Pharmacokinetic and Pharmacodynamic Studies of Four Major Phytochemical Components of Da-Cheng-Qi Decoction to Treat Acute Pancreatitis

Zhao, Jinge; Tang, Wen-Fu; Wang, Jia; Jin, Xiang; Gong, Hanlin; Chen, Guangyuan

doi:10.1254/jphs.13037fp

Cited by 26 publications

(26 citation statements)

References 19 publications

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“…We identified ten components in serum after oral administration of a single dose of DCQD in rats [15]. Some of these components exert anti-inflammatory effects [11], including rhein, magnolol, hesperidin, and naringin, each of which might contribute to regulation of a necrosis-apoptosis switch by DCQD. These findings suggest that rhein is one of the most bioactive components of DCQD [11].…”

Section: Discussionmentioning

confidence: 99%

“…Some of these components exert anti-inflammatory effects [11], including rhein, magnolol, hesperidin, and naringin, each of which might contribute to regulation of a necrosis-apoptosis switch by DCQD. These findings suggest that rhein is one of the most bioactive components of DCQD [11]. Therefore, we investigated the effects of rhein in an attempt to identify the bioactive components of DCQD that underlie its ability to modulate a necrosis-apoptosis switch and thereby alleviate the severity of AP.…”

Section: Discussionmentioning

confidence: 99%

“…Cells were resuspended in 300 μ L binding buffer (10 mM HEPES, pH 7.4, 140 mM NaOH, and 2.5 mM CaCl 2 ) containing 5 μ L annexin V-FITC and 5 μ L propidium iodide (PI) and incubated for 15 min at room temperature in the dark [11, 12]. The suspensions were analyzed by flow cytometry (FAC Scan; Becton Dickinson, USA) to quantitate early apoptotic cells (annexin V-positive and PI-negative) and necrotic or late apoptotic cells (annexin V-positive and PI-positive).…”

Section: Methodsmentioning

confidence: 99%

“…However, the active components and mechanism underlying this effect remain uncertain. We recently showed that many components of DCQD play a key role in an necrosis-apoptosis switch in vitro and that rhein might be the most bioactive component of this medicine [11]. Rhein can promote apoptosis of cancer cells through the mitochondria-dependent pathway [12, 13].…”

Section: Introductionmentioning

confidence: 99%

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Rhein Induces a Necrosis‐Apoptosis Switch in Pancreatic Acinar Cells

Zhao

Zhu

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Objectives. The Chinese herbal medicine Da-Cheng-Qi decoction can regulate a necrosis-apoptosis switch in injured pancreatic acinar cells. This study investigated the effects of rhein, a component of this medicine, on a necrosis-apoptosis switch in pancreatic rat AR42J cells. Methods. Cerulein-treated AR42J cells were used. After pretreatment with 479, 119.8, or 29.9 μg/L rhein, cells were cocultured with rhein and cerulein (10−8 M) for 4, 8, or 16 h. Apoptosis and necrosis were examined using annexin V and propidium iodide costaining. Mitochondria-dependent apoptosis-associated proteins were examined using enzyme-linked immunosorbent assays and western blotting. Results. Few cells died in untreated samples. The number was significantly higher in 16-h-cerulein-treated samples and treatment with 479 μg/L rhein most effectively increased the apoptotic-to-necrotic cell ratio (P < 0.05). In cerulein-treated cells, rhein increased the concentrations of p53, cytochrome C, and caspase-3, and increased the Bax/Bcl-2 ratio in a time- and dose-dependent manner, with the maximum effect in cells treated with 479 μg/L rhein for 16 h (P < 0.05). Conclusions. Rhein induces the necrosis-apoptosis switch in injured pancreatic acinar cells in a time- and dose-dependent manner. Mitochondria-dependent apoptosis signaling pathways might play an important role in this effect.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rhein Induces a Necrosis‐Apoptosis Switch in Pancreatic Acinar Cells

Zhao

Zhu

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Combinations of traditional Chinese and Western treatments are more comprehensive and effective for relieving symptoms than western medicine alone by controlling the course of the disease and reducing mortality [12]. Many studies have investigated the mechanisms of action of various decoctions for the treatment of SAP [13, 14]. Our previous studies found that DCQD significantly improves pathological pancreatic damage in a mouse model of acute necrotizing pancreatitis, inhibits inflammatory reactions, alleviates capillary leakage, and eases the symptoms of SAP-complicated SIRS [15].…”

Section: Introductionmentioning

confidence: 99%

Dachengqi Decoction Attenuates Intestinal Vascular Endothelial Injury in Severe Acute Pancreatitis in Vitro and in Vivo

