Pharmacognostic, physicochemical and chromatographic characterization of Samasharkara Churna

Mukhi, Sangeeta; Bose, Anindya; Panda, Purnendu; Rao, M. M.

doi:10.1016/j.jaim.2015.11.004

Cited by 27 publications

(23 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 30 s, the supernatant was collected by centrifugation at 1500 g for 1 min and used for analysis. The quantitative estimation of organo chlorine, organo phosphorous and pyrethroids was carried out using GC/MS analysis in a DB-5 (30 m × 0.25 mm × 0.25 μm) capillary column (Agilent 7000 Triple Quad GC/MS, USA) [17]. Recovery studies with purified compounds indicated that the overall recovery value was 85%.…”

Section: Methodsmentioning

confidence: 99%

“…Further, clean up was carried out as per the procedures given by API [15]. Aflatoxins were estimated using Waters Alliance 2695 HPLC instrument using a Luna C18 column (Phenomenex) of dimensions 4.6 × 150 mm × 5 μ coupled with Waters 2475 fluorescence detector containing Cobra cell [17]. The excitation wavelength and the emission wavelength for fluorescent detection were set at 362 nm and 455 nm, respectively.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

Telapolu

Kalachavedu

Punnoose³

et al. 2018

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundType 2 Diabetes (T2D) is a polygenic disease requiring a multipronged therapeutic approach. In the current scenario, the use of polyherbals is increasing among the diabetics. MD-1, a poly herbal formulation is constituted as a mixture of six popular anti diabetic herbs, used in the management of Diabetes mellitus (DM). The physicochemical, biochemical and in vitro efficacy studies have been carried out to ascertain the possible mechanisms underlying the anti-diabetic action of MD-1.MethodsMD-1 was evaluated for residual toxins as per Ayurvedic Pharmacoepia of India (API) procedures. The hydro alcoholic extract of the formulation (HAEF) was evaluated for anti oxidant activity against 2, 2-diphenyl-1-picrylhydrazil (DPPH) and nitric oxide radicals in vitro. The effect of HAEF on carbohydrate digestive enzymes α-glucosidase and α-amylase was studied using biochemical assays. HAEF was studied for its glucose lowering potential in L6 myotubes and 3T3L1 preadipocytes, using 2-deoxy-D-[1-3H] glucose (2-DG) uptake assay. Effect of MD-1 on adipogenesis was evaluated in 3T3L1 adipocytes using oil O red staining. The effect of HAEF on mRNA expression of peroxisome proliferator activated receptor gamma (PPARγ) and glucose transporter 4 (GLUT4) in 3T3L1 adiocytes was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). Statistical analysis was performed by student t-test, ANOVA.ResultsResidual toxins present within the API limits and HAEF demonstrated strong antioxidant potential and significantly inhibited the α-glucosidase (IC50 63.6 ± 0.46 μg/mL) and α-amylase (IC50 242.81 ± 1.26 μg/mL) activity. HAEF significantly (p < 0.05) enhanced the insulin stimulated glucose uptake in both the cell lines studied. Unlike standard pioglitazone (PGZ), HAEF modulated the mRNA expression of PPARγ and GLUT4 (p < 0.0001) in 3T3L1 adipocytes, without inducing adipogenesis.ConclusionPhysicochemical parameters established in the study may serve as reference standards in regular quality control. Absence of residual toxins underpins the safety. The enhanced glucose uptake and favorable modulation of insulin sensitivity through a plausible weak PPARγ agonism is similar to the distinct PPARγ activation pattern of several reported natural compound agonists. The differential binding modes of such dynamic combinatorial ligands within the formulation unlike synthetic ligands like thiozolidinediones (TZD) can be linked to the safe mitigation of diabetic complications by MD-1.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

Telapolu

Kalachavedu

Punnoose³

et al. 2018

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…The solutions thus obtained were observed under visible light and UV light. The characteristic colors appeared were recorded 19,20 .…”

Section: Macroscopic and Microscopic Evaluationsmentioning

confidence: 99%

Untitled

2018

IJPSR

View full text Add to dashboard Cite

Polyalthia korinti (Dunal) Benth. & Hook. F. is a rare Indo-Sri Lankan species of Annonaceae family and are traditionally used by tribal people of Ghats region, India for treating several ailments. The number of mature plants and the area of its distribution are too low and this species is placed under the vulnerable critically endangered category. The present study deals with the pharmacognostic and physicochemical evaluations of the leafy part of this plant and was undertaken as an initial step to aid in documentation, quality assurance, proper identification etc. and appropriately to investigate the plant material for its ethanomedicinal claims. Macro and micro morphological parameters of leaf and dried leaves powder were set down following standard procedures recommended by Ayurvedic Pharmacopoeia of India. Macroscopic study of leaf revealed some of the characteristic features like size, shape and color and in microscopic study internal characteristic features like epidermis, cortex, xylem, phloem, trichomes, and stomata were noted. Physicochemical parameters like total ash value, water soluble ash, acid insoluble ash, sulphated ash, foreign organic matter content, moisture content and crude fiber content were determined. Fluorescence analysis with various reagents showed characteristic coloration at day (visible light) and under UV light. Extractive values showed that the methanol soluble phytoconstituents were high compared to other solvents. This study thus lays down the pharmacognostic and physicochemical standardization parameters of the Polyalthia korinti leaf for the first time to serve the identification and quality control purposes.

show abstract

“…Additionally, the usage of herbal drugs has grown incredibly in line with the worldwide trend of folks returning to natural remedies. The increasing utilization of botanicals by the public is forcing moves to evaluate the health claims of these agents and also to establish specifications of quality and manufacture [1] .Herbal drugs exert a key impact in health care programs particularly in developing nations. However hindrance behind the acceptance of herbal medicine in developed nations is the lack of documentation, stringent quality control measures.…”

mentioning

confidence: 99%

Pharmacognostic Standardization of Aralia racemosa L. Stem

Prasanth¹,

Rao²,

Prasad³

2017

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

In accordance with World Health Organization (WHO); traditional medicines are utilized by 70-95% of worldwide population especially in developing countries with regard to their healthcare. Additionally, the usage of herbal drugs has grown incredibly in line with the worldwide trend of folks returning to natural remedies. The increasing utilization of botanicals by the public is forcing moves to evaluate the health claims of these agents and also to establish specifications of quality and manufacture [1] .Herbal drugs exert a key impact in health care programs particularly in developing nations. However hindrance behind the acceptance of herbal medicine in developed nations is the lack of documentation, stringent quality control measures. Therefore, documentation and standardization of the crude materials utilized in herbal medicine is vital for the global compliance of this system of medicine. In this regard, WHO supports the nations to provide conventional remedies and practices in communal and private health services. Construction of monographs are planned fundamentally to promote harmonization in the utilization of herbal drugs with respect to levels of safety, efficacy, and quality control. Pharmacognostic standardization, physicochemical investigation and preliminary phytochemical studies were all over accepted in recognizable proof and confirmation of the genuine plant materials. Standardization of herbal drugs is the strategy of recommending a set of specifications, constant parameters, definitive qualitative and quantitative values, which bring an assurance of quality, efficiency, safety as well as reproducibility. Proper identification and quality assurance of plant materials are essential to ensure reproducible result of herbal medicines, which will add to its safety and efficacy.

show abstract

Pharmacognostic, physicochemical and chromatographic characterization of Samasharkara Churna

Cited by 27 publications

References 21 publications

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

Untitled

Pharmacognostic Standardization of Aralia racemosa L. Stem

Contact Info

Product

Resources

About