Phagocytic Activity and Blood Neutrophils Respiratory Burst State Features Amongst Widespread Purulent Peritonitis Patients in the Postoperative Period Dynamics

Савченко, А. А.; Гвоздев, И. И.; Борисов, А. Г.; Черданцев, Д. В.; Первова, О. В.; Кудрявцев, И. В.; Moshev, A. V.

doi:10.15789/2220-7619-2017-1-51-60

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…Исследование функциональной активности нейтрофилов и моноцитов при заболеваниях органов пищева-рения в настоящее время активно развивается. В одной из работ были обнаружены выраженные изменения продукции АФК нейтрофилами крови у больных гнойным перитонитом [13].…”

Section: таблица 1 характеристики фагоцитарной активности нейтрофилоunclassified

Functional activity of blood phagocytes in opistorchiasis patients

et al. 2019

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Introduction. The urgency of the problem of opistorchiasis is dictated by the long clinical course of the disease with the possibility of formation of hepatobiliary pathology, including cancer. Aim. To study the peculiarities of the production of active oxygen forms of blood phagocytes in patients with opistorchiasis. Material and methods. A total of 42 patients with chronic opisthorchiasis (22 men and 20 women) and 35 apparently healthy patients (18 men and 17 women) between the ages of 24 and 45 were examined. Opistorchiasis was diagnosed by three methods: microscopic examination of duodenal bile, coproovoscopy and serological method. The control group was composed of healthy individuals who underwent routine medical examinations, which included biochemical blood tests, identification of markers for viral hepatitis and antibodies to opistorchs. The culture of neutrophils and blood monocytes was isolated by fractionation of cells on the ficoll with subsequent cleaning from adhering cells. Functional activity of neutrophils and blood monocytes was estimated by chemiluminescence method on production of active oxygen species: basic and after induction with zymosan. Results. In patients with opistorchiasis in comparison with healthy people, in neutrophil granulocytes, the maximum intensity of active oxygen species production decreased and the time to reach the maximum of active oxygen species production was increased both in spontaneous and zymosan-induced reactions in luminoland lucigenin-dependent processes. In monocytes during spontaneous reaction study it was discovered that the maximum intensity of oxygen production in luminoland lucigenindependent processes decreased and the time of reaching the maximum of oxygen production in luminol-dependent process increased in opistorchiasis patients in comparison with healthy people. When studying the zymosan-induced reaction in monocytes, a decrease in the maximum intensity of active oxygen species production in both reactions and an increase in the time to reach the maximum output of active oxygen species in the luminol-dependent process was registered in patients with opistorchiasis in comparison with healthy people. Conclusion. The data obtained indicate low functional activity of blood phagocytes in patients with chronic opistorchiasis in comparison with healthy people.

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Section: таблица 1 характеристики фагоцитарной активности нейтрофилоunclassified

Functional activity of blood phagocytes in opistorchiasis patients

et al. 2019

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“…In the condition of chronic hyperglycemia, which emerges during diabetes mellitus, accumulation of end products of glycosylation and hypoxia intensify the generation of reactive oxygen species, which in turn are a reason of deficiencies of glutathione and superoxide dismutase and development of oxidative stress (Nesterov et al, 2018). Intensity of oxidative stress, as one of the links of pathogenesis of acute generalized peritonitis, derives from high phagocyte activity of neutrophile blood granulocytes, increase in the level of sponta-neous and induced synthesis of secondary reactive oxygen species (Savchenko et al, 2017) and is more pronounced in case of combined pathology (Verveha, 2021) as a manifestation of the syndrome of mutual aggravation.…”

Section: Introductionmentioning

confidence: 99%

“…It has to be noted that lipids are one of the main targets of oxidation damage to reactive oxygen species, because there occurs dysbalance between formation and neutralizations of free-radical compounds (Nesterov et al, 2018). Intensification of free-radical oxidation of lipids leads to progression of the pathological processes, accompanied by lesions of the liver, first of all, as a result of damage to hepatocytes, their edema, ruination of mitochondria and lysosomes, influx of highly toxic products of cellular metabolism into blood and lysosome enzymes (Savchenko et al, 2017;Dalbaşı et al, 2020;Verveha, 2021).…”

Section: Introductionmentioning

confidence: 99%

Oxidative modification of proteins and antioxidant status in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes

Verveha,

Gutyj,

Lishchuk

et al. 2023

Regul. Mech. Biosyst.

