Phage Therapy as a Tool for Control of Foodborne Diseases: Advantages and Limitations

Pacios‐Michelena, Sandra; Rodríguez‐Herrera, Raúl; Flores‐Gallegos, Adriana C.; González, M.L Chávez; Segura-Ceniceros, Elda Patricia; Ramos‐González, Rodolfo; Ilyiná, Anna

doi:10.2174/9789811412387120080006

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, the scientific community has paid more attention to applying microorganisms as biological control agents for crop protection, which can inhibit or destroy pathogens without harming the environment or compromising consumers' health (Gorai et al., 2021; Pacios‐Michelena et al., 2020; Pacios‐Michelena et al, 2023) . Trichoderma spp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the biocontrol effect of Trichoderma sp. Trs GT2 extracts produced by fermentation processes against Botrytis cinerea Btcr GM3

Saldaña-Mendoza

Chávez‐González

Ramírez-Guzman

et al. 2023

Environmental Quality Mgmt

View full text Add to dashboard Cite

In the present work, the evaluation of the use of Trichoderma spp. for controlling different phytopathogens of agricultural importance (Fusarium oxysporum, Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Colletotrichum gloeosporioides, and Phytophthora sp.) was carried out. Confrontation assays by dual culture, volatile metabolites (overlapping plate assay), and diffusible metabolites (poison medium assay) were performed to identify and select the Trichoderma strain with a greater spectrum of inhibition and the most sensitive phytopathogen. The production of hydrolytic enzymes associated with biological control by the Trichoderma sp. Trs GT2 strain (chitinase, cellulase, amylase, and lipase) was evaluated. Subsequently, the use of solid‐state and submerged fermentation to produce extracts with antagonistic activity against B. cinerea was evaluated, and the production of specific activity β 1–3 glucanase was compared. It was found that Trichoderma sp. Trs GT2 presented the greatest spectrum of inhibition against the phytopathogens evaluated, resulting in the strain of B. cinerea being the most sensitive microorganism to its effects (percentages of inhibition: dual culture 100%, volatile metabolites 100% and diffusible metabolites 28.24%). The Trichoderma sp. Trs GT2 strain showed amylase, cellulase, and chitinase activity. It also produced activity titers β 1–3 glucanase of 13.22 U/g by solid‐state fermentation (SSF) and 8.19 U/mL by submerged fermentation, as well as specific activities of (1.38 U/mg) and (14.23 U/mg), respectively. The extracts produced by both fermentation systems did not present an antagonistic effect against B. cinerea; this can be attributed to the need for interaction with the phytopathogen in question for the induction of metabolites with antagonistic activity, as well as to the confusion that the evaluation of the antagonistic effect of diffusible metabolites, through the cellophane paper technique, can generate in which the mechanism of nutrient competition can cause the inhibitory effect. This work shows that the ability of one microorganism to exert biocontrol against another can vary depending on the mechanism through which it performs and demonstrates that the antagonist‐phytopathogenic interaction can promote the production of metabolites with antagonistic activity.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of the biocontrol effect of Trichoderma sp. Trs GT2 extracts produced by fermentation processes against Botrytis cinerea Btcr GM3

Saldaña-Mendoza

Chávez‐González

Ramírez-Guzman

et al. 2023

Environmental Quality Mgmt

View full text Add to dashboard Cite

show abstract

Encapsulation and application of cell‐free extracts produced by Penicillium chrysogenum R1 in tomato plants for the control of Fusarium oxysporum

Pacios‐Michelena,

Valdés,

Saldaña‐Mendoza

et al. 2024

Environmental Quality Mgmt

View full text Add to dashboard Cite

The application of biopesticides in many industries can be hampered due to their high volatility, susceptibility to degradation when exposed to light or microbial activity, and reduced activity when exposed to stressful environmental conditions. Microencapsulation of biopesticides is a promising technique to solve and address such problems. This study aimed to encapsulate and characterize the extracts produced by Penicillium chrysogenum R1 and evaluate their antifungal activity in the plant assay. The capsules were prepared with a chitosan (0.5 mg/mL) and sodium alginate solution (2% w/v), and 20% (v/v) cell‐free extract recovered from the P. chrysogenum R1 fermentation. The capsules were synthesized in a 2.5% CaCl2 solution at room temperature. The capsules were characterized by DLS and SEM. To determine the efficiency of the free and encapsulated extract, they were applied to tomato plants infected with Fusarium oxysporum. Once the symptoms were observed, the severity and incidence of the disease, as well as the morphological parameters were evaluated. such as dry weight of aerial and root biomass, plant height, root length, stem diameter, chlorophyll content, and number of leaflets. The extract produced by P. chrysogenum R1 was encapsulated in chitosan/alginate capsules with a size range of around 300 nm. The free and encapsulated extracts allowed to efficiently control the disease caused by F. oxysporum and the development of the plants. Therefore, the extract produced by P. chrysogenum R1 encapsulated in chitosan/alginate can effectively control tomato vascular wilt caused by F. oxysporum under greenhouse conditions, promoting plant growth.

show abstract

Phage Therapy as a Tool for Control of Foodborne Diseases: Advantages and Limitations

Cited by 2 publications

References 0 publications

Evaluation of the biocontrol effect of Trichoderma sp. Trs GT2 extracts produced by fermentation processes against Botrytis cinerea Btcr GM3

Evaluation of the biocontrol effect of Trichoderma sp. Trs GT2 extracts produced by fermentation processes against Botrytis cinerea Btcr GM3

Encapsulation and application of cell‐free extracts produced by Penicillium chrysogenum R1 in tomato plants for the control of Fusarium oxysporum

Contact Info

Product

Resources

About