Phagocytosis-dependent and independent mechanisms underlie the microglial cell damage caused by carbon nanotube agglomerates

Shigemoto-Mogami, Yukari; Hoshikawa, Kazue; Hirose, Akihiko; Sato, Kaoru

doi:10.2131/jts.41.501

Cited by 4 publications

(4 citation statements)

References 53 publications

(60 reference statements)

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“…Visualization of Proton Concentration Inside JGOM : The JGOMs were stained in 100 × 10 −6 m HCl solution containing fluorescent dyes (pHrodo Red, 0.1 × 10 −3 m ) for about 1 h (Supporting Information) . Excess dyes were washed out with HCl solution (100 × 10 −6 m ).…”

Section: Methodsmentioning

confidence: 99%

Light‐Driven Active Proton Transport through Photoacid‐ and Photobase‐Doped Janus Graphene Oxide Membranes

et al. 2019

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Biological electrogenic systems use protein‐based ionic pumps to move salt ions uphill across a cell membrane to accumulate an ion concentration gradient from the equilibrium physiological environment. Toward high‐performance and robust artificial electric organs, attaining an antigradient ion transport mode by fully abiotic materials remains a great challenge. Herein, a light‐driven proton pump transport phenomenon through a Janus graphene oxide membrane (JGOM) is reported. The JGOM is fabricated by sequential deposition of graphene oxide (GO) nanosheets modified with photobase (BOH) and photoacid (HA) molecules. Upon ultraviolet light illumination, the generation of a net protonic photocurrent through the JGOM, from the HA‐GO to the BOH‐GO side, is observed. The directional proton flow can thus establish a transmembrane proton concentration gradient of up to 0.8 pH units mm−2 membrane area at a proton transport rate of 3.0 mol h−1 m−2. Against a concentration gradient, antigradient proton transport can be achieved. The working principle is explained in terms of asymmetric surface charge polarization on HA‐GO and BOH‐GO multilayers triggered by photoisomerization reactions, and the consequent intramembrane proton concentration gradient. The implementation of membrane‐scale light‐harvesting 2D nanofluidic system that mimics the charge process of the bioelectric organs makes a straightforward step toward artificial electrogenic and photosynthetic applications.

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Section: Methodsmentioning

confidence: 99%

Light‐Driven Active Proton Transport through Photoacid‐ and Photobase‐Doped Janus Graphene Oxide Membranes

et al. 2019

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“…This response promotes clearance of the pathogen through phagocytosis, and has been shown to result in neurotoxicity and reduced dopamine concentrations in the striatum (19,20). Larger multi-walled carbon nanotubes with carboxylic acid functionalization have previously been found to negatively impact microglial phagocytosis processes (21,22). Therefore, probing the impact of carbon nanotubes on microglia is of critical importance to assess the biocompatibility of SWCNT-based neuro-technologies.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensors

Yang

Yang²,

Bonis-O’Donnell³

et al. 2020

Preprint

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AbstractSingle-walled carbon nanotubes (SWCNT) are used in neuroscience for deep-brain imaging, neuron activity recording, measuring brain morphology, and imaging neuromodulation. However, the extent to which SWCNT-based probes impact brain tissue is not well understood. Here, we study the impact of (GT)6-SWCNT dopamine nanosensors on SIM-A9 mouse microglial cells and show SWCNT-induced morphological and transcriptomic changes in these brain immune cells. Next, we introduce a strategy to passivate (GT)6-SWCNT nanosensors with PEGylated phospholipids to improve both biocompatibility and dopamine imaging quality. We apply these passivated dopamine nanosensors to image electrically stimulated striatal dopamine release in acute mouse brain slices, and show that slices labeled with passivated nanosensor exhibit higher fluorescence response to dopamine and measure more putative dopamine release sites. Hence, this facile modification to SWCNT-based dopamine probes provides immediate improvements to both biocompatibility and dopamine imaging functionality with an approach that is readily translatable to other SWCNT-based neurotechnologies.

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“…22,23 Larger multiwalled carbon nanotubes with carboxylic acid functionalization have previously been found to negatively impact microglial phagocytosis processes. 24,25 Therefore, probing the impact of carbon nanotubes on microglia is of critical importance to assess the biocompatibility of SWCNTbased neuro-technologies.…”

mentioning

confidence: 99%

“…Recognition of tissue damage or pathogenic material causes microglial activation, characterized by a change in cell morphology and an inflammatory response. This response promotes clearance of the pathogen through phagocytosis, and has been shown to result in neurotoxicity and reduced dopamine concentrations in the striatum. , Larger multiwalled carbon nanotubes with carboxylic acid functionalization have previously been found to negatively impact microglial phagocytosis processes. , Therefore, probing the impact of carbon nanotubes on microglia is of critical importance to assess the biocompatibility of SWCNT-based neuro-technologies.…”

mentioning

confidence: 99%

Mitigation of Carbon Nanotube Neurosensor Induced Transcriptomic and Morphological Changes in Mouse Microglia with Surface Passivation

Yang

Bonis-O’Donnell

et al. 2020

ACS Nano

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Single-walled carbon nanotubes (SWCNT) are used in neuroscience for deep-brain imaging, neuron activity recording, measuring brain morphology, and imaging neuromodulation. However, the extent to which SWCNT-based probes impact brain tissue is not well understood. Here, we study the impact of (GT) 6 -SWCNT dopamine nanosensors on SIM-A9 mouse microglial cells and show SWCNT-induced morphological and transcriptomic changes in these brain immune cells. Next, we introduce a strategy to passivate (GT) 6 -SWCNT nanosensors with PEGylated phospholipids to improve both biocompatibility and dopamine imaging quality. We apply these passivated dopamine nanosensors to image electrically stimulated striatal dopamine release in acute mouse brain slices, and show that slices labeled with passivated nanosensors exhibit higher fluorescence response to dopamine and measure more putative dopamine release sites. Hence, this facile modification to SWCNT-based dopamine probes provides immediate improvements to both biocompatibility and dopamine imaging functionality with an approach that is readily translatable to other SWCNT-based neurotechnologies.

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Phagocytosis-dependent and independent mechanisms underlie the microglial cell damage caused by carbon nanotube agglomerates

Cited by 4 publications

References 53 publications

Light‐Driven Active Proton Transport through Photoacid‐ and Photobase‐Doped Janus Graphene Oxide Membranes

Light‐Driven Active Proton Transport through Photoacid‐ and Photobase‐Doped Janus Graphene Oxide Membranes

Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensors

Mitigation of Carbon Nanotube Neurosensor Induced Transcriptomic and Morphological Changes in Mouse Microglia with Surface Passivation

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