Pharmaceutical and biomedical applications of chiral capillary electrophoresis and capillary electrochromatography: An update

Abstract

Pharmaceutical and biomedical applications of chiral capillary electrophoresis and capillary electrochromatography: An updateCapillary electrophoresis is often considered an ideal method for the chiral analysis of compounds due to the high separation power of the technique and has therefore found widespread acceptance for the analysis of drugs and pharmaceuticals. In contrast, capillary electrochromatography is still more or less in an infancy state searching for its place among the analytical separation techniques although interesting applications have been published. The present review summarizes recent developments and applications of chiral pharmaceutical analysis by electromigration techniques published in 2002 and early 2003.

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

“…Due to the vast exploration in this subject, numerous reviews detailing fundamentals and applications can be found in the literature prior to 2005 [6][7][8][9][10][11] and over the past two years [12][13][14][15]. Reviews have also covered methods developed specifically for pharmaceutical compounds [16][17][18][19][20][21][22], biological compounds [23], chiral LC-MS/ CE-MS [24], pesticides [25], pollutants [26], and food additives [27,28].…”

(S)-(+)-2-Octanol as a Chiral Oil Core for the Microemulsion Electrokinetic Chromatographic Separation of Chiral Basic Drugs

“…Since chiral resolution was first described in CE by Zare and co-workers, 1,2 many CE enantioseparation methods have been reported, as previously reviewed. [3][4][5][6][7][8][9][10] These methods are based on several resolution mechanisms, such as ligand-exchange, inclusioncomplexation, ion-pair interactions, micellar electrokinetic chromatography (MEKC) and microemulsion electrokinetic chromatography (MEEKC). MEEKC has several optimization advantages over other techniques in terms of selectivity, 11 elution range and composition.…”

Single‐isomer sulfated α‐cyclodextrins for capillary electrophoresis: Hexakis(2,3‐di‐O‐methyl‐6‐O‐sulfo)‐α‐cyclodextrin, synthesis, analytical characterization, and initial screening tests

“…99%. The structure of intermediate (3) was confirmed by high-resolution MALDI-TOF-MS [12] using a Voyager Elite XL mass spectrometer equipped with delayed extraction capability (Perceptive Biosystems, Framingham, MA, USA), and 1 H NMR, 13 C NMR, double-quantum filtered 1 H-1 H correlation spectroscopy (COSY) and 1 H- 13 C heteronuclear correlation spectroscopy (HETCOR) using Varian UnityPlus spectrometers (300 MHz and 500 MHz, both from Varian, Walnut Creek, CA, USA). Hexakis(2,3-di-O-methyl-6-dimethyl-t-butylsilyl)-a-CD was then converted into intermediate (4), hexakis(2,3-di-O-methyl)-a-CD, by removing the dimethyl-t-butylsilyl protecting group with hydrogen fluoride (HF) in ethanol.…”

“…CE enantiomer separations were last reviewed in the annual enantiomer separations issue of Electrophoresis [1]. That review pointed out that sulfated cyclodextrins [2], including the single-isomer, regioselectively substituted 6-O-sulfo CDs [3][4][5][6][7][8][9][10] were the most widely used resolving agents for the separation of the enantiomers of pharmaceuticals.…”