Pharmacognostical Studies of Cistanchis Herba (III) Phylogenetic Relationship of the Cistanche Plants Based on Plastid rps2 Gene and rpl16-rpl14 Intergenic Spacer Sequences.

The sequences of the plastid gene rpl16 and the rpl16-rpl14 spacer region allow discrimination among six species of Scutellaria

“…In an analysis of three species of Cistanche, there were 18 and 14 differences among nucleotide sequences of the rpl16 exon and the rpl16-rpl14 spacer region, respectively, and the frequency of differences in the latter region was also higher than that in the former region (Tomari et al, 2002). In the present study, the frequencies of differences in the two regions were 1.4 and 3.9%, respectively.…”

“…It has been proposed that such sequences might be useful for the identification of higher plants (Nakamura et al, 1997), as demonstrated, for example, in Cistanche (Tomari et al, 2002) and Papaver (Hosokawa et al, 2004). We examined whether these regions might allow discrimination among six species of Scutellaria and, in particular, between Scutellaria galericulata and Scutellaria lateriflora, and whether the differences among sequences might be of diagnostic value.…”

Identification of Cistanche Species by Chemical and Inter-simple Sequence Repeat Fingerprinting

“…Tomari et al has distinguished C. deserticola, C. salsa and C. tubulosa by comparing the nucleotide sequences of the plastid rps2 gene and the intergenic spacer region between rpl16 and rpl14. 12) Jiang et al has developed a HPLC-Diode Array Detector (DAD)-MS fingerprint method to comparatively analyze Cistanche species. 13) In this study, we determined the chemical and genetic distinctions of stems derived from C. deserticola, C. tubulosa, C. salsa and C. sinensis.…”

Studies on Cistanches Herba: A Bibliometric Analysis

“…Molecular phylogenetic analysis provides a good way to distinguish the intrinsic differences between species. The phylogenetic relationships of C. deserticola, C. salsa, and C. tubulosa were revealed by the work of Tu et al using DNA sequencing [82]. The NJ tree constructed from the sequence data showed C. deserticola was closely related to C. salsa, and C. tubulosa was one of the outgroups.…”