Pharmacognostic and Phytochemical Investigations of Stems of Hibiscus micranthus Linn

Kumar, Krishan; Setty, S. Ramachandra; Narsu, Laxmi

doi:10.1016/s0975-3575(10)80074-4

Cited by 15 publications

(13 citation statements)

References 4 publications

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“…In patients with diabetes mellitus, chronic hyperglycemia, in combination with other metabolic disorders, can damage various organ systems, resulting in potentially fatal health complications, the most common of which are retinopathy, nephropathy, neuropathy, and cardiovascular disease [45]. Despite the availability of other therapeutic options, herbal remedies (medicinal plants or their extracts) are highly recommended for the treatment of diabetes since ancient times [44,46]. Alpha-amylase and α-glucosidase are digestion enzymes of carbohydrates which can significantly decrease the postprandial elevation of blood glucose after a carbohydrate diet.…”

Section: Antiobesity Activitymentioning

confidence: 99%

Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits

Al‐Yousef

Alqahtani

Hаssаn

et al. 2021

Journal of Chemistry

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Phytochemical and pharmacological investigations of Elettaria cardamomum L. were mostly focused on its essential oil and organic crude extracts with little attention on its aqueous extracts. Therefore, the current study aimed to investigate the phytochemical profile, in vitro antiobesity, and antidiabetic activities of the aqueous extract of Elettaria cardamomum L. family Zingiberaceae. UPLC-ESI-MS/MS analysis is used for the aqueous extract characterization in both ionization modes. The analysis revealed the tentative identification of forty-seven compounds based on their MS/MS fragmentation pattern and comparison with the reported data. The identified compounds include eight flavones, thirteen phenolic and nonphenolic acids, one coumarin, and nineteen anthocyanins. Moreover, the in vitro antiobesity and antidiabetic activities were also studied. The results showed that E. cardamomum L. aqueous extract inhibits pancreatic lipase and α-amylase enzyme in a concentration-dependent manner, as 1 mg/mL extract was able to inhibit pancreatic lipase and α-amylase by 62.25% ± 0.58 (IC50 = 288.75 ± 1.3 µg/mL) and 70.42% ± 1.5 (IC50 = 220.5 ± 1.3 µg/mL), respectively. Conclusively, the current study indicated that the investigated biological activities of Elettaria cardamomum aqueous extract were attributed to the existence of biologically active metabolites such as flavones, phenolic and nonphenolic acids, coumarins, and anthocyanins. Moreover, it proposed that the aqueous cardamom extract can be used as a natural potential source in different pharmaceutical preparations to protect against variable chronic disorders, especially obesity and diabetes. Deeper in vivo investigations, isolation, purification, and structural elucidation of the major active metabolites from cardamom are recommended.

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Section: Antiobesity Activitymentioning

confidence: 99%

Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits

Al‐Yousef

Alqahtani

Hаssаn

et al. 2021

Journal of Chemistry

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“…4 Suberic acid (4.13%) and its monomethyl ester (2.38%) were also detected by gas chromatography-mass spectrometry (GC-MS) in methanolic extracts of Hibiscus micranthus stem along with 56 other compounds. 5 Furthermore, monomethyl suberate was found by GC-MS in an ethylacetate/hexane extract of Pestalotiopsis JCM2A4, an endophyte growing on Chinese mangrove plant Rhizophora mucronata. 6 tanedioate > potassium tributyltin(IV) octanedioate > potassium dibutyltin(IV) octanedioate > potassium diphenyltin(IV) octanedioate.…”

Section: Introductionmentioning

confidence: 99%

Monomethyl Suberate Screening for Antifungal Activity, Molecular Docking and Drug-Like Properties

Antypenko

Hassan²,

Sadykova³

et al. 2018

ACSi

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Antifungal activity of suberic acid monomethyl ester (monomethyl suberate) was investigated in a growth inhibition assay comprising of 11 different fungi and 3 Phytophthora oomycetes strains relevant in agriculture. In comparison to standard antifungal hymexazol, monomethyl suberate showed moderate antifungal effects at a concentration range of 100-300 µg/mL. Alternaria alternata, Fusarium equiseti, Fusarium fujikuroi and Phytophtora infestans GL-1 were the most sensitive fungi showing inhibition rates up to 100%. Physico-chemical descriptors of monomethyl suberate revealed its low toxicity profile. Molecular docking analysis comprising several known antifungal targets points to the N-myristoyltransferase as the most probable site of interaction.

