Pharmacognostic and Physico-chemical Investigation of Barleria cristata Linn. (Leaf) for Quality Control Assessment

Joshi, B. C.; Panthri, Neha; Prasad, Nondita; Virk, Jaswinder Kaur

doi:10.18502/pbr.v7i3.7700

Cited by 3 publications

(2 citation statements)

References 9 publications

(11 reference statements)

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“…The microtiter plates were incubated at 37°C with a humidified environment that contained 5% CO 2 after cell inoculation. After 24 h of incubation, Adriamycin (ADR) as positive control and various concentrations (10,20, 40 and 80μg/mL) of test samples (PBPE, PBCE, PBEE, and PBAE) were taken. Then, the absorbance was recorded at test wavelength of (540 nm) on a MicroPlate reader.…”

Section: In Vitro Antiproliferative Activity 241 Cell Viability By Su...mentioning

confidence: 99%

In Vitro Antiproliferative Efficacy, Antioxidant Activity and LC MS Based Metabolite Profiling of Premna Barbata Stem Bark

Joshi,

Juyal,

Sah

et al. 2023

Def. Life. Sc. Jl.

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Premna barbata Wall. ex Schauer is used traditionally as folkloric medicines for the treatment of differentpathological conditions. The first reported constituent from the bark of the plant was an iridoid glycoside premnosidic acid having antioxidant activity. Other species of this genus i.e., Premna latifolia, Premna tomentosa has shown to have antioxidant and cytotoxic activities. Despite the ethnomedicinal uses, no scientific evidence in support of antitumor activity on the stem bark of Premna barbata is reported so far. Hence, the current work aims to assess anticancer potentiality of different extracts of P. barbata on various cancer cell lines. Different extracts i.e., Petroleum ether extract (PBPE), Chloroform extract (PBCE), Ethyl acetate extract (PBEE) and Alcoholic extract (PBAE) were prepared and on each extract in-vitro antiproliferative activity was performed by using SRB assay. The most potent extract i.e., PBEE was then evaluated for antioxidant activity. Qualitative phytochemical investigation of different extracts indicates the presence of proteins, carbohydrates, steroids/triterpenoids, alkaloids, glycosides, flavonoids, and phenolic constituents. Ethyl acetate extract of Premna barbata gives potent cytotoxic activity in all cancer cell lines but more selectively to human colon cancer cell line (COLO-205) with GI 50 44.6 μg/ml. The phenolic and flavonoid content in ethyl acetate extract was 3.43±0.09 mg GE/g and 4.28±0.18mg QE/g respectively. Nineteen compounds were observed in positive (+) ESI mode chromatograms when LC-MS analysis was carried out. The LC-MS analysis by positive ionization mode, the predicted compounds such as Geniposidic acid (synonym: Premnosidic acid) and Rutin were detected. The cytotoxicity observed on cancerous cell lines in our study may be due to the presence of observed compounds. So, it can be concluded that Premna barbata stembark has remarkable cytotoxic activity against different tumor cell lines but the effect is more on colon cell lines as compared to others.

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Section: In Vitro Antiproliferative Activity 241 Cell Viability By Su...mentioning

confidence: 99%

In Vitro Antiproliferative Efficacy, Antioxidant Activity and LC MS Based Metabolite Profiling of Premna Barbata Stem Bark

Joshi,

Juyal,

Sah

et al. 2023

Def. Life. Sc. Jl.

