Pharmacodynamic Interaction ofQuercus infectoriaGalls Extract in Combination with Vancomycin against MRSA Using Microdilution Checkerboard and Time-Kill Assay

Basri, Dayang Fredalina; Khairon, Radhiah

doi:10.1155/2012/493156

Cited by 20 publications

(18 citation statements)

References 33 publications

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“…Minimum inhibitory concentration (MIC) value of the extracts was determined against the bacterial strains using the two-fold serial microdilution method carried out performed in 96-well microtiter plate according to Basri and Khairon [17]. This procedure will only be performed on the extract that showed inhibitory growth against any tested bacteria in agar well diffusion screening test.…”

Section: Determination Of Mic and Mbc Valuesmentioning

confidence: 99%

Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from Canarium odontophyllum Miq.

Basri¹,

Nor²

2014

AJPS

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Canarium odontophyllum is one of the underutilized fruits among the local community in Sarawak, Malaysia. The leaf extracts from C. odontophyllum (6.25 mg/ml to 50 mg/m) were screened against two Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Extraction of the C. odontophyllum leaves using methanol produced the highest yield (5.46%) followed by water (4.0%) and acetone (2.83%) whereas hexane extracted the lowest yield (0.98%). Out of the four bacterial species tested, only S. aureus was found to be susceptible towards the C. odontophyllum leaves extract. Screening result using agar well diffusion method showed that the antimicrobial activity of the acetone and methanol extracts from C. odontophyllum leaves was concentration-dependent towards the growth inhibition of S. aureus. The MIC values for both MeOH and acetone extracts were 0.391 mg/ml. The MBC value of MeOH extract was twice its MIC value whereas the MBC and MIC values of acetone extract against S. aureus were the same. Phytochemical analysis showed that acetone, MeOH and water extracts contain flavonoid, tannin, terpenoid and phenol. These findings conclude that the leaves of C. odontophyllum may contain therapeutically-useful compounds against S. aureus, which are mostly concentrated in the acetone extract. As such, C. odontophyllum have the potential to be developed as an alternative treatment against Staphylococcus aureus-associated skin and soft tissue infections.

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Section: Determination Of Mic and Mbc Valuesmentioning

confidence: 99%

Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from Canarium odontophyllum Miq.

Basri¹,

Nor²

2014

AJPS

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“…Table shows the results of inoculating the P. aeruginosa 532/14 clinical strain onto a solid growth medium at test time intervals. Literature data regis-ter the synergistic effect of two agents provided that the microbial population is reduced by ≥ 3 log 10 [8].…”

Section: Resultsmentioning

confidence: 99%

Time-Kill Assay: An Efficacy of Synergy Between Carbapenems and Clodronic Acid

Афиногенова

Voroshilova

Афиногенов

et al. 2018

Russian Journal of Infection and Immunity

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Abstract. Currently, a search for augmenting antibiotics activity is still crucial due to elevated frequency of detecting carbapenem-resistant Gran-positive bacterial isolates. To resolve this, it might be reasonable to combine carbapenems metal-â-lactamase (MâL) inhibitors. Unfortunately, no MâL inhibitors approved for treatment of carbapenem-resistant infections are currently available. Pathogenic bacteria may survive antibiotic attack, exert tolerance and persistence accompanied with the ongoing infectious process. In connection with this, determining dependence between antimicrobialrelated bactericidal effect and exposure time on microbes at 4, 8, 12 and 24 hours after the onset, a so called time-kill assay, is necessary. A synergy between both agents was noted upon reduced microbial population by ≥ 3 log10. A checkerboard array followed by seeding the microplate well contents onto a dense nutrient medium at various time points were used to assess a synergistic efficacy of carbapenems applied together with clodronic acid against MâL-producing VIMgenotype P. aeruginosa 532/14 clinical isolate obtained from patients with infectious complications (minimal inhibitory concentrations [MIC] for imipenem or meropenem were 512 μg/ml), microbial burden 106 CFU/ml. Optical density was measured at two wavelengths (490 and 630 nm) in ELx800 reader, within 4–24 hour exposure time to determine time of logarithmic growth phase emerging in test culture. It is noteworthy that magnitude of optical density is a difference between two bichromatic measurements resulting in remarkably reduced inaccuracy due to scratches or fingerprints left on the plate. It was found that clodronic acid exhibited a synergic bactericidal effect with carbapenems against a clinically resistant MâL-producing VIM-genotype P. aeruginosa 532/14 strain. Upon that, imipenem-related antimicrobial activity was evident as early as 8 hours after the onset decreasing cell growth down to 1.4 log10 compared to control, whereas 12 hours later it resulted in total inhibition of test strain by decreasing growth of the test strain by 6 log10. Meropenem in combination with clodronic acid showed a more pronounced activity: complete absence of P. aeruginosa 532/14 growth by 8 hours of incubation, growth suppression by 3.2 log10, which reached 6 log10 12–24 hours after the onset. Time-kill assay allows to identify efficient combinations of carbapenems and MâL inhibitors, which is of great importance for increasing therapeutic efficacy of patients with severe purulent-septic complications.

