantification of curcumin, demethoxycurcumin, and bisdemethoxycurcumin in rodent brain by UHPLC/ESI‐Q‐TOF‐MS/MS after intra‐nasal administration of curcuminoids loaded PNIPAM nanoparticles

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