Phage therapy: present and future

Kolesnikova, S G; Tulyakova, E; Moiseeva, I. Ya.

doi:10.1088/1742-6596/784/1/012027

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…In recent years, the emergence of antibiotic-resistant pathogenic bacteria has become a crucial health problem worldwide. Lytic phages have been considered as potential antimicrobial agents to reduce antibiotic-resistant issues [ 8 , 9 ]. Phage-based biocontrol of bacterial pathogens has been applied in several areas, including food processing, agriculture, and aquaculture [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Two New Shiga Toxin-Producing Escherichia coli O103-Infecting Phages Isolated from an Organic Farm

et al. 2021

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Shiga toxin-producing Escherichia coli (STEC) O103 strains have been recently attributed to various foodborne outbreaks in the United States. Due to the emergence of antibiotic-resistant strains, lytic phages are considered as alternative biocontrol agents. This study was to biologically and genomically characterize two STEC O103-infecting bacteriophages, vB_EcoP-Ro103C3lw (or Ro103C3lw) and vB_EcoM-Pr103Blw (or Pr103Blw), isolated from an organic farm. Based on genomic and morphological analyses, phages Ro103C3lw and Pr103Blw belonged to Autographiviridae and Myoviridae families, respectively. Ro103C3lw contained a 39,389-bp double-stranded DNA and encoded a unique tail fiber with depolymerase activity, resulting in huge plaques. Pr103Blw had an 88,421-bp double-stranded DNA with 26 predicted tRNAs associated with the enhancement of the phage fitness. Within each phage genome, no virulence, antibiotic-resistant, and lysogenic genes were detected. Additionally, Ro103C3lw had a short latent period (2 min) and a narrow host range, infecting only STEC O103 strains. By contrast, Pr103Blw had a large burst size (152 PFU/CFU) and a broad host range against STEC O103, O26, O111, O157:H7, and Salmonella Javiana strains. Furthermore, both phages showed strong antimicrobial activities against STEC O103:H2 strains. The findings provide valuable insight into these two phages’ genomic features with the potential antimicrobial activities against STEC O103.

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