Pharmacognostic Evaluation and Determination of Secondary Plant Metabolites by HPTLC and its Antioxidant Activity in Myrica esculenta

Abstract

Introduction:Myrica esculenta Buch.-Ham. (Myricaceae) is commonly known as Box Berry, Kaiphal, and Katphala in Ayurveda. As per the Ayurvedic literature the palnt is used for variety of diseases and disorders. Method: The present study deals with the pharmacognostical standardization, HPTLC analysis and antioxidant activity of methanolict extracts of the leaves of M. esculenta. The plant showed high phenolic and flavonoid content. In-vitro antioxidant study of dried leaves of Myrica esculanta was performed using methanolic extract. Results: Antioxidant activity of M. esculenta methanolic extract showed the least IC 50 value of 60 ± 1.15 μg/ml. Standard ascorbic acid showed an IC 50 value of 2.03 ± 0.06 μg/ml. The calibration curve of Gallic acid showed r 2 of 0.949 and R f of gallic acid was found to be 0.44 ± 0.006. Quantification of gallic acid in the samples of leaves of M. esculenta has been performed and the gallic acid was found to be 0.056%. Conclusion: The presence of gallic acid has not yet been reported and quantified in this species which may be utilized for the proper standardization of the drug. The present study showed new natural antioxidant that can replace the synthetic ones to be used in foods and cosmetics. Key words: Antioxidant, DPPH, Myrica Esculenta, Gallic Acid, HPTLC. anti-microbial, 9,10 anxiolytic, 11 while the fruits are known for their anti-oxidant properties and Antimicrobial nature 12,13 The whole plant is an effective chemo preventive agent in skin. Prashant14 Conventionally, foremost tribal communities of Uttarakhand exploit the bark revealing its ethonobotanical importance. It is well known to be effective in chronic cough, asthma and ear ache. When the bark powder is applied externally, it heals chronic and malignant ulcers, and also to intoxicate the fishes. 15 In Ayurveda, the plant is known to have antispasmodic, antiinflammatory, analgesic properties. 16 The antibacterial activity of fruit and bark of the plant has been performed earlier.17 Till now there are so many Synthetic antioxidants are commercially available but quite unsafe, and their toxicity is a distressing trouble. 18 The natural antioxidants, mainly phenolics and flavonoids, are safe, having prominent biological activity, so the current focuses on natural antioxidants from natural plants. Some Reagents used were from Merk (Germany) and standard gallic acid was procured from Sigma-Aldrich (Steinheim). Chromatographic ConditionsChromatography was performed on Merk HPTLC pre coated silica gel 60GF254 (20 X 20 cm) plates. Methano lic solutions of samples and standard compound gallic acid of known concentrations were applied to the layers as 6 mm-wide bands positioned 15 mm from the bot tom and 15 mm from side of the plate, using Camag Linomat V automated TLC applicator with the nitrogen flow providing a delivery speed of 150 nl/s from the application syringe. These conditions were kept constant throughout the analysis of samples. Detection and Quantification of Gallic acidFollowing sample application, l...

Phytochemical Composition, Antioxidant, and Antimicrobial Attributes of Different Solvent Extracts from Myrica esculenta Buch.-Ham. ex. D. Don Leaves

“…Although the information on phenolic content and the antioxidant role of the fruit extract, juice, jam, and marc of M. rubra [24,25,26] is available, this information is lacking for M. esculenta . Earlier research reported the antimicrobial potential of M. esculenta fruit and bark [23,27,28,29], but still, no research has been carried out on the antimicrobial activity of its leaves. The possible differences in the composition and biological activities between extracts made by using different solvents on the extraction of plant natural products are well described [30].…”

Study on phytochemical, antibacterial and antioxidant properties of Genitiana kurroo Royle

“…The roots and leaves were dried under shade at room temperature. Dried roots and leaves of G. kurroo were finely grounded into a powder using an electrical grinder and stored in airtight containers at 4-8°C for further use (Kumar et al, 2017).…”

“…These solution mixtures were kept in the dark for 30 min, and the optical density was measured at 517 nm using a spectrophotometer against methanol. The control used was 3ml of methanol with 1ml of DPPH solution (0.004%) (Kumar et al, 2017;Bhuyar et al, 2021a, b). The DPPH radical scavenging ability of the sample was determined according to the following equation:…”