Pharmaceuticals from cultured algae

Schwartz, Robert E.; Hirsch, C. F.; Sesin, David F.; Flor, James E.; Chartrain, Michel; Fromtling, Robert E.; Harris, Guy H.; Salvatore, Michael J.; Liesch, Jerrold M.; Yudin, Katherine

doi:10.1007/bf01573860

Cited by 278 publications

(166 citation statements)

References 8 publications

Supporting

Mentioning

164

Contrasting

Unclassified

Order By: Relevance

“…3). This finding is in an agreement with that of Schwartz et al (1990), who isolated cryptophycin-1 from the cyanobacterium Nostoc sp. ATCC 53789 on the basis of its antifungal activity.…”

Section: Discussionsupporting

confidence: 93%

Identification and toxigenic potential of a Nostoc sp.

Nowruzi¹,

Khavari-Nejad²,

Sivonen³

et al. 2012

ALGAE

View full text Add to dashboard Cite

Cyanobacteria are well known for their production of a multitude of highly toxic and / or allelopathic compounds. Among the photosynthetic microorganisms, cyanobacteria, belonging to the genus Nostoc are regarded as good candidate for producing biologically active secondary metabolites which are highly toxic to humans and other animals. Since so many reports have been published on the poisoning of different animals from drinking water contaminated with cyanobacteria toxins, it might be assumed that bioactive compounds are found only in aquatic species causes toxicity. However, the discovery of several dead dogs, mice, ducks, and fish around paddy fields, prompted us to study the toxic compounds in a strain of Nostoc which is most abundant in the paddy fields of Iran, using polymerase chain reaction and liquid chromatography coupled with a diode array detector and mass spectrophotometer. Results of molecular analysis demonstrated that the ASN_M strain contains the nosF gene. Also, the result of ion chromatograms and MS 2 fragmentation patterns showed that while there were three different peptidic compound classes (anabaenopeptin, cryptophycin, and nostocyclopeptides), there were no signs of the presence of anatoxin-a, homoanatoxin-a, hassallidin or microcystins. Moreover, a remarkable antifungal activity was identified in the methanolic extracts. Based on the results, this study suggests that three diverse groups of potentially bioactive compounds might account for the death of these animals. This case is the first documented incident of toxicity from aquatic cyanobacteria related intoxication in dogs, mice, and aquatic organisms in Iran.Key Words: cyanobacteria; natural bioactive compound; Nostoc; toxicity INTRODUCTIONThe genus Nostoc is an ecologically, morphologically, and physiologically diverse genus of microorganisms inhabiting soils, and represents large reservoir of potentially valuable natural compounds (Dembitsky and Řezanka 2005). The ecological significance of the Nostoc species extends beyond the compounds which they primarily known to produce, as many of these organisms are capable of modifying their habitats through the synthesis of biologically active products (Ehrenreich et al. 2005). These compounds demonstrate a diverse range of biological activities and chemical structures, including novel cyclic and linear lipopeptides, fatty acids, alkaloids, and other organic chemicals (Dittmann et al. 2001). A large number of novel antimicrobial agents have been identiReceived September 16, 2012, Accepted November 20, 2012 *Corresponding Author E-mail: bahare77biol@yahoo.com Tel: +98-91-1371-0956, Fax: +98-21-8884-8940 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 304 1,500-2,000 lux for two weeks (Kaushik 1987). Three small fragments of growing co...

show abstract

“…3). This finding is in an agreement with that of Schwartz et al (1990), who isolated cryptophycin-1 from the cyanobacterium Nostoc sp. ATCC 53789 on the basis of its antifungal activity.…”

Section: Discussionsupporting

confidence: 93%

Identification and toxigenic potential of a Nostoc sp.

Nowruzi¹,

Khavari-Nejad²,

Sivonen³

et al. 2012

ALGAE

View full text Add to dashboard Cite

show abstract

“…The cryptophycins are natural products isolated from cyanobacteria and have been found to be highly cytotoxic [1], having strong antifungal [2][3][4][5] and antitumor [6] activities. The structure of cryptophycin 1, the major cryptophycin extracted from Nostoc sp.…”

Section: Introductionmentioning

confidence: 99%

Interaction of cryptophycin 1 with tubulin and microtubules

et al. 1995

Self Cite

View full text Add to dashboard Cite

The cryptophycins are newly discovered antimitotic agents isolated from the eyanobacterium Nostoc. Previous studies using cultured cells demonstrated that microtubules are the target of these compounds. We have studied the interaction of cryptophycin 1 with tubulin and microtubules in vitro. Cryptophycin 1 is an effective inhibitor of tubulin polymerization, causes tubulin to aggregate, and depolymerizes mierotubules to linear polymers somewhat similar to the spiral-like structures produced by the Vinca alkaloids. Cryptophyein I also inhibits vinblastine binding to tubulin but not colchicine binding. Thus, it appears that the cryptophycins may bind to the Vinca site in tubulio or to a site that overlaps with the Vinca site.

show abstract

“…1) is the major cytotoxin in Nostoc sp. 4 ' 5 and displays IC 5 0 values in the pM range. Of special importance is the reduced susceptibility of the cryptophycins to P-glycoprotein mediated multiple drug resistance in comparison to vinblastine, colchicine, and paclitaxel.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Cryptophycin Affinity Labels and Tubulin Labeling

Yang¹,

Georg²

2005

View full text Add to dashboard Cite

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and to the Office of Management and Budget, Paperwork Reduction Project (0704-0188), Washington, DC 20503

show abstract

Pharmaceuticals from cultured algae

Cited by 278 publications

References 8 publications

Identification and toxigenic potential of a Nostoc sp.

Identification and toxigenic potential of a Nostoc sp.

Interaction of cryptophycin 1 with tubulin and microtubules

Synthesis of Cryptophycin Affinity Labels and Tubulin Labeling

Contact Info

Product

Resources

About