Phage therapy against Vibrio parahaemolyticus infection in the whiteleg shrimp (Litopenaeus vannamei) larvae

Lomelí-Ortega, Carlos Omar; Martínez-Díaz, Sergio F.

doi:10.1016/j.aquaculture.2014.08.018

Cited by 91 publications

(35 citation statements)

References 14 publications

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“…The administration of PAS‐A and PAS‐1 showed notable protective effects in S. senegalensis juveniles and rainbow trout against infection by A. salmonicidai , with increased survival rates and delayed mean death times (Silva et al., ; Kim et al., ). Moreover, use of bacteriophage VPp1 in depuration techniques successfully eliminated V. parahaemolyticus in oysters and shrimp (Lomeli‐Ortega & Martinez‐Diaz, ; Rong, Lin, Wang, Kan, & Li, ). However, only one company (Phage Biotech Ltd., Israel) currently produces phages for use in aquatic culture, and their only product is designed for treatment of V. harveyi in shrimp (Hodgson, ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of bacteriophage HN48 and its protective effects in Nile tilapia Oreochromis niloticus against Streptococcus agalactiae infections

Luo

Guo-li

Liu

et al. 2018

Journal of Fish Diseases

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Streptococcus agalactiae is a causative agent responsible for massive mortalities of tilapia that has led to catastrophic losses to tilapia culture globally. Bacteriophages represent a new class of antimicrobials against bacteria. In this study, we characterized the bacteriophage HN48, which formed small and round-transparent plaques on a double-layer plate. With a hexagonal head and a long tail, this phage may belong to the Caudovirales according to the International Committee on Taxonomy of Viruses. HN48 was found to have a relatively wide and highly specific host range, to be sensitive to high temperature (60-80°C) and low pH (3-5), and to be relatively stable at alkaline pH (8-10). Intraperitoneal injection with HN48 had no adverse effects on tilapia and effectively inactivated the bacteria in the kidney. Fish that received phage therapy had 60% ± 3.3% survival rates and a delayed mean death time of about 3 days when compared to the control group. To the best of knowledge, this is the first study of tilapia streptococcal phage. Overall, the results indicated that phage HN48 could prevent tilapia from experimental S. agalactiae infection, suggesting it has the potential to control this disease.

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Section: Discussionmentioning

confidence: 99%

Characterization of bacteriophage HN48 and its protective effects in Nile tilapia Oreochromis niloticus against Streptococcus agalactiae infections

Luo

Guo-li

Liu

et al. 2018

Journal of Fish Diseases

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“…Vibrio parahaemolyticus is a Gram‐negative bacterium that infects a wide range of aquatic organisms including shrimp (Lomelí‐Ortega & Martínez‐Díaz, ), fish (Khouadja, Lamari, & Bakhrouf, ) and abalone (Liu, Chen, Huang, & Lee, ). In 2013, a unique strain of V. parahaemolyticus harbouring a 69–70 kb plasmid which encodes PirAB‐like toxin (Tran et al, ) was identified as the causative agent of AHPND infecting Penaeus monodon and L. vannamei .…”

Section: Discussionmentioning

confidence: 99%

Anti‐PirA‐like toxin immunoglobulin (IgY) in feeds passively immunizes shrimp against acute hepatopancreatic necrosis disease

Nakamura

Pedrosa-Gerasmio

Alenton

et al. 2019

Journal of Fish Diseases

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Acute hepatopancreatic necrosis disease (AHPND), caused by a toxin‐producing Vibrio parahaemolyticus strain, has become a serious threat to shrimp aquaculture. The need to regulate antibiotic use prompted the development of alternative ways to treat infections in aquaculture including the use of chicken egg yolk immunoglobulin (IgY) for passive immunization. This study evaluated the protective effect of IgY against AHPND infection in Litopenaeus vannamei (Boone). IgY was isolated from eggs laid by hens immunized with recombinant PirA‐like (rPirA) and PirB‐like (rPirB) toxins. Whole‐egg powders having IgY specific to rPirA (anti‐PirA‐IgY) and rPirB (anti‐PirB‐IgY) and IgY from non‐immunized hen (control‐IgY) were mixed with basal diets at 20% concentrations and used to prefeed shrimp 3 days before the bacterial challenge test. Survival rates of the challenged shrimp fed the anti‐PirA‐IgY, anti‐PirB‐IgY and control‐IgY diets were 86%, 14% and 0%, respectively. Only the feed containing anti‐PirA‐IgY protected shrimp against AHPND. Increasing the concentration of rPirA antigen to immunize hens and lowering the amount of egg powder in feeds to 10% consistently showed higher survival rates in shrimp fed with anti‐PirA‐IgY (87%) compared with the control (12%). These results confirm that addition of anti‐PirA‐IgY in feeds could be an effective prophylactic method against AHPND infection in shrimp.

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“…The VPMS1 phage has been used as a study model for the evaluation of the potential of phage therapy for shellfish culture using VP ATCC‐17802 reference strain, which is able to induce mortality in brine shrimp and whiteleg shrimp larvae. Phage treatment was able to prevent vibriosis in Artemia franciscana nauplii and reduce mortality in L. vannamei larvae (Lomelí‐Ortega & Martínez‐Díaz, ; Martínez‐Díaz & Hipólito‐Morales, ).…”

Section: Lytic Effect Of Vpms1 Phage and Muralytic Activity Of Endolymentioning

confidence: 99%

Recombinant LysVPMS1 as an endolysin with broad lytic activity against Vibrio parahaemolyticus strains associated to acute hepatopancreatic necrosis disease

et al. 2018

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Phage therapy against Vibrio parahaemolyticus infection in the whiteleg shrimp (Litopenaeus vannamei) larvae

Cited by 91 publications

References 14 publications

Characterization of bacteriophage HN48 and its protective effects in Nile tilapia Oreochromis niloticus against Streptococcus agalactiae infections

Characterization of bacteriophage HN48 and its protective effects in Nile tilapia Oreochromis niloticus against Streptococcus agalactiae infections

Anti‐PirA‐like toxin immunoglobulin (IgY) in feeds passively immunizes shrimp against acute hepatopancreatic necrosis disease

Recombinant LysVPMS1 as an endolysin with broad lytic activity against Vibrio parahaemolyticus strains associated to acute hepatopancreatic necrosis disease

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