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show abstract

“…Due to the presence of various important secondary metabolites, the medicinal plants need to be explored on an advanced basis which may act as a potential remedy for different diseases globally, mainly in developed countries, but lack of standard quality control measures is one of the major problems in herbal drug acceptability. WHO has been focusing on phytochemical and physicochemical evaluation of crude drugs to develop the quality control profile with standard methods for herbal medicine (Joshi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Pharmacognostic characterization of Dicleptera chinensis by scanning electron microscopy, light microscopy, and analytical techniques

Sarfraz,

Alam,

Din

et al. 2023

Microscopy Res & Technique

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Dicleptera chinensis J. (Acanthaceae) has been employed in traditional medicinal systems for treating various ailments. It has been used as an anti‐inflammatory, wound healing, diuretic, and detoxifying agent in different regions of the world. This study determines several pharmacognostic standards, which are useful to ensure safety, efficacy, and purity of D. chinensis. Different parts of the plant were examined through a scanning electron microscope and light microscope, and cross‐section images revealed several useful botanical features of the plant. The color, size, odor, shape, and surface characteristics of plant parts were also examined macroscopically. Pharmacognostic standardization parameters including ash values, loss on drying, swelling index, hemolytic index, and foaming index were determined in accordance with WHO guidelines. Heavy metal analysis was executed through atomic absorption spectrophotometer which depicted the presence of heavy metals and trace elements within the acceptable range. Qualitative phytochemical tests for alkaloids, flavonoids, saponins, glycosides, tannins, carbohydrates, lipids, protein, and so forth of plant extract were also performed, which showed the valuable amount of these phytochemicals useful for medicinal purposes. Preliminary phytochemical tests provide an indication for major phytoconstituents classes present in the plant. These quantitative and qualitative microscopic features are helpful in establishing the pharmacopeia standards of plant. Assessment of various pharmacognostic features such as morphology of various plant parts explained along with physicochemical and phytochemical analysis could be very helpful for future research.Research Highlights Pharmacognostic standardization is employed as reported evidence for correct identification of D. chinensis. Structures identified by scanning electron microscopy and light microscopy serve as diagnostic features of plant. Important secondary metabolites present in the plant suggest the need for further exploration through advanced metabolomics and other analytical techniques.

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Evaluation of the Bioactive compounds and its Functional role in the Aquatic weed Pistia stratiotes

Varsha,

Saya,

Pankaj

et al. 2024

RJPT

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Pistia stratiotes (L.) is an invasive aquatic weed known as water lettuce, belonging to the Araceae family. It is commonly used for its therapeutic potency against a number of ailments. This study's purpose is to analyze the pharmacognostical characteristics, screening of phytocompounds, fluorescence evaluation and scavenging activity of the leaf powder of P. stratiotes. The acid insoluble ash (3.22±0.17) was found to be less than total ash (21.85±0.19), water-soluble ash (11.18±0.05) and sulphated ash value (9.63±0.35). The hydro-ethanolic extract was fractionated with solvents of different polarity as per increasing order (hexane, chloroform, ethyl acetate, n-Butanol and aqueous). The moisture content of the leaf powder was 1.70±0.01 gm and the extractive value in aqueous (25.106±1.41) was quantitatively more than that of other solvents. The presence of carbohydrates, phenolic substances, alkaloids, gummy materials, tannins, flavonoids, saponins were revealed by phytochemical studies. The n-Butanol fraction of hydro-ethanolic decoction of leaf powder showed 360±0.01 mg GAE/gdry weight for TPC and 680±0.26 mg RE/g dry weight for TFC. The lowest IC50 value for DPPH was observed in n–Butanol fraction of hydro-ethanolic extract of leaf powder extract having 246.826±17.14 µg/ml, showing the highest free radical scavenging activity.

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Pharmacognostic and Physico-chemical Investigation of Barleria cristata Linn. (Leaf) for Quality Control Assessment

Cited by 3 publications

References 9 publications

In Vitro Antiproliferative Efficacy, Antioxidant Activity and LC MS Based Metabolite Profiling of Premna Barbata Stem Bark

In Vitro Antiproliferative Efficacy, Antioxidant Activity and LC MS Based Metabolite Profiling of Premna Barbata Stem Bark

Pharmacognostic characterization of Dicleptera chinensis by scanning electron microscopy, light microscopy, and analytical techniques

Evaluation of the Bioactive compounds and its Functional role in the Aquatic weed Pistia stratiotes

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