Pharmaco-Toxicological Study of Kageneckia oblonga, Rosaceae

Abstract

The probable antipyretic, antiinflammatory, analgesic and antioxidant properties of Kageneckia oblonga, Rosaceae, were investigated and the major compounds of its active extracts were isolated. The study comprised the acute toxicity of the extracts of global methanol, hexane, dichloromethane and methanol. The cytotoxicity of global methanol extract was studied in three tumoral cell lines. All the extracts exhibited the pharmacological activities under study. Methanol and dichloromethane were the most toxic extracts. From the global methanol extract, isolations were performed of prunasin, 23,24- dihydro-cucurbitacin F, and a new cucurbitacin, 3β-(β-d-glucosyloxy)-16α,23α-epoxycucurbita-5,24-diene-11-one. The cytotoxicity of both cucurbitacins on human neutrophils at the assayed concentrations was not statistically significant. In-vitro assays showed that both cucurbitacins can be partly responsible for the analgesic, antipyretic, and anti-inflammatory activities. Evaluation was done of the cytotoxicity of global methanol extract, 23, 24-dihydrocucurbitacin F, aqueous extracts and prunasin against P-388 murine leukaemia, A-549 human lung carcinoma and HT-29 colon carcinoma. Since global methanol extract presented a strong cytotoxicity against P-388 murine leukaemia, A-549 human lung carcinoma, and HT-29 cell lines, it is highly probable that this extract contain one or more cytotoxic compounds that could be investigated for their potential use as an agent against cancer.

Solidago chilensis Meyen and Kageneckia oblonga Ruiz & Pav.: a minireview on their antioxidant profile

“…Repeated chromatography of methanol extract, the most bioactive extract, followed by crystallization led to the isolation of the known 23, 24-dihydrocucurbitacin F, cucurbitacin 1 (Fig. 2) and prunasin [7].…”

“…Delporte and co-workers [7], considering its traditional use, studied the probable antioxidant properties of Kageneckia oblonga in total methanol, hexane, dichloromethane, methanol, and aqueous extracts. From one of the active extracts, some metabolites were isolated and evaluated through in vitro assays.…”

“…The same authors, Delporte and co-workers [7] studied the probable anti-inflammatory, antipyretic, analgesic effect of Kageneckia oblonga in total methanol, hexane, dichloromethane, methanol and aqueous extracts. Since fever, pain, and inflammation may be mechanistically linked through the formation of intermediates of the arachidonic acid cascade, Kageneckia oblonga extracts were assayed for their probable anti-inflammatory and analgesic properties.…”

Cucurbitacins in plant food

“…Toxicity studies in vitro have indicated that dihydrocucurbitacin F and 3β-(β-D-glucosyloxy)-16α,23α-epoxycucurbita-5,24-dien-11one can be partly responsible for analgesic, antipyretic, and anti-inflammatory activity of Kageneckia oblonga extracts (Delporte et al, 2002).…”

“…For example, some of the compounds have been shown to inhibit haemolysis in vitro (Huang et al, 1998), nitrite production in mouse macrophages (Delporte et al, 2002;Park et al, 2004), and protease (elastase) release in stimulated human neutrophiles (Delporte et al, 2002).…”