Phagocytosis and killing of bacteria in aerobic and anaerobic conditions

Ingham, H. R.; Sisson, P. R.; Middleton, Rosemary L.; Narang, H. K.; Codd, A. A.; Selkon, J. B.

doi:10.1099/00222615-14-4-391

Cited by 75 publications

(22 citation statements)

References 17 publications

(2 reference statements)

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“…Of the strains tested Bacteroides gingivalis gave the greatest inhibition; the inhibitory factor in this strain was to be found in the supernatant of the B. gingivalis culture and seemed to be a heat-stable, low-molecular weight substance. These findings were in contrast to those found with B.fragilis whose inhibitory factor was cell-associated (Ingham et al, 1977(Ingham et al, , 1981Vel et al, 1984).…”

Section: Bacterial Interactions In Mixed Infectionscontrasting

confidence: 85%

Pathogenic synergy: mixed intra-abdominal infections

Maclaren

Namavar

Vught

et al. 1984

Antonie van Leeuwenhoek

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In this article we review our researches into the pathogenesis of mixed infections. These may conveniently be divided into in vitro and in vivo studies. In vitro we confirmed that interference with the killing of aerobes by polymorphonuclear leucocytes (PMN's) is a property of the Bacteroides strains tested and appears to depend on competition for opsonins i.e. complement factors. Further studies are in progress to define which complement factors and which bacterial structures are involved. The influence of B. fragilis on chemotaxis has also been studied. Our preliminary data suggest that B. fragilis is itself poorly chemotactic and reduces the chemoattractivity of Proteus mirabilis. This observation is surprising when we consider that abscess formation is the hall-mark of B. fragilis infections and needs clarification. In vivo we have developed a skin infection model in mice which is economical and gives reproducible and quantitative results. In this model we have demonstrated pathogenic synergy between Escherichia coli and B. fragilis. Further studies are planned to assess the role of complement and bacterial factors in this in vivo synergy.

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Section: Bacterial Interactions In Mixed Infectionscontrasting

confidence: 85%

Pathogenic synergy: mixed intra-abdominal infections

Maclaren

Namavar

Vught

et al. 1984

Antonie van Leeuwenhoek

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“…The phagocytic uptake and killing of several aerobic bacteria such as E. coli and Proteus sp. is diminished by the ability of Bacteroides species to compete for opsonins (Ingham et al, 1981;Jones and Gemmell, 1982;Namavar et al, 1983). Both heat-stable and heat-labile opsonins are depleted from serum incubated with Bacteroides species.…”

Section: Inhibition Of Neutrophil Functionmentioning

confidence: 99%

The Medically Important Bacteroides spp. in Health and Disease

Smith¹,

Rocha²,

Paster³

2006

The Prokaryotes

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“…produced locally as a reaction to invading microorganisms or their components (4,11,21,25). PMNs then phagocytose foreign particles or bacteria (22), discharge their granules' content (2,6), and generate reactive oxygen metabolites (18). These processes are regulated, and the inflammatory response is proportional to the magnitude of the PMN influx (29).…”

mentioning

confidence: 99%

Enzymatic Activities of Bovine Peripheral Blood Leukocytes and Milk Polymorphonuclear Neutrophils during Intramammary Inflammation Caused by Lipopolysaccharide

Prin-Mathieu¹,

Roux

Fauré³

et al. 2002

Clin Vaccine Immunol

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Leukocytes are recruited from peripheral blood into milk as part of the inflammatory response to mastitis. However, excessive accumulation of inflammatory cells alters the quality of milk and the proteases produced by polymorphonuclear neutrophils (PMNs) and macrophages may lead to mammary tissue damage. To investigate PMN recruitment and the kinetics of their intracytoplasmic enzymes in inflammation, we generated mastitis in six cows by intramammary infusion of lipopolysaccharide (LPS). Clinical signs of acute mastitis were observed in all of the cows, and normal status was resumed by 316 h. Intracytoplasmic elastase, collagenase, and cathepsin activities were measured within live cells by flow cytometry in peripheral blood leukocytes and milk PMNs before and during the inflammatory process (at 10 time points between 4 and 316 h). The proportion of immature PMNs was appreciated by CD33 surface labeling measured in flow cytometry. Leukopenia was observed in the peripheral blood 4 h postinfusion, concomitant to an increase in somatic cell counts in milk. CD33؉ PMNs were preferentially recruited from the peripheral blood to milk. Enzymatic activities were detected in PMNs, lymphocytes, and monocytes at levels depending on the cell type, sample nature, and time of collection. Milk PMNs had lower enzymatic activities than peripheral blood PMNs. This study showed that milk PMNs recruited during LPS-induced experimental mastitis have an immature phenotype and significantly lower enzymatic activities than peripheral blood PMNs. This suggests that CD33, an adhesion molecule, may be involved in the egress from blood to milk and that the enzymatic contents of PMNs are partly used during this process.

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Phagocytosis and killing of bacteria in aerobic and anaerobic conditions

Cited by 75 publications

References 17 publications

Pathogenic synergy: mixed intra-abdominal infections

Pathogenic synergy: mixed intra-abdominal infections

The Medically Important Bacteroides spp. in Health and Disease

Enzymatic Activities of Bovine Peripheral Blood Leukocytes and Milk Polymorphonuclear Neutrophils during Intramammary Inflammation Caused by Lipopolysaccharide

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