2009
DOI: 10.1007/978-1-60327-164-6_19
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Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes

Abstract: In microbiology, preservation of an archival stock or a "master stock" of a given microorganism is essential for many reasons including scientific research, conservation of the genetic resources and providing the foundation for several biotechnological processes. The objective is to preserve the initial characteristics of the microorganism and to avoid the genetic drift that occurs when the organism is maintained indefinitely in an actively growing state. The same holds true in phage biology and it is of parti… Show more

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Cited by 87 publications
(58 citation statements)
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“…For long‐term storage, bacterial cultures were stored in a 20% glycerol solution at −80°C. Phage was stored at 4°C but for long‐term storage, phage was stored in a 50% glycerol solution at −80°C (Fortier & Moineau, ). S. aureus was grown in brain heart infusion (BHI) media and infected with phage K. For all experiments, E. coli was grown using LB media or LB agar.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…For long‐term storage, bacterial cultures were stored in a 20% glycerol solution at −80°C. Phage was stored at 4°C but for long‐term storage, phage was stored in a 50% glycerol solution at −80°C (Fortier & Moineau, ). S. aureus was grown in brain heart infusion (BHI) media and infected with phage K. For all experiments, E. coli was grown using LB media or LB agar.…”
Section: Methodsmentioning
confidence: 99%
“…Phage was stored at 4°C but for long-term storage, phage was stored in a 50% glycerol solution at −80°C(Fortier & Moineau, 2009). …”
mentioning
confidence: 99%
“…Prophages were induced from C. difficile isolates by UV irradiation (302 nm) (28). Briefly, aliquots of overnight cultures were diluted 10 Ϫ1 , 10 Ϫ2 , and 10 Ϫ3 and further spotted onto the surface of a BHI agar plate and bacteria were allowed to grow in an anaerobic atmosphere for 4 h. Bacteria were then exposed to UV light (302 nm) for 10 s with a transilluminator and overlaid with molten top agar containing a log-phase culture of a C. difficile test strain (optical density at 600 nm of ϳ0.3 to 0.5) (27,34). After overnight incubation under anaerobic conditions, a clearing zone in the bacterial lawn above a given spot was indicative of phage induction and further propagation on the test strain.…”
Section: Methodsmentioning
confidence: 99%
“…When resistance against a given phage occurs, a new phage can be created to target and destroy the new strain. Some protocols on isolation of phage have been mentioned in literature (Garcia et al, 2009; Moineau and Fortier, 2009). The selection and screening a new phage is faster than the development of novel antibiotics which can take up to several years (Sulakvelidze et al, 2001).…”
Section: Introductionmentioning
confidence: 99%