Pharmacognostic and free radical scavenging Evaluation of Cyathula prostata (Blume) L.

Abstract

Background: Cyathula prostrata (Blume) L. from the family Amaranthaceae has been used traditionally for rheumatism, dysentery, wounds and urethral discharges in the tropical regions of the world. Aim: The present study was undertaken to perform quality control standardization and to evaluate antioxidant activity of the leaf, stem, root and the whole plant of Cyathula prostrata. Methods: Macroscopic and microscopic evaluations were carried out on the plant using standard procedures. Powdered sample of the leaf was evaluated with various organic solvents for fluorescence. The chloroform, ethyl-acetate and methanolic extracts of the leaf, stem, root and whole plant were subjected to various pharmacognostic analyses and evaluated for in vitro antioxidant activity using DPPH assay.Further, thin layer chromatoghraphy was used to evaluate the chloroform extract. Results: Important epidermal features in the plant include: coastal cells, unbranched, uniseriate, multicellular and non-glandular trichomes. Leaves are amphistomatic showing mostly anomocytic and actinocytic stomata. Starch grains are restricted to the adaxial surface. Vascular bundles are mainly collateral and well-developed bundle sheath. The transverse section of stem is circular, hypodermis (1-3 layers). Cross section of the root is described in detail for the plant. Cortex has angular cells. Fluorescence studies showed different colours. Physico-chemical results are comparable with standards. The TLC profile showed presence of at least seven compounds in the leaf, root and the whole plant extracts, while nine components were obtained from the stem extract. The ethyl acetate extract of the root and ethanol extract of the stem gave the highest phenolic contents (30.09±3.768 mg GAE/g) and DPPH free radical scavenging activity (87.0 ± 0.208), respectively. Conclusion: The distinctive features established in this study are steps in identification, standardization and quality control of this medicinal plant.

Quality assessment and antioxidant study of Pleurotus djamor (Rumph. ex Fr.) Boedijn

“…However, the non-fluorescent compounds often can be converted to fluorescent compounds by applying different reagents. As a result fluorescence analysis displayed an array of colours that could be employed for identification of authentic samples and recognize adulterates (Sonibare et al, 2015;Bhattacharya et al, 2009). In the present study powder drug was treated with 16 different chemical reagents which gave characteristic colour when observed under UV light (254 nm, 365 nm) and was compared with colour detected under visible light (Table 1).…”

Pharmacognostic standardization of a well known edible mushroom, Volvariella volvacea

“…Fluorescence analysis is also an important tool for qualitative assessment of crude drug and it provides an idea about their chemical nature (Sonibare andOlatubosun, 2015, Bhattacharya andZaman, 2009). If substance themselves do not give fluorescence; they may often be converted into fluorescent derivatives or decomposition products by applying different chemical reagents.…”

Study of Quality Assurance For Peumus Boldus M Products By Botanic Profiling, Extraction Optimization, HPLC Quantification And Antioxidant Assay

“…According to the World Health Organization (WHO) the macroscopic and microscopic description of a medicinal plant is the first step towards establishing its identity and purity and should be carried out before any tests are undertaken. 47 The quality control methods are important tool in traditional medicines which serve as useful information for identification, authentication and standardization of herbal medicine. The safety and efficacy of herbal medicines are dependent on the standardization and quality of plant materials.…”