Phagemid Vectors for Phage Display: Properties, Characteristics and Construction

Qi, Huan; Lu, Haiqin; Qiu, Hua‐Ji; Petrenko, Valery A.; Liu, Aihua

doi:10.1016/j.jmb.2012.01.038

Cited by 130 publications

(88 citation statements)

References 94 publications

(69 reference statements)

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“…More than 40 type III and 10 type VIII phagemids have been constructed during the past 20 years for different applications of phage display technology [50]. The phage coat proteins pVII and pIX can also be used to display foreign proteins [51].…”

Section: Discussionmentioning

confidence: 99%

Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase

et al. 2013

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“…Lower amounts of glucose results in background expression of pIII fusions and clones with growth advantages (e.g., truncated scFvs) might overgrow leading to a reduced library diversity. More detailed information about antibody phage display [24] and commonly used phagemid vectors can be found elsewhere [25].…”

Section: Preliminary Notesmentioning

confidence: 99%

Detailed Protocols for the Selection of Antiviral Human Antibodies from Combinatorial Immune Phage Display Libraries

Diebolder¹,

Krawczyk²

2018

Antibody Engineering

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Broadly, neutralizing antiviral antibodies holds great promise for improving treatment opportunities for patients suffering from viral infections (e.g., human immunodeficiency virus [HIV], hepatitis B virus [HBV], cytomegalovirus [CMV], Rabies, Ebola, Zika) leading to serious health disorders or even to death without effective antiviral treatment. The potential of antibodies in host protection against lethal viral infections has been demonstrated in numerous animal models and is best exemplified by the protection conferred to neonates by maternal antibodies. Over the past few decades, virus-neutralizing human monoclonal antibodies (nAbs) have been isolated from humans successfully cured of disease using a wide range of recently developed antibody isolation technologies. In this chapter, we present an approach for isolating recombinant human nAbs from combinatorial gene libraries being cloned from individuals who have recovered from viral infections. The presented protocols describe the selection and screening of antiviral single-chain antibody fragments (scFvs) from phage display immune libraries. This technology represents a well-established, high-throughput approach allowing fast selection of broadly neutralizing, antiviral antibodies. The protocols for generating and selecting antigen-specific scFvs can be applied for the selection of scFvs against any target.

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“…To study effects of nanomedicine targeting in vitro and in vivo, we developed a toolkit based on the filamentous phage fd-tet [70]. The whole architecture and function of the phage particles have been engineered through the reconstruction of their DNA [30,70,71]. The majority of phage display projects use p3-displayed libraries to discover high-affinity peptides and antibodies using affinity selection technique (biopanning) [30] (Figure 2A).…”

Section: Promiscous Peptides and Proteins: Potential Navigating Sensorsmentioning

confidence: 99%

Autonomous self-navigating drug-delivery vehicles: From Science Fiction to Reality

Petrenko

2017

Ther. Deliv.

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Low efficacy of targeted nanomedicines in biological experiments enforced us to challenge the traditional concept of drug targeting and suggest a paradigm of 'addressed self-navigating drug-delivery vehicles,' in which affinity selection of targeting peptides and vasculature-directed in vivo phage screening is replaced by the migration selection, which explores ability of 'promiscuous' phages and their proteins to migrate through the tumor-surrounding cellular barriers, using a 'hub and spoke' delivery strategy, and penetrate into the tumor affecting the diverse tumor cell population. The 'self-navigating' drug-delivery paradigm can be used as a theoretical and technical platform in design of a novel generation of molecular medications and imaging probes for precise and personal medicine. The first medical nanovehicle was portrayed in the science fiction movie 'Fantastic voyage'. It was a micron-size submarine navigating by a 'miniaturized' pilot along the patient's vasculature and boarded with a crew of sergeants aimed to destroy a clot in the patient's brain. Some characteristic features of the fantastic microsubmarine resemble desired vital technical characteristics of modern medical nanodevices. They include: autonomous self-navigation in the bloodstream and tissue, a means for destruction of biological barriers and tumor-surrounding threat cells, traceability for imaging of the nanovehicle location and capability to deliver a molecular cargo to the site of disease. Appearance of drug-delivery nanovehicles (nanomedicines) provoked a new impulse toward development of targeted cancer chemotherapeutics. The first generation of nanoparticle therapeutics were autonomous non-navigating vehicles [1]. They were proposed to take advantage of abnormalities in tumor vasculature for accumulation of drugs in tumor microenvironment exploring enhanced permeability and retention (EPR) effect [2,3]. In attempts to enhance their therapeutic efficacy, the second generation of nanomedicines explored the Ehrlich's 'magic bullet' concept for the nanomedicine targeting toward the vasculature and tumor cells [4]. Discovery of unique, organ-specific vasculature receptors, called 'zip codes', suggested a new approach for organ-and tumor-targeted drug delivery, harnessing the power of the in vivo phage display technique -the concept developed by Pasqualini, Ruoslahti and colleagues [4][5][6][7][8][9][10]. Since tumors were considered as a monocellular pathology, majority of anticancer therapeutics were designed to target exclusively cancer cells or cells of tumor vasculature [1]. The concept of tumor-targeted nanomedicines has been around for years, but it has been underdeveloped because mostly relied on the assumed homogeneity of tumor cells and their proximity to the defective tumor epithelium, as illustrated in Figure 1. The attempts of adapting Ehlich's ideas of direct tumor targeting have shown insignificant improvements [11]. Analysis of nanoparticle delivery data accumulated during the past decade demonstrated that only a tiny ...

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Phagemid Vectors for Phage Display: Properties, Characteristics and Construction

Cited by 130 publications

References 94 publications

Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase

Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase

Detailed Protocols for the Selection of Antiviral Human Antibodies from Combinatorial Immune Phage Display Libraries

Autonomous self-navigating drug-delivery vehicles: From Science Fiction to Reality

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