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“…17 One of the potential plants found in Indonesia is Manjakani seeds (Quercus infectory Gall). [17][18][19][20][21] The seeds have the same ability as other herbal ingredients, and even have a broader ability than some other herbal ingredients. They are popularly carried out to maintain the area of female sex organs and commonly used in dosage form capsules, powders, jelly, sticks or decoction.…”

Section: Introductionmentioning

confidence: 99%

“…These seeds contain secondary metabolites which can inhibit and kill germs or microorganisms that can cause disease in humans. 18,21 Phytochemical examination of ethanol extract showed the presence of phenols, flavonoids, triterpenes, tannins, saponins and alkaloids and several other important minerals. The process of antimicrobial mechanism of Manjakani, namely by interfering with cell wall synthesis, membrane function, protein synthesis, nucleic acid metabolism, and intermediary metabolism, so that bacterial and fungal cells will shrink and overlap lysis.…”

Section: Introductionmentioning

confidence: 99%

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The Effect Of Giving The Rest Cleanser Of Manjakani Decoction Seeds (Quercus Infectoria Gall) Against Leucorrhoea Complaints On Hormonal Contraception Acceptors In The Work Area Of Puskesmas Sukajadi The City Of Bandung

Dewiani¹,

Purnama²

2019

Proceedings of the 1st International Conference on Inter-Professional Health Collaboration (ICIHC 2018)

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The high family involvement in KB (family planning) is an indicator of GERMAS (Healthy Living Community Movement) success which is one of the government's efforts to control the growth of population and improve the qualifying small families. Most family planning programs used by KB acceptors are hormone contraception due to practical, convenient, and reversible work to prevent conception. The use of hormonal contraception is at risk of a 50% increase in vaginal complaints or leucorrhoea compared to women who do not use hormonal contraception. The complaint of leucorrhoea commonly occurs in reproductive girl characterized by an increase in the amount of vaginal mucus, greenish/grayish yellow, itchy, stinging and causing discomfort. Empirically, manjakani is used as a cleanser for female organs to prevent and cure vaginal discharge. It is believed that the active substance contains antibacterial and antifungal effects. This study aims to determine the effect of giving the rest cleanser of manjakani decoction seeds (Quercus infectoria Gall) on vaginal discharge complaints on hormonal contraceptives. The research method uses quasiexperiment with pre-post without control group design. Sampling techniques with executive sampling. The sample in this study were hormonal contraceptive acceptors who had leucorrhoea complaints in the work area of Puskesmas Sukajadi in Bandung City that met the inclusion and exclusion criteria with a total of 26 people who were given a Manjakani seed decoction for seven days as much as two times daily. Measurement of complaints was conducted with a long clinical sheet. Data analysis using nonparametric statistics with the Wilcoxon test. The results of the study found a significant difference in charges before and after giving a manjakani seed decoction with a value of p <0.05. There is a reduction in vaginal discharge complaints by 60%. The administration of Manjakani seed decoction affects the leucorrhoea of hormonal contraceptive acceptors.

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Pharmacodynamic Interaction ofQuercus infectoriaGalls Extract in Combination with Vancomycin against MRSA Using Microdilution Checkerboard and Time-Kill Assay

Cited by 20 publications

References 33 publications

Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from <i>Canarium odontophyllum</i> Miq.

Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from <i>Canarium odontophyllum</i> Miq.

Time-Kill Assay: An Efficacy of Synergy Between Carbapenems and Clodronic Acid

The Effect Of Giving The Rest Cleanser Of Manjakani Decoction Seeds (Quercus Infectoria Gall) Against Leucorrhoea Complaints On Hormonal Contraception Acceptors In The Work Area Of Puskesmas Sukajadi The City Of Bandung

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Pharmacodynamic Interaction ofQuercus infectoriaGalls Extract in Combination with Vancomycin against MRSA Using Microdilution Checkerboard and Time-Kill Assay

Cited by 20 publications

References 33 publications

Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from &lt;i&gt;Canarium odontophyllum&lt;/i&gt; Miq.

Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from &lt;i&gt;Canarium odontophyllum&lt;/i&gt; Miq.

Time-Kill Assay: An Efficacy of Synergy Between Carbapenems and Clodronic Acid

The Effect Of Giving The Rest Cleanser Of Manjakani Decoction Seeds (Quercus Infectoria Gall) Against Leucorrhoea Complaints On Hormonal Contraception Acceptors In The Work Area Of Puskesmas Sukajadi The City Of Bandung

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Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from <i>Canarium odontophyllum</i> Miq.

Phytoconstituent Screening and Antibacterial Activity of the Leaf Extracts from <i>Canarium odontophyllum</i> Miq.