Pan

Chen

et al. 2017

Cell Physiol Biochem

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Background/Aims: Dachengqi decoction (DCQD) is a well-known traditional Chinese herbal drug with strong anti-inflammatory effects. Angiopoietin-1 (Ang-1) plays a vital role in maintaining the stability and integrity of the vascular wall and prevents vascular leakage due to inflammatory mediators. Our previous work found that DCQD protects against pancreatic injury in rats with severe acute pancreatitis (SAP). This study aims to investigate the effects of DCQD on intestinal endothelial damage in both damaged human umbilical vein endothelial cells (HUVECs) and SAP rats. Methods: HUVECs were randomly divided into four groups: control group, TNF-α group, TNF-α plus Ang-1 group (Ang-1 group), and TNF-α plus DCQD group (DCQD group). Cells were incubated for 6 h, 12 h, and 24 h, before collection. The treatment concentration of DCQD was decided based on a Cell Counting Kit-8 (CCK-8) assay. The monolayer permeability of the HUVECs was assessed by measuring the transendothelial electrical resistance (TEER). Apoptosis was analyzed by flow cytometry. mRNA and protein expression of aquaporin 1 (AQP-1), matrix metalloproteinase 9 (MMP9), and junctional adhesion molecule-C (JAM-C) was evaluated by RT-PCR, immunocytofluorescence, and western blot. Forty male Sprague-Dawley rats were randomized into a control group, SAP group, SAP plus Ang-1 group (Ang-1 group), and SAP plus DCQD group (DCQD group). SAP was induced by intraperitoneal injection of cerulein and lipopolysaccharide (LPS), while the control group received 0.9% saline solution. Evans blue was injected through the penile vein and the rats were then sacrificed 12 h after modeling. Levels of serum amylase, TNF-α, IL-1β, IL-2, and IL-6 were determined by using ELISA. Intestinal tissue was analysed by histology, and capillary permeability in the tissues was evaluated by Evans blue extravasation assay. Protein and mRNA expression of AQP-1, MMP9, and JAM-C were assessed by immunohistofluorescence, western blot, and RT-PCR. Results: DCQD reduced the permeability of HUVEC induced by TNF-α in vitro. Furthermore, DCQD altered the mRNA and protein levels of JAM-C, MMP9, and AQP-1 in HUVECs after TNF-α induction. SAP intestinal injury induced by cerulein combined with lipopolysaccharides was concomitant with increased expression of JAM-C and MMP9, and reduced expression of AQP-1 in intestinal tissue. Pretreatment with DCQD attenuated SAP intestinal injury and lowered the levels of serum amylase, TNF–α, IL-1β, IL-2, and IL-6 effectively. Our study demonstrated that DCQD decreased the expression of JAM-C and MMP9 and increased the expression of AQP-1 both in vitro and in vivo. Conclusion: DCQD can reduce capillary endothelial damage in acute pancreatitis-associated intestinal injury and the mechanism may be associated with the regulation of endothelial barrier function-associated proteins AQP-1, MMP9, and JAM-C.

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Dachengqi decoction alleviates acute lung injury and inhibits inflammatory cytokines production through TLR4/NF‐κB signaling pathway in vivo and in vitro

Liu

2019

J of Cellular Biochemistry

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Background and Objectives: Sepsis that arises from uncontrolled pulmonary inflammation could induce acute lung injury (ALI), leading to the high death rate. Dachengqi decoction (DCQD) is a common traditional Chinese herbal medicine with strong anti-inflammatory effects. The current study aimed to explore the effect of DCQD on the inflammatory cytokines production, the aquaporin-1 (AQP-1) and AQP-5 protein expression in lipopolysaccharide (LPS)-induced ALI models, and the potential mechanisms underlying its effects.Methods: Sprague-Dawley rats and HULEC-5a cells were used as study models in the research. To detect related molecules in the study, the real-time polymerase chain reaction analysis, cell counting kit-8 assay, Western blot analysis, and enzyme-linked immunosorbent assay were performed.Results: DCQD could inhibit the expression of LPS-induced inflammatory cytokines, including interleukin-6 (IL-6), IL-8, and tumor necrosis factor-α (TNF-α), in lung tissues and could reduce pulmonary edema by upregulating the expression of AQP-1 and AQP-5 in rats with LPS-induced ALI. Moreover, the results suggested that the toll-like receptor 4 (TLR4)/NF-κB signaling is indispensable for DCQD to increase the expression of AQP-1 and AQP-5 and inhibits the production of IL-6, IL-8, and TNF-α in LPS-induced HULEC-5a cells. Conclusion:The results of our study suggested that DCQD suppresses the TLR4/NF-κB signaling pathway, increases the protein expression of AQP-1 and AQP-5, and inhibits the production of inflammatory cytokines, by which it may alleviate the inflammatory reactions in ALI and benefit the treatments. K E Y W O R D S acute lung injury (ALI), aquaporin-1 (AQP-1), aquaporin-5 (AQP-5), Dachengqi decoction (DCQD), inflammatory cytokines 1 | INTRODUCTION Acute lung injury (ALI), its severe complication, and pulmonary edema are acute and life-threatening diseases caused by a variety of factors, including trauma, pneumonia, shock, and sepsis; the high mortality rate remains unresolved. 1,2 A major cause of ALI is that the intestinal barrier function of patients with sepsis is often damaged. A large amount of bacteria and endotoxins in the intestinal tract invade the blood circulation through J Cell Biochem. 2019;120:8956-8964. wileyonlinelibrary.com/journal/jcb 8956 |

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Pharmacokinetic and Pharmacodynamic Studies of Four Major Phytochemical Components of Da-Cheng-Qi Decoction to Treat Acute Pancreatitis

Cited by 26 publications

References 19 publications

Rhein Induces a Necrosis‐Apoptosis Switch in Pancreatic Acinar Cells

Rhein Induces a Necrosis‐Apoptosis Switch in Pancreatic Acinar Cells

Dachengqi Decoction Attenuates Intestinal Vascular Endothelial Injury in Severe Acute Pancreatitis in Vitro and in Vivo

Dachengqi decoction alleviates acute lung injury and inhibits inflammatory cytokines production through TLR4/NF‐κB signaling pathway in vivo and in vitro

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