PharmacoGenetic targeting of a C. elegans essential neuron provides an in vivo screening for novel modulators of nematode ion channel function

Analysis of the Impact Hyperglycemia has on Neuronal Functions Using Genetic Approaches in Caenorhabditis elegans

“…Dopamine hydrochloride (Fisher Scientific) was dissolved in deionized distilled water to obtain a 2 mM concentration. 280mL of the 2mM dopamine solution was added to 100mm x 15mm plates containing 10mL of NGM agar and seeded with OP50 (G-animals) and with a 27.75mM glucose solution (G+ animals) to obtain a final dopamine concentration of 56µM.Exogenous methiothepin administration was modified from Calahorro et al(Calahorro et al 2022).Methiothepin mesylate salt (Thermo Fisher Scientific) was dissolved in deionized distilled water to obtain a 50 mM concentration. 280mL of the 50mM methiothepin solution was added to 100mm x 15mm plates containing 10mL of NGM agar and seeded with OP50 (G-animals) and with a 27.75mM glucose solution (G+ animals) to obtain a final serotonin concentration of 1.5µM.…”