Phakopsora pachyrhizi triggers the jasmonate signaling pathway during compatible interaction in soybean and GmbZIP89 plays a role of major component in the pathway

Barros, Vanessa de Almeida; Fontes, Patrícia Pereira; Souza, Gilza Barcelos de; Gonçalves, Amanda Bonoto; Carvalho, Kênia Mara Baiocchi de; Rincão, Michelle Pires; Lopes, I. de O. N.; Dal-Bianco, Maximiller; Alves, Margareth S.; Marcelino-Guimarães, Francismar Corrêa; Fietto, Luciano Gomes

doi:10.1016/j.plaphy.2020.03.043

Cited by 6 publications

(7 citation statements)

References 37 publications

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“…However, a study of non-host interaction between P. pachyrhizi and A. thaliana has revealed that despite the biotrophic lifestyle of P. pachyrhizi, the pathogen activates marker genes of necrotrophic infection [59,60]. In addition, it was recently demonstrated that P. pachyrhizi triggers the JA pathway during the early and late stages of infection in a susceptible soybean cultivar, demonstrating that P. pachyrhizi mimics a necrotrophic behaviour to promote its development inside the host tissues [61]. It has been suggested that the pathogen direct penetration into the epidermal cells or fungal effectors may modulate the expression of genes aiming the activation of the JA pathway and inhibition of SA defence [61].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it was recently demonstrated that P. pachyrhizi triggers the JA pathway during the early and late stages of infection in a susceptible soybean cultivar, demonstrating that P. pachyrhizi mimics a necrotrophic behaviour to promote its development inside the host tissues [61]. It has been suggested that the pathogen direct penetration into the epidermal cells or fungal effectors may modulate the expression of genes aiming the activation of the JA pathway and inhibition of SA defence [61]. Therefore, it was surprising to observe that the GmCHIT1 promoter was not induced by ethylene precursor and methyl jasmonate analogue treatments assessed in our study.…”

Section: Discussionmentioning

confidence: 99%

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Identification and characterization of a new soybean promoter induced by Phakopsora pachyrhizi, the causal agent of Asian soybean rust

et al. 2021

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Background Phakopsora pachyrhizi is a biotrophic fungal pathogen responsible for the Asian soybean rust disease causing important yield losses in tropical and subtropical soybean-producing countries. P. pachyrhizi triggers important transcriptional changes in soybean plants during infection, with several hundreds of genes being either up- or downregulated. Results Based on published transcriptomic data, we identified a predicted chitinase gene, referred to as GmCHIT1, that was upregulated in the first hours of infection. We first confirmed this early induction and showed that this gene was expressed as early as 8 h after P. pachyrhizi inoculation. To investigate the promoter of GmCHIT1, transgenic soybean plants expressing the green fluorescence protein (GFP) under the control of the GmCHIT1 promoter were generated. Following inoculation of these transgenic plants with P. pachyrhizi, GFP fluorescence was detected in a limited area located around appressoria, the fungal penetration structures. Fluorescence was also observed after mechanical wounding whereas no variation in fluorescence of pGmCHIT1:GFP transgenic plants was detected after a treatment with an ethylene precursor or a methyl jasmonate analogue. Conclusion We identified a soybean chitinase promoter exhibiting an early induction by P. pachyrhizi located in the first infected soybean leaf cells. Our results on the induction of GmCHIT1 promoter by P. pachyrhizi contribute to the identification of a new pathogen inducible promoter in soybean and beyond to the development of a strategy for the Asian soybean rust disease control using biotechnological approaches.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification and characterization of a new soybean promoter induced by Phakopsora pachyrhizi, the causal agent of Asian soybean rust

et al. 2021

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“…Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd., 22, 296-315 could include targeting genes important for pathogen establishment and sustenance or negative regulators of immunity (Garcia-Ruiz et al, 2021). However, to date, only two SBR susceptibility genes, GmbZIP89 (Barros et al, 2020) and GmSPL12I (Qi et al, 2016), described above, have been identified. The identification and manipulation of additional susceptibility factors could provide a novel mode of resistance to the pathogen.…”