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Modern aspects of the pathogenesis of acute inflammation of the peritoneum that is concurrent with diabetes involves analysis of metabolic mechanisms, in particular peroxidaton of proteins – antioxidant defense. Therefore, the objective of our study was to examine the interrelation between the processes of free-radical oxidation of proteins and antioxidant system in the dynamics of development of acute generalized peritonitis against the background of streptozotocin-induced diabetes. The study was performed on 56 non-linear white mature male rats. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin (60 mg/kg). On the 14th day of the development of streptozotocin-induced diabetes, we injected 10% filtrated faeces suspension (0.5 mL) into the abdominal cavity, thus initiating acute generalized peritonitis. Oxidative modification of proteins in blood serum was studied using the I. F. Meshchyshen’s method and the condition of antioxidant protection was monitored according to the activities of superoxide dismutase, catalase, content of reduced glutathione, and the level of ceruloplasmin. The study of the parameters of free-radical oxidation of proteins and study of the condition of antioxidant system in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes demonstrated that oxidative protein modification grew, while the parameters of the activity of antioxidant system were being inhibited, depending on the stage of acute inflammation of the peritoneum. We determined inverse correlation relationships between the products of free-radical oxidation of proteins and parameters of antioxidant system on the third and on the seventh days of modelling of combined pathology. Manifestation of acute generalized peritonitis concurring with streptozotocin-induced diabetes was accompanied by a gradual accumulation of the products of free-radical oxidation of proteins and exhaustion of the antioxidant defense during all stages of the development of acute inflammation of the peritoneum, peaking on the seventh day after administration of faecal suspension (terminal stage of peritonitis). The observed inverse correlations between the levels of oxidative modification of proteins and the activity of superoxide dismutase, catalase, reduced glutathione, and ceruloplasmin on the third and on the seventh days of modelling of combined pathology indicate a predictive role of the processes of free-radical oxidation of proteins in exhaustion of antioxidant-defense resources.

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“…Несмотря на новые разработанные хирургические и медикаментозные методы лечения РГП, летальность при данном заболевании остается высокой, достигая 75,8-100% при развитии различных осложнений, например, сепсиса [1,3,20]. На сегодняшний день доказано, что характер течения РГП, а также механизм развития осложнений определяется не только применяемыми хирургическими методами и качеством послеоперационного лечения, но и зависит от состояния иммунной системы пациента [3,5,27]. Однако роль NK-клеток при РГП до сих пор изучена слабо.…”

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The phenotype of NK-cells in the dynamics of the post-operative period in patients with peritonitis in depending on the outcome of the disease

Савченко

Борисов

Кудрявцев

et al. 2019

Russian Journal of Infection and Immunity

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Our study was aimed at investigating dynamic phenotype pattern of peripheral blood NK cells in patients with widespread purulent peritonitis (WPP) during postoperative period depending on disease outcome. A total of 48 patients aged 30–63 with acute surgical diseases and abdominal injuries complicated by WPP were examined. Blood sampling was performed before surgery (preoperative period) as well as on day 7, 14 and 21 during postoperative period. 40 apparently healthy age-matched subjects were included in control group. Peripheral blood NK cell phenotyping was performed by using flow cytometry with directly immunofluorescently tagged antibodies. Mean fluorescence intensity was measured to estimate expression levels of NK cell surface receptors was measured. It was found that in patients with a favorable WPP outcome during preoperative period the percentage of mature NK cells was decreased that was restored by the end of the postoperative period (21 days post-surgery) due to elevated mature, cytotoxic and cytokine-producing NK cell subsets. In addition, percentage of CD11b-positive NK cell subsets was increased upon favorable outcome by the end of postoperative period as well as frequency of CD57-positive NK cells relative to the preoperative period. However, frequency of mature NK cells with unfavorable WPP outcome vs. control vs. favorable outcome was decreased during preoperative and entire postoperative period. Moreover, amount of cytotoxic NK cells was elevated during examination period upon unfavorable WPP outcome. Further, percentage of mature CD11b-positive NK cells in this patient cohort was decreased during preoperative period and post-surgery. Percentage of CD57-positive NK cells was decreased during entire postoperative period in patients with unfavorable vs. favorable outcome vs. control group. At the same time, patients with unfavorable outcome of this infectious-inflammatory disease were shown to display upregulated expression of CD28 and CD57 markers on NK cells. such features identified in phenotype of peripheral blood NK cells in patients with unfavorable WPP outcome reflect abnormal mechanisms in NK cell maturation and migration, which, in turn, determines disturbance in events regulating acute inflammatory reaction in WPP.

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Phagocytic Activity and Blood Neutrophils Respiratory Burst State Features Amongst Widespread Purulent Peritonitis Patients in the Postoperative Period Dynamics

Cited by 7 publications

References 17 publications

Functional activity of blood phagocytes in opistorchiasis patients

Functional activity of blood phagocytes in opistorchiasis patients

Oxidative modification of proteins and antioxidant status in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes

The phenotype of NK-cells in the dynamics of the post-operative period in patients with peritonitis in depending on the outcome of the disease

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