Phagocytic Activity and Nitric Oxide Production of Circulating Polymorphonuclear Leukocytes from Patients with Peritoneal Carcinomatosis

Baskić, Dejan; Aćimović, Ljubiša; Djukic, Aleksandar; Djurdjević, Predrag; Popović, Suzana; Miličić, Biljana; Labović, Itana; Arsenijević, Nebojša

doi:10.1080/02841860310011087

Cited by 2 publications

(5 citation statements)

References 23 publications

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“…Diminished function of peripheral blood neutrophils has been found in untreated human patients with several different types of cancer 5–12 . In the present study, oxidative burst and phagocytic activities of neutrophils from tumor‐bearing dogs were evaluated because of their importance in host defense against bacterial organisms and possibly defense against tumors 15 .…”

Section: Discussionmentioning

confidence: 99%

“…Most reports evaluating phagocytosis of neutrophils from human cancer patients have simply evaluated the percentage of neutrophils exhibiting phagocytosis and reported the results as normal, 5,6,7 increased, 8 or decreased 11 compared with healthy controls. In 2 of these studies, phagocytic activity was also evaluated and reported as normal 7 or decreased 11 .…”

Section: Discussionmentioning

confidence: 99%

“…Most studies evaluating neutrophil functions of human cancer patients focus on specific types of carcinoma, specifically those of the lung, oral cavity, bladder, colon, rectum, and peritoneal cavity (carcinomatosis) 5–8,10,11 . The experimental methods and results of these studies are varied.…”

Section: Discussionmentioning

confidence: 99%

“…Adult non‐Hodgkin's lymphoma patients receiving chemotherapy also acquire neutrophil function defects 4 . Results of studies evaluating neutrophil function of adult human cancer patients before receiving chemotherapy have been mixed, demonstrating normal, 5–7 increased, 8,9 or decreased 6,7,10–12 phagocytic activity, killing capacity, and/or oxidative burst activities. We are unaware of data describing neutrophil function abnormalities that may contribute to infections or sepsis in dogs with cancer.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of peripheral blood neutrophil function in tumor‐bearing dogs

LeBlanc

Jones

et al. 2010

Veterinary Clinical Pathol

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Background: Peripheral blood neutrophils of untreated human cancer patients have been shown to have normal, increased, and decreased phagocytic activity, killing capacity, and/or oxidative burst activities. Objectives: The objectives of this study were to evaluate oxidative burst and phagocytic activities of peripheral blood neutrophils from tumor‐bearing dogs before therapy and compare them with neutrophil function of healthy control dogs. Methods: Heparinized whole blood was obtained from dogs with high‐grade lymphoma (n=23), sarcoma (n=13), or carcinoma (n=11), and healthy control dogs (n=11) for flow cytometric evaluation of oxidative burst and phagocytic activities. Percentage of bursting cells and amount of oxidative burst activity were determined after stimulation with phorbol 12‐myristate 13‐acetate (PMA) or Escherichia coli. Percentage of phagocytic cells and amount of phagocytic activity were determined after incubation with fluorescent E. coli. Results: Compared with control dogs, dogs with sarcoma (P=.004) and carcinoma (P=.05) had a lower percentage of neutrophils exhibiting oxidative burst activity after stimulation with PMA. Phagocytic activity was significantly lower in dogs with sarcomas compared with control dogs (P<.0001) and dogs with lymphoma (P=.01). Conclusions: Untreated carcinomas and sarcomas in dogs may suppress the percentage of neutrophils capable of oxidative burst when stimulated by PMA. Furthermore, sarcomas also may suppress the amount of phagocytic activity per neutrophil. Until further studies can be performed, the clinical significance of these findings is unknown.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of peripheral blood neutrophil function in tumor‐bearing dogs

LeBlanc

Jones

et al. 2010

Veterinary Clinical Pathol

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“…Підрахунок співвідношення різних видів лейкоцитів у зафарбованих мазках крові проводили стандартним методом за допомогою лейкоцитарного лічильника та мікроскопа (окуляр ×7, об'єктив ×90), попередньо нанісши на скло краплю імерсійної олії. Фагоцитарний індекс визначали за кількістю нейтрофілів, які беруть участь у фагоцитозі Sacharomyces cerevisiae [3].…”

Section: матеріали і методиunclassified

Indicators of immunoreactivity in rats under conditions of different nutrition regimen

Voloshchuk¹,

Bioresources²,

Luchyk³

et al. 2021

Bìol. Tvarin

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The research deals with the integral haematological indices such as markers of immunoreactivity and phagocytic activity of neutrophils in animals kept in conditions of a nutrient imbalance. The animals were divided into four experimental groups: I — animals receiving full-value semi-synthetic ration (control group); II — animals receiving low-protein ration (LPR); III — animals receiving high-sucrose diet (HS); IV — animals receiving low-protein/high-sucrose diet (LPR/HS). It has been found that in animals kept in conditions of nutritional protein deficiency there was a disturbance of the specific immune response, as evidenced by a decrease in the immunoreactivity index and an increase in the index of neutrophils and leukocytes ratio. At the same time, no significant changes in the index of blood leukocytes shift and phagocytic activity of neutrophils were found, indicating the preservation of the non-specific immune response activity. However, in animals of this experimental group compensation of endotoxemia and a decrease in the adaptation index were observed, indicating an inhibition of the adaptive mechanisms. Similar changes in the integral haematological indices were observed in animals kept on a high-sucrose ration. It has been shown that animals consuming a low protein/high-sucrose ration have low immunological reactivity, as evidenced by a 3.4-fold decrease in the immunoreactivity index and a 1.5-fold increase in the blood leukocyte shift index, and disturbances in specific immune response (marker is an increase in the neutrophils and lymphocytes ratio), as well as a significant decrease in the phagocytic index, indicating the ineffectiveness of immune reactions involving neutrophils. At the same time, the intensification of the adaptive mechanisms and a three-fold increase in the reactive neutrophil response index indicates the subcompensation stage of endotoxemia. It is concluded that studied integral haematological parameters can be used as additional early diagnostic markers of impaired immunoreactivity and endotoxemia in animals kept in conditions of different protein and sucrose content in the diet.

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Phagocytic Activity and Nitric Oxide Production of Circulating Polymorphonuclear Leukocytes from Patients with Peritoneal Carcinomatosis

Cited by 2 publications

References 23 publications

Evaluation of peripheral blood neutrophil function in tumor‐bearing dogs

Evaluation of peripheral blood neutrophil function in tumor‐bearing dogs

Indicators of immunoreactivity in rats under conditions of different nutrition regimen

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