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“…Literature reveals that H. micranthus possesses a wide range of phytochemicals such as phenolic acids, flavonoids, β-sitosterol, alkanes, fatty alcohols and acids (Jain et al, 1997). Chemical profiling of ethanol extract of H. micranthus roots by GC–MS revealed the presence of seventy nine compounds (Kumar et al, 2010). Available literature revealed that a fingerprint profile has been developed by HPTLC and rutin was analyzed by HPLC in H. micranthus (stem) hydro alcoholic extracts but there is no evidence yet available on the quantitative analysis of biomarkers using validated HPTLC method in H. micranthus extracts.…”

Section: Introductionmentioning

confidence: 99%

Concurrent analysis of bioactive triterpenes oleanolic acid and β-amyrin in antioxidant active fractions of Hibiscus calyphyllus , Hibiscus deflersii and Hibiscus micranthus grown in Saudi Arabia by applying validated HPTLC method

Siddiqui

Al‐Yousef

Al‐Howiriny

et al. 2018

Saudi Pharmaceutical Journal

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In this study, we developed a validated HPTLC method for concurrent analysis of two natural antioxidant triterpenes, oleanolic acid (OA) and β-amyrin (BA) in the biologically active fractions (petroleum ether, toluene, chloroform, ethyl acetate and -butanol) of aerial parts of three species (, and). The chromatography was conducted on normal HPTLC (ready to use glass-plate coated with silica gel 60 F254) plate with chloroform and methanol (97:3, V/V) used as mobile phase. The derivatization of the developed plate was done with p-anisaldehyde and scanned at λ = 575 nm. Well resolved and intense peaks of OA and BA were obtained at Rf = 0.36 and 0.57, respectively. The linear regression equation/correlation coefficient (r) for OA and BA were Y = 6.65x + 553.35/0.994 and Y = 9.177x + 637.23/0.998, respectively in the linearity range of 100-1200 ng/spot indicated good linear relationship. The low values of %RSD for intra-day/inter-day precision of OA (1.45-1.61/1.38-1.59) and BA (1.52-1.57/1.50-1.53) suggested that the method was precise. The recovery/RSD (%) values for OA and BA were found to be 99.21-99.62/1.39-1.95 and 98.75-99.70/1.56-1.80, respectively assures the reasonably good accuracy of the proposed method. Fifteen samples were analyzed to check the content of OA and BA by using the developed HPTLC methods. The content of OA in different samples were found to be 3.87 (HmP) > 1.212 (HcP) > 0.673 (HdC) > 0.493 (HdP) > 0.168 (HdE) > 0.059 (HcC) > 0.015 (HcE) > 0.008 (HmT) µg/mg of the dried weight of extract. However the content of BA was found as: 2.293 (HmP) > 1.852 (HdT) > 0.345 (HdC) > 0.172 (HmT) > 0.041 (HdE) > 0.008 (HcC) µg/mg of the dried weight of extract. Some species fractions exhibited good antioxidant potential like: HcE (IC50 = 17.6 ± 1.8)> HdB (IC50 = 32.16 ± 0.9) > HmP (IC50 = 80.4 ± 4.5) > HmT (IC50 = 99.7 ± 8.2) when compared with ascorbic acid (IC50 = 14.2 ± 0.5), while other fractions exhibited only mild antioxidant capability. The developed HPTLC method can be further exploited for analysis of these markers in the quality assessment of raw material as well as herbal formulations available in the market.

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Pharmacognostic and Phytochemical Investigations of Stems of Hibiscus micranthus Linn

Cited by 15 publications

References 4 publications

Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits

Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits

Monomethyl Suberate Screening for Antifungal Activity, Molecular Docking and Drug-Like Properties

Concurrent analysis of bioactive triterpenes oleanolic acid and β-amyrin in antioxidant active fractions of Hibiscus calyphyllus , Hibiscus deflersii and Hibiscus micranthus grown in Saudi Arabia by applying validated HPTLC method

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