Section: Gene Editing Technologiesmentioning

confidence: 99%

Soybean‐Phakopsora pachyrhizi interactions: towards the development of next‐generation disease‐resistant plants

Chicowski,

Bredow,

Utiyama

et al. 2023

Plant Biotechnology Journal

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SummarySoybean rust (SBR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is a devastating foliar disease threatening soybean production. To date, no commercial cultivars conferring durable resistance to SBR are available. The development of long‐lasting SBR resistance has been hindered by the lack of understanding of this complex pathosystem, encompassing challenges posed by intricate genetic structures in both the host and pathogen, leading to a gap in the knowledge of gene‐for‐gene interactions between soybean and P. pachyrhizi. In this review, we focus on recent advancements and emerging technologies that can be used to improve our understanding of the P. pachyrhizi‐soybean molecular interactions. We further explore approaches used to combat SBR, including conventional breeding, transgenic approaches and RNA interference, and how advances in our understanding of plant immune networks, the availability of new molecular tools, and the recent sequencing of the P. pachyrhizi genome could be used to aid in the development of better genetic resistance against SBR. Lastly, we discuss the research gaps of this pathosystem and how new technologies can be used to shed light on these questions and to develop durable next‐generation SBR‐resistant soybean plants.

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“…The resistance induced by rhizospheric growth-promoting microorganisms or necrotrophic pathogens, known as induced systemic resistance (ISR), has jasmonic acid (JA) and ethylene (ET) as the main signaling agents, independent of SA [121][122][123][124]. This is a generalization, since there are already reports where the pathogen Phakopsora pachyrhizi, the causal agent of Asian soybean rust, supposedly modulates the expression of target genes when penetrating the host tissue, activating the JA pathway and inhibiting the defense mediated by SA [125]. It is believed that there is a positive crosstalk between SA, JA, and ET, in addition to gene expression effectors.…”

Section: Induced Resistancementioning

confidence: 99%

“…This is a generalization, since there are already reports where the pathogen Phakopsora pachyrhizi , the causal agent of Asian soybean rust, supposedly modulates the expression of target genes when penetrating the host tissue, activating the JA pathway and inhibiting the defense mediated by SA [ 125 ]. It is believed that there is a positive crosstalk between SA, JA, and ET, in addition to gene expression effectors.…”

Section: Induced Resistancementioning

confidence: 99%

Endophytic Fungi: Biological Control and Induced Resistance to Phytopathogens and Abiotic Stresses

et al. 2021

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Plant diseases cause losses of approximately 16% globally. Thus, management measures must be implemented to mitigate losses and guarantee food production. In addition to traditional management measures, induced resistance and biological control have gained ground in agriculture due to their enormous potential. Endophytic fungi internally colonize plant tissues and have the potential to act as control agents, such as biological agents or elicitors in the process of induced resistance and in attenuating abiotic stresses. In this review, we list the mode of action of this group of microorganisms which can act in controlling plant diseases and describe several examples in which endophytes were able to reduce the damage caused by pathogens and adverse conditions. This is due to their arsenal of molecules generated during the interaction by which they form a kind of biological shield in the plant. Furthermore, considering that endophytic fungi can be an important tool in managing for biotic and abiotic stresses due to the large amount of biologically active substances produced, bioprospecting this class of microorganisms is tending to increase and generate valuable products for agriculture.

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Phakopsora pachyrhizi triggers the jasmonate signaling pathway during compatible interaction in soybean and GmbZIP89 plays a role of major component in the pathway

Cited by 6 publications

References 37 publications

Identification and characterization of a new soybean promoter induced by Phakopsora pachyrhizi, the causal agent of Asian soybean rust

Identification and characterization of a new soybean promoter induced by Phakopsora pachyrhizi, the causal agent of Asian soybean rust

Soybean‐Phakopsora pachyrhizi interactions: towards the development of next‐generation disease‐resistant plants

Endophytic Fungi: Biological Control and Induced Resistance to Phytopathogens and Abiotic Stresses

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