Temple of Hatshepsut was constructed during the reign of Queen Hatshepsut to worship Idol Amun Rai in the modern era and specifically in the eighteenth dynasty. It is characterized by a unique architectural style and is considered one of the best temples constructed in Deir el-Bahari about 3500 years ago. This temple was built from Thebes limestone extracted from the ancient quarry in Gebel Qurna. Considering the age at which this temple was built and its exposure to severe environmental condition such as temperature change and relative humidity, this building gradually deteriorate with time and cracking and spalling are seen on the surface of the building. This effect of deterioration resulted to structural disintegration and which is ultimately detrimental to the integrity of the masonry. The study therefore aimed at using suspension of Nano fluids synthase from Thebes's limestone in coating the surface of the building to reduce the effect of deterioration, the destruction of its distinctive architectural facades, and the obliteration of its colors. This study further investigates the structural, petrological and mechanical characteristics of the Nano Thebes limestone using the Transmission Electron Microscope, X-Ray Diffraction, and Fourier-transform infrared spectroscope. In addition to this, a number of test were performed which includes the compressive strength test, thermo analytic test (TGA/ DTA) and the microstructural test on the experimental Thebes limestone and the one coated by Nano Synthases. Results of the study ultimately shows that the treatment of Thebes's limestone with Nano fluid synthases enhances the aesthetics, strength, and ability to withstand environmental conditions over thousands of years. More so, preserves the architectural details of the facades and the colors used. Which is the ultimate goal of the study for the purpose of conservation and sustainability of heritage building.