Phagocyte activity in the peripheral blood of pregnant women with systemic lupus erythematosus and in the cord blood of their newborns

Сухих, Г. Т.; Safronova, V. G.; Van'ko, L. V.; Matveeva, N. K.; Belyaeva, Anastasiya S.; Fedorova, E. V.; Nikolaeva, M. A.; Клименченко, Н И; Krechetova, Krechetova L.V.

doi:10.1111/1756-185x.13085

Cited by 1 publication

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“…Consistent with our present observations, circulating leukocytes from pregnant women presented reduced migration index and reduced H 2 O 2 production compared with non‐pregnant controls 36 . Regarding monocyte efferocytosis capacity during pregnancy, pregnant women monocytes are less efficient than monocytes from non‐pregnant controls and the reduced efferocytic capacity was even more pronounced in patients with preeclampsia 37 or systemic lupus erythematosus 38 …”

Section: Discussionsupporting

confidence: 90%

“…*P < .05 of eight different placentas reduced efferocytic capacity was even more pronounced in patients with preeclampsia 37 or systemic lupus erythematosus. 38 Apoptotic cell clearance is deeply associated with tissue repair mechanisms which are central to homeostasis maintenance as well. 19,21,39 VIP conditioning of monocytes favoured these events in the decidua.…”

Section: Discussionmentioning

confidence: 99%

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Decidual factors and vasoactive intestinal peptide guide monocytes to higher migration, efferocytosis and wound healing in term human pregnancy

et al. 2020

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Aim: To explore the functional profile of circulating monocytes and decidual macrophages at term human pregnancy and their contribution to tissue repair upon stimulation ex vivo with decidual factors and the vasoactive intestinal peptide (VIP). Methods: Peripheral blood monocytes were isolated from pregnant and non-pregnant volunteers and tested in vitro with decidual explants from term placenta and VIP. The effect of VIP on decidual explants and the effect of its conditioned media on monocytes or decidual macrophages isolated by magnetic beads was carried out by RT-qPCR and ELISA for cytokines expression and release. Migration assays were performed in transwell systems. Efferocytosis was assessed in monocytes or decidual macrophages with CFSE-labelled autologous apoptotic neutrophils and quantified by flow cytometry. Monocyte and decidual macrophages wound healing capacity was evaluated using human endometrial stromal cell monolayers. Immunohistochemistry was performed in serial tissue sections of different placentas. Results: VIP is expressed in the villi as well as in trophoblast giant cells distributed within the decidua of term placenta. VIP induced the expression of antiinflmammatory markers and monocyte chemoattractant CCL2 and CCL3 in decidual tissues. Monocytes presented higher migration towards decidual explants than CD4 and CD8 cells. VIP-conditioned monocytes displayed an enhanced efferocytosis and wound healing capacity comparable to that of decidual macrophages. Moreover limited efferocytosis of pregnant women monocytes was restored by VIP-induced decidual factors. Conclusion: Results show the conditioning of monocytes by decidual factors and VIP to sustain processes required for tissue repair and homeostasis maintenance in term placenta.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Decidual factors and vasoactive intestinal peptide guide monocytes to higher migration, efferocytosis and wound healing in term human pregnancy

et al. 2020

View full text Add to dashboard Cite

show abstract

Phagocyte activity in the peripheral blood of pregnant women with systemic lupus erythematosus and in the cord blood of their newborns

Abstract: Pregnant SLE women have low ingesting capacity of phagocytes. Suppression of phagocytosis in their newborns is mainly due to reduced number of cells producing ROS.

Cited by 1 publication

References 43 publications

Decidual factors and vasoactive intestinal peptide guide monocytes to higher migration, efferocytosis and wound healing in term human pregnancy

Decidual factors and vasoactive intestinal peptide guide monocytes to higher migration, efferocytosis and wound healing in term human pregnancy

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