Phagocytic activities in neonatal monocytes

Speer, C P; Wieland, Marion; Ulbrich, R. G.; Gahr, Manfred

doi:10.1007/bf00439252

Cited by 24 publications

(15 citation statements)

References 22 publications

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“…Chemotaxis of macrophages was evaluated using a modification of the Boyden chamber technique (Celloplex, Basel, Switzerland) as recently described (20). Pooled serum activated by zymosan (50 mg/ml Sigma, Munich, FRG) and FMLP I 0-5 M (Sigma) were used as chemoattractants.…”

Section: Methodsmentioning

confidence: 99%

“…The aliquots were added to ice cold HBSS (450 pl) and the cells immediately centrifuged at 200 x g for 2 min at 40" C and the supernatant was diluted to make agar pour plates for colony counting. Intracellular killing of S. aureus was measured as recently described (20).…”

Section: Methodsmentioning

confidence: 99%

“…Luminol-dependent chemiluminescence was measured using a luminometer (LKB-Wallac 1250); details have been recently published (20). Measurement of 0; and H202.…”

Section: Methodsmentioning

confidence: 99%

“…Release of 0 7 by 1 x lo5 macrophages/ml in suspension was determined by superoxide dismutase inhibitable reduction of cytochrome C using PMA (Consolidated Midland Corporation, Brewster, NY) or opsonized zymosan as stimulus (22). The formation of H202 by macrophages (1 x 105/ml reaction mixture) was assayed by measuring the amount of fluorescence due to the oxidation of homovanillic acid by H202 catalyzed by horseradish peroxidase as recently described (20). Assays were performed in duplicate.…”

Section: Methodsmentioning

confidence: 99%

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Phagocytosis-Associated Functions in Neonatal Monocyte-Derived Macrophages

et al. 1988

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A B S T R A n . Using a Teflon culture system we analyzed different aspects of cell maturation and phagocytic activities in neonatal monocyte-derived macrophages. Morphological and cytochemical characteristics as well as the protein composition of neonatal macrophages were identical with those of adult controls; actin binding protein (265,000 Da), myosin (210,000), a-actinin (102,000) and actin (42,000) could be identified in cells from either source: ~l l phagocytic functions were shown to be perfectly normal in neonatal macrophages when compared with adult cells: random migration, chemotactic response to zymosanactivated serum and formyl-methionyl-leucyl-phenylalanin, ingestion, and killing of Staphylococcus aureus, phagocytosis-associated chemiluminescence, production of oxygen intermediates (superoxide anion, 0;; hydrogenperoxide, H202). Phorbol myristate acetate-stimulated 0;-generation by 1 x lo5 macrophages was 11.8 f 4.7 nmol/h for neonates, and 10.2 f 3.9 for controls; production of H 2 0 2 was 7.6 f 3.5 nmol/h in neonatal macrophages and 6.4 +. In newborns, who suffer an increased susceptibility to serious systemic infections, subnormal phagocyte functions have been described. Neutrophils from neonates showed a decreased deformability (1) and a marked deficiency in movement toward different chemotactic stimuli (2-6). In addition generation of .OH by neonatal neutrophils was shown to be decreased (7). However, neonatal monocytes exhibited basically normal phagocytic functions when compared with adult cells (8). The functions of neonatal macrophages have not been fully explored. Macrophages from newborns synthesize humoral host defense factors such as complement components C2, C4 to a similar extent as phagocytes from adults (9, 10); fibronectin production Received October 12, 1987; accepted April 7, 1988. Correspondence Christian P. Speer, M.D., Universitats-Kinderklinik, RobertKoch-Str. 40, D-3400 Gottingen, Federal Republic of Germany.Supported by Grant Sp 239/2-2 from the Deutsche Forschungsgemeinschaft.2 1 was shown to be decreased in neonatal macrophages (9). Macrophages from newborns who had been activated by interferon-7 effectively killed or inhibited replication of toxoplasma gondii (1 I), presentation of bacterial Ag, and production of interleukin 1 was normal in cord blood monocytes/macrophages (12, 13). However, it has been suggested, that the decreased production of interferon-y by human neonatal cells (13) could be due to a functionally immature macrophage (14). Our study was initiated to examine the principal phagocyte activities of monocyte-derived macrophages in response to inflammation, i.e. chemotaxis, phagocytosis, killing, and generation of oxygen metabolites. Momholonical studies included a detailed analysis of the protein compos~ion of macrophages. MATERIALS AND METHODS Cell separation. Umbilical venous blood was withdrawn fromthe placenta into heparin (1-20 U/ml) immediately after delivery of healthy term newborns who were products of an uncomplicated pregnancy, normal labor, and...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Luminol-dependent chemiluminescence was measured using a luminometer (LKB-Wallac 1250); details have been recently published (20). Measurement of 0; and H202.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Phagocytosis-Associated Functions in Neonatal Monocyte-Derived Macrophages

et al. 1988

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“…Phagocytosis and intracellular killing by neonatal monocytes are comparable to those achieved by adult MNCs (25)(26)(27)(28)(29). However, these data are derived from stimulation studies using early-onset sepsis pathogens or latex particles; there are no analogous data on phagocytosis of SE by monocytes in preterm infants.…”

Section: Monocyte Surface Expression Of Tlrsmentioning

confidence: 99%