Pharmaceutical formulation of HSA hybrid coated iron oxide nanoparticles for magnetic drug targeting

Zaloga, Jan; Pöttler, Marina; Leitinger, Gerd; Friedrich, Ralf P.; Almer, Gunter; Lyer, Stefan; Baum, Eva; Tietze, Rainer; Heimke-Brinck, Ralph; Mangge, Harald; Dörje, Frank; Lee, Geoffrey; Alexiou, Christoph

doi:10.1016/j.ejpb.2016.01.017

Cited by 44 publications

(47 citation statements)

References 39 publications

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“…20 AlbIX solution 10% (w/V) was placed in dialysis bags (MWCO 8 kDa) through a 0.22 µm sterile filter and dialyzed against 4.5 L of ultrapure water (4 water changes, 5 h). The solution was then concentrated to 20 mL using tangential ultrafiltration with an MWCO of 30 kDa.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

“…20 Iron(II) and iron(III) salts were dissolved in ultrapure water. Under constant stirring and argon flow, NH 3 solution 25% was added at 90°C to precipitate the iron oxides.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

“…To address the heterogeneity of nanoparticles and cells, we synthesized 3 different, precisely characterized SPIONs (lauric acid-coated SPIONs 20,21 We investigated their effects on 4 breast cancer cell lines (T-47D, BT-474, MCF7 and MDA-MB-231) belonging to different subtypes, as defined by receptor expression and proliferation rates. 22,23 As a tumor-unrelated control, human umbilical vein endothelial cells (HUVECs) were used, which represent a model system for the study of endothelial cells, one of the first cell types that come in contact with nanoparticles after intravascular application.…”

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confidence: 99%

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Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Poller¹,

Zaloga²,

Schreiber³

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are required. While particles used for imaging should circulate for extended periods of time in the vascular system, SPIONs intended for MDT or hyperthermia should be accumulated in the target area to come into close proximity of, or to be incorporated into, specific tumor cells. In this study, we determined the impact of several accurately characterized SPION types varying in size, zeta potential and surface coating on various human breast cancer cell lines and endothelial cells to identify the most suitable particle for future breast cancer therapy. We analyzed cellular SPION uptake, magnetic properties, cell proliferation and toxicity using atomic emission spectroscopy, magnetic susceptometry, flow cytometry and microscopy. The results demonstrated that treatment with dextran-coated SPIONs (SPION Dex ) and lauric acid-coated SPIONs (SPION LA ) with an additional protein corona formed by human serum albumin (SPION LA-HSA ) resulted in very moderate particle uptake and low cytotoxicity, whereas SPION LA had in part much stronger effects on cellular uptake and cellular toxicity. In summary, our data show significant dose-dependent and particle type-related response differences between various breast cancer and endothelial cells, indicating the utility of these particle types for distinct medical applications.

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Section: Materials and Methods Materialsmentioning

confidence: 99%

“…20 Iron(II) and iron(III) salts were dissolved in ultrapure water. Under constant stirring and argon flow, NH 3 solution 25% was added at 90°C to precipitate the iron oxides.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

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Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Poller¹,

Zaloga²,

Schreiber³

et al. 2017

IJN

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“…For example, Alexiou and co‐workers disclosed a MDT concept based on iron oxide nanoparticles that are modified at the surface with lauric acid and are coated with the protein albumin. The authors demonstrated that this coating allows the upload of chemotherapeutics, such as mitoxantrone, in a non‐covalent manner …”

Section: Introductionmentioning

confidence: 99%

Synthesis of Magnetic‐Nanoparticle/Ansamitocin Conjugates—Inductive Heating Leads to Decreased Cell Proliferation In Vitro and Attenuation Of Tumour Growth In Vivo

Seidel

Balakrishnan

Janko

et al. 2017

Chemistry A European J

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Conjugates based on nanostructured, superparamagnetic particles, at hermolabile linker andacytotoxic maytansinoidw ere developed to serve as am odel for tumour-selective drug delivery and release. It combines chemo-with thermal therapy. The linker-modified toxin was prepared by ac ombination of biotechnology and semisynthesis. Drug release was achieved by hyperthermiat hrough an external oscillating electromagnetic field that induces heat inside the particles. Efficacy of this releasec oncept was demonstrated both for cancerc ell proliferation in vitro, and for tumour growth in vivo, in ax enograft mousem odel. Biocompatibility studies for these magnetic-nanoparticle/ansamitocin conjugates complementthis work.

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“…It is the most abundant protein in plasma and one of the major binders/carriers of drugs that plays an important role in pharmacokinetic fate (absorption, distribution, metabolism, and excretion) and pharmacodynamics (Kratz and Beyer, 1998; Leonis et al, 2015; Alam et al, 2016; Ma et al, 2016; Zaloga et al, 2016). Since the half-life of HSA is about 15–21 days, it cannot be immediately utilized by the human body, which means that exogenous HSA cannot correct hypoproteinemia immediately in cancer patients (Sikuler et al, 2000; Talasaz et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Weakening Impact of Excessive Human Serum Albumin (eHSA) on Cisplatin and Etoposide Anticancer Effect in C57BL/6 Mice with Tumor and in Human NSCLC A549 Cells

et al. 2016

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Excessive human serum albumin (eHSA) impact on anticancer effects is inconsistent. We explored the outcome of cisplatin (DDP)/etoposide (VP-16) plus eHSA in vivo and in vitro. C57BL/6 mice with tumor were used to compare the efficacy of DDP/VP-16 alone and DDP/VP-16+eHSA. Blood albumin was measured to confirm whether eHSA elevate its level. Western blotting assay were used to measure the expression of ERCC1/TOP2A in tumor tissues. Cell proliferation, mRNA, and protein expression of ERCC1/TOP2A were also assayed to compare two groups in A549 cells. Furthermore we evaluated eHSA impact on cell proliferation in RNAi targeting ERCC1/TOP2A in A549 cells, respectively. eHSA reduced the anticancer effect of DDP/VP-16 without altering albumin level, increased protein expression of ERCC1/TOP2A, respectively in mice. Similarly, eHSA increased mRNA and proteins expression of ERCC1/TOP2A in A549 cells. In RNAi A549 cells, however, eHSA no longer weakened but enhanced the anticancer effect of DDP, while no longer altered the effect of VP-16. Our findings suggested that eHSA weaken the anticancer effect of DDP/VP-16 via up-regulating ERCC1/TOP2A expression, respectively. Further molecular mechanism studies are warranted to investigate whether eHSA is not conducive to lung cancer chemotherapy.

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Pharmaceutical formulation of HSA hybrid coated iron oxide nanoparticles for magnetic drug targeting

Cited by 44 publications

References 39 publications

Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Synthesis of Magnetic‐Nanoparticle/Ansamitocin Conjugates—Inductive Heating Leads to Decreased Cell Proliferation In Vitro and Attenuation Of Tumour Growth In Vivo

Weakening Impact of Excessive Human Serum Albumin (eHSA) on Cisplatin and Etoposide Anticancer Effect in C57BL/6 Mice with Tumor and in Human NSCLC A549 Cells

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