Phagocytotic activity of glial cells in culture

Noske, W; Lentzen, Hans; Lange, Klaus; Keller, Konrad

doi:10.1016/0014-4827(82)90385-8

Cited by 40 publications

(24 citation statements)

References 19 publications

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“…Furthermore, a recent study by Forsby et al (34) could show that human glioma cells in culture had a greatly increased load of secondary lysosomes in postconfluent cultures in contrast to normal glial cells. This could partly be explained as a higher rate of autophagocytosis in the malignant cells (32). In line with this, a high endocytotic activity as well as high content of auto/heterophagosomes and peroxisomes have been found in invasive glioma cells in culture (34)(35).…”

Section: Phagocytosissupporting

confidence: 55%

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Invasiveness of primary brain tumors

Bjerkvig

et al. 1984

Cancer Metast Rev

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Primary malignant neoplasms of the nervous system differ from other types of malignancy in several ways. Clinical progression is due to local invasive growth, while metastases outside the skull are rare. The tumors show no sharp delimitation from the surrounding normal tissue. At the edge, an ill-defined area of invasive tumor cells, reacting glial cells and inflammatory cells is present. At the same time the primary brain tumors are biologically heterogeneous. In this review, a short survey of markers for malignancy in primary brain tumors is given, and some properties of importance for invasive behavior, are listed. These include different cellular enzymes, phagocytotic property, locomotive and proliferative characteristics. Studies of primary brain tumors in situ show invasive growth into the surrounding brain tissue, often followed by hemorrhage and necrosis. In addition spread of tumor cells takes place along preexisting intracranial structures. Recently, several systems for the study of brain tumor invasiveness in culture have been elaborated. Both experimental and human gliomas have been tested. The target tissues include organ culture of embryonic chick heart muscle, chorioallantoic membrane, fetal rat brain tissue and reconstructed vessel walls. It has been shown that glioma cells are able to split junctions between normal cells. They destroy and phagocytose the normal cells and penetrate the normal tissue. The use of brain tissue and reaggregated brain cell cultures as target for glioma cells in culture opens the possibility for an elucidation of invasiveness as one of the most important properties of malignancy in the nervous system.

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Section: Phagocytosissupporting

confidence: 55%

“…It is known that primary cultures of astrocytes from rat brains show phagocytic activity in culture (32,33). Similarly, a high proportion of malignant rat glioma cells in culture show phagocytic activity.…”

Section: Phagocytosismentioning

confidence: 99%

Invasiveness of primary brain tumors

Bjerkvig

et al. 1984

Cancer Metast Rev

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“…[14] In summary, cultured brain astrocytes are highly efficient to accumulate iron from citrate-coated Fe-NP in a time-, concentration-, and temperature dependent manner into intracellular vesicles by a mechanism that is likely to involve endocytosis, a process which is well known to occur in astrocytes in vitro. [29][30][31] However, further studies are required to investigate whether the observed accumulation of Fe-NP in cultured astrocytes is also a property of astrocytes in brain. Since astrocytes have important functions for the iron homeostasis of the brain, [13] the fate of the internalized Fe-NP particles will be interesting to study.…”

Section: Discussionmentioning

confidence: 99%

Advanced Biomaterials Accumulation of Citrate‐Coated Magnetic Iron Oxide Nanoparticles by Cultured Brain Astrocytes

et al. 2010

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Magnetic iron oxide nanoparticles (Fe‐NP) are considered for various applications in the brain. However, little is known so far on the uptake and the metabolism of such nanoparticles in brain cells. Since astrocytes are strategically localized between capillaries and neurons, astrocytes are of particular interest concerning uptake and fate of nanoparticles in the brain. Using astrocyte‐rich primary cultures as model system we have investigated the accumulation of citrate‐coated Fe‐NP by astrocytes. Viable cultured astrocytes accumulate iron from citrate‐coated Fe‐NP in a time‐, concentration‐, and temperature‐dependent manner. The cellular iron content determined after 4 h of incubation increases proportional to the concentration of Fe‐NP, if the particles were applied in concentrations of up to 1000 × 10−6 M of total iron. The iron accumulation from 500 or 1000 × 10−6 M iron as Fe‐NP is significantly slowed by lowering the incubation temperature from 37 to 4 °C. Transmission electron microscopy of the cells revealed that most of the cellular Fe‐NP are present in intracellular vesicles. These data demonstrate that astrocytes accumulate efficiently citrate‐coated Fe‐NP, most likely by an endocytotic pathway.

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“…cells showed phagocytic capacities and were capable of ingesting beads up to 6.4 fxm in size. Moreover the present results indicate that these cells were capable of ingesting larger particles when compared to the capac ity of other cells of the nervous system such as oligodendrocytes and astroglia [Noske et al, 1982] and that such a capacity may be stimulated by known neuritogenic and neuronotrophic agents in vitro: gangliosides [Ledeen, 1984;Massarelli et al, 1985].…”

Section: Discussionmentioning

confidence: 88%

Phagocytosis of Polystyrene Latex Beads by Rat Brain Microglia Cell Cultures Is Increased by Treatment with Gangliosides

et al. 1988

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Cultures of microglial cells were prepared from primary rat brain cultures of astrocytes. Microglia appeared under two main forms: ameboid and ramified. Only the former were capable of ingesting opsonized latex particles of 1 and 6 µm; while the latter, which appeared later in culture, were not capable of phagocytosis. When gangliosides (10^–6M), which are known for their neuronotrophic capacity in vitro, were added to the growth medium (for 20 min) they stimulated the phagocytosis of ameboid microglia 2-fold.

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Phagocytotic activity of glial cells in culture

Cited by 40 publications

References 19 publications

Invasiveness of primary brain tumors

Invasiveness of primary brain tumors

Advanced Biomaterials Accumulation of Citrate‐Coated Magnetic Iron Oxide Nanoparticles by Cultured Brain Astrocytes

Phagocytosis of Polystyrene Latex Beads by Rat Brain Microglia Cell Cultures Is Increased by Treatment with Gangliosides

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